Phospho-IKK beta (Tyr188) Antibody - #AF3009

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产品: 磷酸化 IKK beta (Tyr188) 抗体
货号: AF3009
描述: Rabbit polyclonal antibody to Phospho-IKK beta (Tyr188)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
蛋白号: O14920
RRID: AB_2834448

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Phospho-IKK beta (Tyr188) Antibody detects endogenous levels of IKK beta only when phosphorylated at Tyrosine 188.
RRID:
AB_2834448
引用格式: Affinity Biosciences Cat# AF3009, RRID:AB_2834448.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; IMD15; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta;

抗原和靶标

免疫原:

A synthesized peptide derived from human IKK- beta around the phosphorylation site of Tyr188.

基因/基因ID:
IKBKB(3551)
描述:
IKK-beta is a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Overproduction of Gastrointestinal 5-HT Promotes Colitis-Associated Colorectal Cancer Progression via Enhancing NLRP3 Inflammasome Activation. Cancer Immunology Research, 2021 (PubMed: 34285037) [IF=8.1]

Application: WB    Species: Human and mouse    Sample: SW620, HT29, and CT26 cells

Figure 6.5-HT biosynthesis in colorectal cancer cells is upregulated by IL1β release in macrophages. A, Experiment design: Macrophages were activated by LPS (100 ng/mL, 4 hours) and ATP (5 mmol/L, 1 hour) with/without the IL1β production inhibitor Dia (10 μmol/L) for 5 hours, and then medium was replaced by fresh medium for another 6-hour incubation. Supernatants were then collected and used to incubate colorectal cancer (CRC) cells for 24 hours. mRNA expression of TPH1/Tph1 was detected by real-time PCR (B), and 5-HT concentration in supernatants was detected by ELISA (C) for SW620, HT29, and CT26 cells after incubation with conditional medium (CM) from activated macrophages (A-THP-1 CM, A-iBMDM CM) with/without Dia treatment. D, Protein expression of phospho-IKKβ (Tyr 188), IKKβ, phospho-NFκB p65 (Ser 536), NFκB p65, and TPH1 were detected by Western blotting in SW620, HT29, and CT26 cells after incubation with CM from activated macrophages (A-Φ CM; THP-1 for SW620/HT29 and iBMDM for CT26) with/without Dia treatment. mRNA expression of TPH1 (E) and 5-HT concentration in supernatants (G) from SW620 cells after incubation with CM from activated THP-1 cells with/without IL1RA (0.1 μg/mL), TLR1 (100 μmol/L), or BMS-345541 (4 μmol/L) for 24 hours. mRNA expression of TPH1 (F) and 5-HT concentration in supernatants (H) from HT29 cells after incubation with CM from activated THP-1 with/without IL1RA (0.1 μg/mL), TLR1 (100 μmol/L), and BMS-345541 (4 μmol/L) treatment for 24 hours. I, mRNA expression of Tph1 (left) and 5-HT concentration in supernatants (right) from CT26 cells after incubation with CM from activated iBMDM cells for 24 hours, and with/without TLR1 (100 μmol/L) or BMS-345541 (4 μmol/L) treatment. B, C, and E–I, Data represent mean ± SD for at least three independent experiments; one-way ANOVA. J, Representative IHC photos for TPH1 in tumor tissues from the CT26 and iBMDM coimplanted subcutaneous allograft colorectal cancer mouse model (see Fig. 7). Scale bar: 50 μm. WT, wild-type. **, P < 0.01. n.s., no significant differences.
2). Antidepressant-like effects of helicid on a chronic unpredictable mild stress-induced depression rat model: Inhibiting the IKK/IκBα/NF-κB pathway through NCALD to reduce inflammation. International Immunopharmacology, 2021 (PubMed: 33578182) [IF=4.8]

Application: WB    Species: rat    Sample: hippocampus

Fig. 10. The effects of the IKK/IκBα/NF-κB signaling pathway in the hippocampus after AAV-siNCALD. (A) Representative NCALD, IKKβ, p-IKKβ, IкBα, p- IкBα, NF-кB, β-actin, and Lamin B western blot bands. (B), (C), (D), (E), (F), (G), (H) The relative ratios of the grayscale values of NCALD, IKKβ, p-IKKβ, IкBα, pIкBα, and NF-кB to the corresponding β-actin or Lamin B, n = 4. ###P < 0.001, CUMS + saline vs. sham; *P < 0.05; **P < 0.01; ***P < 0.001, CUMS + saline vs. CUMS + AAV-siNCALD. All data are shown as the mean ± SEM.
3). Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions. Iranian Journal of Basic Medical Sciences, 2023 [IF=2.1]

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