Phospho-IKK beta (Tyr199) Antibody - #AF3010

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产品: 磷酸化 IKK beta (Tyr199) 抗体
货号: AF3010
描述: Rabbit polyclonal antibody to Phospho-IKK beta (Tyr199)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
蛋白号: O14920
RRID: AB_2834449

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Phospho-IKK beta (Tyr199) Antibody detects endogenous levels of IKK beta only when phosphorylated at Tyrosine 199.
RRID:
AB_2834449
引用格式: Affinity Biosciences Cat# AF3010, RRID:AB_2834449.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; IMD15; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta;

抗原和靶标

免疫原:

A synthesized peptide derived from human IKK- beta around the phosphorylation site of Tyr199.

基因/基因ID:
IKBKB(3551)
描述:
IKK-beta is a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Cerebral endothelial cell-derived extracellular vesicles regulate microglial polarization and promote autophagy via delivery of miR-672-5p. Cell death & disease, 2023 (PubMed: 37773169) [IF=8.1]
2). Potential “Therapeutic” Effects of Tocotrienol-Rich Fraction (TRF) and Carotene “Against” Bleomycin-Induced Pulmonary Fibrosis in Rats via TGF-β/Smad, PI3K/Akt/mTOR and NF-κB Signaling Pathways. Nutrients, 2022 (PubMed: 35268069) [IF=5.9]

Application: WB    Species: Rat    Sample:

Figure 8 TRF and carotene protected against pulmonary fibrosis via the inhibition of the NF-κB signaling pathway: (a–d) TRF and carotene decreased the expression of fibrosis proteins induced by BLM in SD rats. The protein expressions of (b) p-p65, (c) p-IkBα, and (d) Ikkβ in lung samples were examined by Western blotting analysis, n = 3; (e–j) representative statistical analyses of (e) TNF-α, (f) IFN-γ, (g) IL-13, (h) NF-κB, (i) IkBα, and (j) Ikkβ mRNA by RT-qPCR, n = 3. Note: compared with CON group, # represents p < 0.05; compared with MOD group, * represents p < 0.05.
3). Anti-Inflammatory Effects of Huangqin Decoction on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice Through Regulation of the Gut Microbiota and Suppression of the Ras-PI3K-Akt-HIF-1α and NF-κB Pathways. Frontiers in Pharmacology, 2020 (PubMed: 32038240) [IF=5.6]

Application: WB    Species: mouse    Sample: colorectal

FIGURE 7 | Effects of Huangqin decoction (HQD) on colonic expression levels of nuclear factor-kappa B (NF-kB) pathway proteins assessed by Western blot in dextran sulfate sodium (DSS)-induced colitis mice (A).
4). Tocotrienol-Rich Fractions Offer Potential to Suppress Pulmonary Fibrosis Progression. International Journal of Molecular Sciences, 2022 (PubMed: 36430808) [IF=5.6]

Application: WB    Species: Rat    Sample: lung

Figure 11 PF was protected by TRF via the inhibition of the NF-κB signaling pathway: (a–d) The expression of fibrosis proteins induced by BLM in SD rats was decreased; protein expressions of p-IkBα (b), Ikkβ (c) and p-p65 (d) in lung samples were examined by WB analysis; n = 3. (e–j) Representative statistical analyses of TNF-α (e), IFN-γ (f), IL-13 (g), IkBα (h), NF-κB (i) and Ikkβ (j) in mRNA by RT-qPCR; n = 3. Note: * represents p < 0.05 in comparison to the control group; # represents p < 0.05 in comparison to the model group.
5). Nr2e1 deficiency aggravates insulin resistance and chronic inflammation of visceral adipose tissues in a diet-induced obese mice model. LIFE SCIENCES, 2021 (PubMed: 33915130) [IF=5.2]

Application: WB    Species: mice    Sample: adipose tissues

Fig. 5. Nr2e1 deficiency exacerbated inflammation in EAT. Comparison of the epididymal fat weight (A). The ratio of epididymal fat weight to the total body weight (B). The mRNA expression of F4/80 (C). The mRNA and protein expressions of IL-6, IL-1β, TNF-α and MCP-1 (E, F). The protein levels of p-IKKβ/IKKβ, p-P65/P65 (G, H). The data are expressed as means ± SEM. *P < 0.05, **P < 0.01. ns: not significant.
6). Mechanism of Astragalus mongholicus Bunge ameliorating cerebral ischemia-reperfusion injury: Based on network pharmacology analysis and experimental verification. Journal of ethnopharmacology, 2024 (PubMed: 38588987) [IF=4.8]
7). Caveolin-1 aggravates neurological deficits by activating neuroinflammation following experimental intracerebral hemorrhage in rats. Experimental Neurology, 2023 (PubMed: 37598879) [IF=4.6]
8). Discovery of a doublecortin-like kinase 1 inhibitor to prevent inflammatory responses in acute lung injury. Bioorganic chemistry, 2024 (PubMed: 38394920) [IF=4.5]
9). Nr2e1 ablation impairs liver glucolipid metabolism and induces inflammation, high-fat diets amplify the damage. Biomedicine & Pharmacotherapy, 2019 (PubMed: 31590127) [IF=4.0]

Application: WB    Species: mouse    Sample: liver

Fig. 5. |Nr2e1 ablation induced hepatic inflammation, the change was aggravated by HFD treatment.The expressions of phosphorylated protein of IKKβ, P65, IκBα in liver samples from the four groups were detected by western blots (n = 3) (5A).
10). Nuclear Localization Leucine-Rich-Repeat Protein 1 Deficiency Protects Against Cardiac Hypertrophy by Pressure Overload. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018 (PubMed: 30001530) [IF=2.5]

Application: WB    Species: mouse    Sample: heart

Fig. 5.| Effect of NLRP1 on NF-kB signaling in vivo and in vitro. (a) The mRNA expressions of IL-1β, IL10, and TNF-α in the myocardium were assessed in the indicated groups using real-time PCR (n=6). (b) Representative western blot analysis of phosphorylated and total inhibitor of κB kinase-β (IKKβ), inhibitor of NF-κBα (IκBα), and NF-κBp65 in the heart tissues of mice from the indicated groups (n=6). Top, representative blots; bottom, quantitative results. Values are the mean ± SEM. *P<0.05 compared with the corresponding sham group. #P<0.05 vs WT+AB group.
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