Phospho-AKT1 (Thr308) Antibody - #AF0832

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产品: 磷酸化 AKT1 (Thr308) 抗体
货号: AF0832
描述: Rabbit polyclonal antibody to Phospho-AKT1 (Thr308)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
蛋白号: P31749
RRID: AB_2834106

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-AKT1 (Thr308) Antibody detects endogenous levels of AKT1 only when phosphorylated at Threonine 308.
RRID:
AB_2834106
引用格式: Affinity Biosciences Cat# AF0832, RRID:AB_2834106.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AKT 1; AKT; AKT1; AKT1_HUMAN; MGC99656; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha;

抗原和靶标

免疫原:

A synthesized peptide derived from human AKT1 around the phosphorylation site of Thr308.

基因/基因ID:
AKT1(207)
描述:
an AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway. Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. First found in a mouse transforming retrovirus. Tumorigenic in a mouse lymphoma model and activated (by phospho-Akt staining) and/or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Inhibitor: RX-0201. Substrates include tuberin, Bad, Forkhead transcription factors, caspase-9, and glycogen synthase kinase-3.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

文献引用

1). The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML. Blood Cancer Journal, 2021 (PubMed: 34099621) [IF=12.9]

Application: WB    Species: Human    Sample: MV4-11 cells

Fig. 5 CUDC-907 and gilteritinib induce complimentary and cooperatively altered activity within the MAPK/ERK and JAK2/STAT5 pathways. A MV4-11 cells were treated with gilteritinib, CUDC-907, both, or neither for 4, 8, 12, or 24 h. Western blots were generated utilizing whole-cell lysates, with representative blots shown, and densitometry displayed below each blot. Densitometry was assessed via comparison to vehicle control and normalized to β-actin. B A primary FLT3-ITD positive AML patient sample was treated with gilteritinib and/or CUDC-907 for 24 h. Densitometry was assessed via comparison to vehicle control and normalized to β-actin. C, D MOLM-13 and MV4-11 cells were treated using CUDC-907 either with or without AZD1480, a selective JAK2 inhibitor, for 24 h. Flow cytometry analysis of annexin V/PI stained cells is shown in the upper panels and western blot analyses of phosphorylated STAT5 are shown in the lower panels. ***p < 0.001 compared to single-drug treatments. E MOLM-13, MV4-11, and primary patient sample AML#213 were treated with gilteritinib, CUDC-907, both, or vehicle control for 24 h. Western blotting was performed to analyze expression of members of the Bcl-2 family. Densitometry was measured via comparison to vehicle control and normalized to β-actin. F Mcl-1 overexpression and red fluorescent protein (RFP) control MV4-11 cells were generated using lentivirus particles as described in the “Methods” section. Whole-cell lysates were subjected to western blotting to confirm overexpression (upper panel). The cells were then treated with vehicle control, gilteritinib, CUDC-907, or in combination for 24 h, and then annexin V/PI staining and flow cytometry analysis were performed (lower panel). ***p < 0.001 compared to RFP under the same drug treatment. G, H shRNA knockdown of Bim, Bak, and Bax, or non-template control (NTC) was performed in MV4-11 cells. Whole-cell lysates were subjected to western blotting (upper panels). Cells were treated with vehicle control, gilteritinib, CUDC-907, or in combination for 24 h. Annexin V/PI staining and flow cytometry analysis results are shown (lower panels). ***p < 0.001 compared to NTC for the same drug treatment.
2). Di-2-ethylhexyl phthalate (DEHP) exposure induces sperm quality and functional defects in mice. Chemosphere, 2023 (PubMed: 36372335) [IF=8.1]
3). Buqi Huoxue Tongnao prescription protects against chronic cerebral hypoperfusion via regulating PI3K/AKT and LXRα/CYP7A1 signaling pathways. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38959552) [IF=6.7]
4). Protective effect of remdesivir against pulmonary fibrosis in mice. Frontiers in Pharmacology, 2021 (PubMed: 34512328) [IF=5.6]

