Beclin 1 Antibody - #AF5128

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产品: Beclin 1 抗体
货号: AF5128
描述: Rabbit polyclonal antibody to Beclin 1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q14457
RRID: AB_2837614

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Beclin 1 Antibody detects endogenous levels of total Beclin 1.
RRID:
AB_2837614
引用格式: Affinity Biosciences Cat# AF5128, RRID:AB_2837614.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APG6; ATG 6; ATG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; Beclin 1 (coiled coil moesin like BCL2 interacting protein); Beclin 1 autophagy related; Beclin-1; Beclin1; BECN 1; Becn1; BECN1_HUMAN; Coiled coil myosin like BCL2 interacting protein; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS 30; VPS30;

抗原和靶标

免疫原:

A synthesized peptide derived from human Beclin 1, corresponding to a region within the internal amino acids.

基因/基因ID:
BECN1(8678)
描述:
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

文献引用

1). Gold nanoparticles targeting the autophagy–lysosome system to combat the inflammation-compromised osteogenic potential of periodontal ligament stem cells: From mechanism to therapy. Biomaterials, 2022 (PubMed: 36030103) [IF=12.8]
2). Chondrocyte-targeted bilirubin/rapamycin carrier-free nanoparticles alleviate oxidative stress and modulate autophagy for osteoarthritis therapy. Journal of controlled release : official journal of the Controlled Release Society, 2024 (PubMed: 39701459) [IF=10.8]
3). NCAPD2 inhibits autophagy by regulating Ca2+/CAMKK2/AMPK/mTORC1 pathway and PARP-1/SIRT1 axis to promote colorectal cancer. CANCER LETTERS, 2021 (PubMed: 34229059) [IF=9.1]

Application: WB    Species: Human    Sample: CRC cells

Fig. 2. NCAPD2 inhibited cell autophagy and disrupted autophagic flux via Ca2+/CAMKK2/AMPK/mTORC1 pathway. (A) Western blot analyses for phosphorylated mTOR (p-mTOR, S2448), phosphorylated p70S6K (p-p70S6K, T389/412), phosphorylated 4E-BP1 (p-4E-BP1, T70) and phosphorylated AKT (p-AKT, S473) in CRCC cells with different treatments as indicated. (B) Western blot of indicated proteins in cells treated with mTORC1 inhibitor Rapamycin (3 nM, 24h). (C) Immunofluorescence staining of LC3II (red) and P62 (red) in CRC cells with different treatments as indicated. Merged images represented overlays of LC3II or P62 and nuclear staining by DAPI (blue). (D) Intracellular Ca2+ levels were analyzed by flow cytometry after staining with the fluorescent probe Fluo-3, AM in CRC cells. (E) Representative Western blot gel documents of phosphorylated CAMKK2(S511), phosphorylated AMPK(T172), phosphorylated mTOR(S2448), Beclin, ATG5, P62, LC3II in CRC cells with different treatments. (F) Western blots of indicated proteins in cells treated with an inhibitor of microsomal Ca2+-ATPase Thapsigargin (1 μM, 6h) and Ca2+ chelator BAPTA-AM (10 μM, 12h) respectively. Results are shown as mean ± s.d, *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t-test. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
4). Enhancing retention and permeation of rapamycin for osteoarthritis therapy using a two-stage drug delivery system. Materials today. Bio, 2024 (PubMed: 39403313) [IF=8.7]
5). Identification of walnut-derived peptide WNP-8 as a potential therapeutic agent and SIRT2 as a biomarker for cognitive deficiency: A label-free LC-MS/MS proteomics study. International journal of biological macromolecules, 2024 (PubMed: 39647753) [IF=7.7]
6). Polysaccharide from Strongylocentrotus nudus eggs regulates intestinal epithelial autophagy through CD36/PI3K-Akt pathway to ameliorate inflammatory bowel disease. International Journal of Biological Macromolecules, 2023 (PubMed: 37327932) [IF=7.7]
7). Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail. Cell and Bioscience, 2021 (PubMed: 34481526) [IF=7.5]

Application: WB    Species: human    Sample: MDA-MB-231 cells

Fig. 2 |PS-T reverses EMT by inducing autophagy. a MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each autophagy marker in MDA-MB-231 cells are quantifed using the NIH ImageJ software. (mean ± SD, *P < 0.05 and ***P < 0.001).

