WB, IHC, IF/ICC, ELISA

Hm, Ms, Rt, Pg, Zf, Bv, Rb, Dg, Mk, Fs

货号	产品	规格	来源	应用	反应	文献引用
AF5402	LC3A/B Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	84
AF5128	Beclin 1 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	60
AF7587	ATG3 Ab	20ul	Rabbit	WB	Hm,Ms,Rt	0
DF6010	APG5L/ATG5 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	13
DF6130	ATG7 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	13
DF7937	ATG12 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	2
DF3825	ATG16L1 Ab	20ul	Rabbit	WB,IF/ICC	Hm,Ms	1
AF7018	beta Actin Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Pg,Zf,Bv,Rb,Dg,Mk,Fs	611
S0001	Goat Anti-Rabbit IgG HRP	100ul	Goat	WB,IHC,ELISA	Rb	454

Autophagy is a highly conserved cellular process responsible for the degradation and recycling of cellular components, including damaged organelles, protein aggregates, and intracellular pathogens. It plays a critical role in maintaining cellular homeostasis, adaptation to stress conditions, and cellular survival.

Store at -20 °C. Stable for 12 months from date of receipt.

The autophagy pathway involves several steps:

1. **Initiation:** Autophagy is initiated by the formation of a specialized double-membrane structure called the phagophore or isolation membrane. This process is regulated by various signaling pathways, including the mTOR (mechanistic target of rapamycin) pathway, which inhibits autophagy under nutrient-rich conditions.

2. **Elaboration:** The phagophore expands and engulfs cellular cargo, forming a double-membrane vesicle called the autophagosome. This process involves the coordinated action of autophagy-related (ATG) proteins, including the ATG12-ATG5-ATG16L1 complex and the microtubule-associated protein 1A/1B-light chain 3 (LC3) protein.

3. **Maturation:** The autophagosome fuses with lysosomes to form an autolysosome, where the sequestered cargo is degraded by lysosomal hydrolases. This step is essential for the breakdown of cargo and recycling of macromolecules.

4. **Termination:** Once degradation is complete, the autolysosome disintegrates, and the resulting breakdown products are released back into the cytoplasm for reuse.

Autophagy is regulated by a complex interplay of signaling pathways and cellular stress responses, including nutrient availability, energy status, oxidative stress, and growth factor signaling. Dysregulation of autophagy has been implicated in various diseases, including cancer, neurodegenerative disorders, metabolic disorders, and infectious diseases. Therefore, understanding the autophagy pathway and its regulation is crucial for the development of therapeutic strategies targeting autophagy-related diseases.