Application: WB    Species: Mice    Sample: CAGA-NIH3T3 cells

FIGURE 6 Remdesivir inhibits TGF-β1-induced activation of Smad and non-Smad signaling pathway in lung fibroblasts (A) Luciferase assays of CAGA-NIH3T3 cells. Cells were pretreated with Remdesivir (0–50 μM) for 30 min and then incubated with TGF-β1 (5 ng ml−1) for 24 h, then analyzed with luciferase assay. SB431542 is a TGF-β1/Smad pathway inhibitor and serves as a positive control (B) NIH-3T3 cells were co-treated with TGF-β1 (5 ng ml−1) and Remdesivir (12.5, 25, 50 μM) for 1 h. P-Smad3 and Smad3 were assessed using western blot. GAPDH was used as the internal control (C) PPF cells were co-treated with TGF-β1 (5 ng ml−1) and Remdesivir (12.5, 25, 50 μM) for 1 h. P-Smad3 and Smad3 were assessed using western blot. GAPDH was used as the internal control (D) NIH-3T3 cells were co-treated with TGF-β1 (5 ng ml−1) and Remdesivir (12.5, 25, 50 μM) for 1 h and the phosphorylation levels of P-38, JNK, ERK and Akt were analyzed by Western blot. β-tubulin was used as a loading control in grayscale analysis. Scale bar = 60 μm. Data was presented as the means ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
5). Hua-Tan-Sheng-Jing Decoction Treats Obesity With Oligoasthenozoospermia by Up-Regulating the PI3K-AKT and Down-Regulating the JNK MAPK Signaling Pathways: At the Crossroad of Obesity and Oligoasthenozoospermia. Frontiers in Pharmacology, 2022 (PubMed: 35559247) [IF=5.6]
6). 8-Gingerol Ameliorates Myocardial Fibrosis by Attenuating Reactive Oxygen Species, Apoptosis, and Autophagy via the PI3K/Akt/mTOR Signaling Pathway. Frontiers in Pharmacology, 2021 (PubMed: 34393792) [IF=5.6]

Application: WB    Species: Mouse    Sample: cardiac tissue

FIGURE 8 Effects of 8-Gin on the expression of the PI3K/AKT/mTOR signaling pathway relevant proteins and MMP-9 protein. (A) Representative images of PI3K/AKT/mTOR signaling pathway protein and MMP-9 protein expression were exhibited from cardiac tissue in the CON, ISO, 8-GinL + ISO, 8-GinH + ISO, and CAP + ISO groups. (B–D) Pooled analysis of PI3K, p-PI3K, AKT, p-AKT, mTOR, and p-mTOR protein expression by western blot analysis on which intensity was normalized to GAPDH. (E) Pooled analysis of MMP-9 protein expression by western blot analysis on which intensity was normalized to GAPDH. Data are shown as the mean ± SEM, n = 3. ** p < 0.01 vs. the CON group; # p < 0.05, ## p < 0.01 vs. the ISO group.
7). KIRREL promotes the proliferation of gastric cancer cells and angiogenesis through the PI3K/AKT/mTOR pathway. Journal of cellular and molecular medicine, 2024 (PubMed: 37909722) [IF=5.3]
8). Regulation of optimized new Shengmai powder on cardiomyocyte apoptosis and ferroptosis in ischemic heart failure rats: The mediating role of phosphatidylinositol-3-kinase/protein kinase B/tumor protein 53 signaling pathway. Journal of ethnopharmacology, 2024 (PubMed: 38692417) [IF=4.8]
9). Ginsenoside Rd Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Inflammation and Apoptosis through PI3K/Akt Signaling Pathway. The American journal of Chinese medicine, 2024 (PubMed: 38577825) [IF=4.8]
10). Molybdenum interferes with MMPs/TIMPs expression to reduce the receptivity of porcine endometrial epithelial cells. Chemico-biological interactions, 2025 (PubMed: 39486568) [IF=4.7]
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