Application: WB    Species: Human    Sample: MDA-MB-231 cells

Fig. 2 PS-T reverses EMT by inducing autophagy. a MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each autophagy marker in MDA-MB-231 cells are quantified using the NIH ImageJ software. (mean  ±  SD, *P  <  0.05 and ***P  <  0.001). b Confocal microscopy images of cells treated with or without PS-T (5 μg/mL) for 24 h after transfection with mRFP-GFP-LC3 plasmid (scale bars: 10 μm). Quantification of LC3-GFP and LC3-RFP puncta/cells from three independent experiments (***P  <  0.001). c MDA-MB-231 cells are treated with PS-T (5 μg/mL) for 24 h with or without LY294002 (10 µM). The levels of each EMT marker in MDA-MB-231cells were quantified using NIH ImageJ software. (mean  ±  SD, *P  <  0.05 and **P  <  0.01)
8). MiR-34a inhibitor protects mesenchymal stem cells from hyperglycaemic injury through the activation of the SIRT1/FoxO3a autophagy pathway. Stem Cell Research & Therapy, 2020 (PubMed: 33546760) [IF=7.5]

Application: WB    Species: mouse    Sample: MSCs

Fig. 3 |MiR-34a influences the expression of autophagy factors under hyperglycaemic conditions. MSCs were cultured with normal or high glucose medium for 28 days with or without transfection with miR-34a mimic, NC or miR-34a inhibitor for 48 h, respectively. a, b The protein level of SIRT1 and FoxO3a, and autophagy-related protein LC3 and Beclin1 were determined by western blot.
9). RETRACTED ARTICLE: MiR-34a inhibitor protects mesenchymal stem cells from hyperglycaemic injury through the activation of the SIRT1/FoxO3a autophagy pathway. Stem Cell Research & Therapy, 2021 (PubMed: 33546760) [IF=7.5]

Application: WB    Species: Rat    Sample:

Fig. 3 MiR-34a influences the expression of autophagy factors under hyperglycaemic conditions. MSCs were cultured with normal or high glucose medium for 28 days with or without transfection with miR-34a mimic, NC or miR-34a inhibitor for 48 h, respectively. a, b The protein level of SIRT1 and FoxO3a, and autophagy-related protein LC3 and Beclin1 were determined by western blot. c Autophagic corpuscle was detected by TEM. Data are presented as mean ± SD of three separate experiments. *P 
10). Geniposide ameliorates glucocorticoid-induced osteoblast apoptosis by activating autophagy. Biomedicine & Pharmacotherapy, 2022 (PubMed: 36271582) [IF=6.9]

Application: WB    Species: Rat    Sample: MC3T3-E1 cells

Fig. 3. The effects of GEN on autophagy in DEX-treated osteoblasts in vivo and in vitro. The immunohistochemical changes (× 40 magnification) of P62 (A, B), LC3-II (A, C), and Beclin-1 (A, D) were analyzed. The proteins expression of P62 (E, F), LC3-II (E, G), and Beclin-1 (E, H) in DEX-treated MC3T3-E1 cells were determined by western blot. * *P˂0.01. NC, negative control group; DEX, model rat group treated with DEX (5 mg/kg).

Application: IHC    Species: Rat    Sample: MC3T3-E1 cells

Fig. 3. The effects of GEN on autophagy in DEX-treated osteoblasts in vivo and in vitro. The immunohistochemical changes (× 40 magnification) of P62 (A, B), LC3-II (A, C), and Beclin-1 (A, D) were analyzed. The proteins expression of P62 (E, F), LC3-II (E, G), and Beclin-1 (E, H) in DEX-treated MC3T3-E1 cells were determined by western blot. * *P˂0.01. NC, negative control group; DEX, model rat group treated with DEX (5 mg/kg).
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