AKT1 Antibody - #AF0836

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产品: AKT1 抗体
货号: AF0836
描述: Rabbit polyclonal antibody to AKT1
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Dog, Chicken, Xenopus
蛋白号: P31749
RRID: AB_2834120

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
AKT1 Antibody detects endogenous levels of total AKT1.
RRID:
AB_2834120
引用格式: Affinity Biosciences Cat# AF0836, RRID:AB_2834120.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AKT 1; AKT1; AKT1_HUMAN; MGC99656; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha;

抗原和靶标

免疫原:

A synthesized peptide derived from human AKT1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
AKT1(207)
描述:
an AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway. Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. First found in a mouse transforming retrovirus. Tumorigenic in a mouse lymphoma model and activated (by phospho-Akt staining) and/or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Inhibitor: RX-0201. Substrates include tuberin, Bad, Forkhead transcription factors, caspase-9, and glycogen synthase kinase-3.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

文献引用

1). Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis. Cell stem cell, 2024 (PubMed: 38232727) [IF=19.8]
2). NELL2, a novel osteoinductive factor, regulates osteoblast differentiation and bone homeostasis through fibronectin 1/integrin-mediated FAK/AKT signaling. Bone Research, 2025 [IF=15.0]
3). N6-methyladenosine-associated prognostic pseudogenes contribute to predicting immunotherapy benefits and therapeutic agents in head and neck squamous cell carcinoma. Theranostics, 2023 (PubMed: 36438489) [IF=12.4]

Application: WB    Species: Human    Sample: ARO and Tca8113 cell

Figure 4 m6A-associated pseudogene can regulate targeted immune-involved genes via miRNAs. (A) Sankey showing pseudogenes together with binding miRNAs and target genes with | R | ≥ 0.3 and P < 0.05 were used to construct the pseudogene-miRNA-target gene regulatory networks by subtypes of oncogene pseudogene PDIA3P1 and tumor-suppressor pseudogene RRN3P3. The column on the left represented pseudogenes, which are located at the cores of the networks. The column in the middle and the column on the right stand for binding miRNAs and target genes, respectively. (B-G) Experimental validation of PDIA3P1 affects the expression of AKT1 via miR-34a-5p in ARO and Tca8113 cell lines. (B) Relative gene expression of PDIA3P1 after PDIA3P1 knockdown using siRNA. (C) Relative gene expression of AKT1 after PDIA3P1 knockdown using siRNA. (D) Western blot comparing the protein levels of AKT1 in control and PDIA3P1 knockdown cells. (E) The relative expression of AKT1 at different time points after transcription inhibition in control and PDIA3P1 knockdown cells respectively. Error bars represent standard errors. (F) The relative expression of PDIA3P1 and AKT1 after adding control inhibitor versus miR-34a-5p inhibitor. (G) Western blot comparing the protein levels of AKT1 after adding control inhibitor versus miR-34a-5p inhibitor. (H-M) Experimental validation of RRN3P3 affects the expression of EZH2 via miR-26b-5p in ARO and Tca8113 cell lines. (H) Relative gene expression of RRN3P3 after RRN3P3 knockdown using siRNA. (I) Relative gene expression of EZH2 after RRN3P3 knockdown using siRNA. (J) The relative expression of EZH2 at different time points after transcription inhibition in control and RRN3P3 knockdown cells respectively. Error bars represent standard errors. (K) Western blot comparing the protein levels of EZH2 in control and RRN3P3 knockdown cells. (L) The relative expression of RRN3P3 and EZH2 after adding control inhibitor versus miR-26b-5p inhibitor. (M) Western blot comparing the protein levels of EZH2 after adding control inhibitor versus miR-26b-5p inhibitor. (N) UCSC genome browser tracks m6A-seq and m6A-LAIC-seq data indicating m6A peaks and m6A levels of oncogene pseudogene PDIA3P1 and tumor-suppressor pseudogene RRN3P3. Read-coverage tracks of input, m6A-negative, and m6A-positive fractions of m6A-LAIC-seq shown along with overlay tracks of m6A-seq (cyan for input and red for RIP; predicted m6A sites in m6A peaks are indicated by arrows). Read coverage (y-axis) of m6A negative and m6A positive are normalized as previously described 17 to reflect the calculated m6A levels (i.e., equal signals in m6A positive (eluate) versus m6A negative (supernatant) suggest m6A levels of 50%), while input and IP tracks of m6A-seq are shown for optimal viewing at the top panel. * P< 0.05; ** P< 0.01; *** P< 0.001 (two-tailed t-test). (O) The m6A methylation level of the pseudogenes at specific modification sites (Chr1:146650342, GG(m6A)CA on PDIA3P1; Chr16:22431201, GG(m6A)CG on RRN3P3) using SELECT in control and METTL3 knockdown ARO and Tca8113 cell.
4). MiR-146b-5p enriched bioinspired exosomes derived from fucoidan-directed induction mesenchymal stem cells protect chondrocytes in osteoarthritis by targeting TRAF6. Journal of nanobiotechnology, 2023 (PubMed: 38105181) [IF=10.2]

Application: WB    Species: Rat    Sample: chondrocytes

Fig. 7 Enriched miR-146b-5p in F-MSCs-Exo inhibits PI3K/AKT/mTOR pathway by targeting TRAF6. (A, B) Western blot analysis was performed to detect the impact of F-MSCs-Exo on TRAF6 and the PI3K/AKT/mTOR pathway in rat chondrocytes. (C, D) The expression of TRAF6 was quantitatively analyzed using immunofluorescence staining and ImageJ software (scale bar = 10 μm). (E, F) Direct visualization of chondrocytes treated with nc-inhibitor and miR-146b-5p-inhibitor was performed using Alcian blue staining and safranin staining. (G, H) Western blot analysis was conducted to examine the expressions of TRAF6 and the PI3K/AKT/mTOR pathway in chondrocytes after treatment with nc-inhibitor and miR-146b-5p-inhibitor. (ns, no significant difference; *p 
5). Di-2-ethylhexyl phthalate (DEHP) exposure induces sperm quality and functional defects in mice. Chemosphere, 2023 (PubMed: 36372335) [IF=8.1]
6). The role of miR-222-2p in exosomes secreted by hexavalent chromium-induced premature senescent hepatocytes as a SASP component. Environmental pollution (Barking, Essex : 1987), 2024 (PubMed: 38365080) [IF=7.6]
7). XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36791564) [IF=6.9]
8). Mechanistic insights into the potentiation and toxicity mitigation of myocardial infarction treatment with salvianolate and ticagrelor. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 40209643) [IF=6.7]
9). Effect of resveratrol on herpesvirus encephalitis: Evidences for its mechanisms of action. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38430586) [IF=6.7]
10). Fraxetin pretreatment alleviates cisplatin-induced kidney injury by antagonizing autophagy and apoptosis via mTORC1 activation. Phytotherapy research : PTR, 2024 (PubMed: 38558449) [IF=6.1]
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