ATG7 Antibody - #DF6130

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(17)   (10)  
产品: ATG7 抗体
货号: DF6130
描述: Rabbit polyclonal antibody to ATG7
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: O95352
RRID: AB_2838097

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ATG7 Antibody detects endogenous levels of total ATG7.
RRID:
AB_2838097
引用格式: Affinity Biosciences Cat# DF6130, RRID:AB_2838097.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1810013K23Rik; Apg 7; APG7 autophagy 7 like; APG7 autophagy 7-like (S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7-like; APG7L; ATG 7; ATG12-activating enzyme E1 ATG7; ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7 autophagy related 7 homolog; ATG7_HUMAN; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7 (yeast); Autophagy-related protein 7; DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7;

抗原和靶标

免疫原:

A synthesized peptide derived from human ATG7, corresponding to a region within N-terminal amino acids.

基因/基因ID:
ATG7(10533)
描述:
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

文献引用

1). Calycosin inhibited autophagy and oxidative stress in chronic kidney disease skeletal muscle atrophy by regulating AMPK/SKP2/CARM1 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 32910538) [IF=5.3]

Application: IHC    Species: rat    Sample: muscles

FIGURE 4|Effect of calycosin on autophagy in muscle of 5/6 Nx rats. A, Representative images of autophagosomes observed in muscle of rat using TEM. B-E, Representative images and scores of LC3A/B and ATG7 expression in muscle of rats using IHC staining (×200). Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group

Application: WB    Species: rat    Sample: muscles

FIGURE 5|Effect of calycosin on the expression of CARM1 and H3R17me2a,and related proteins in rat muscles. A-B,Representative images and scores of the expression of CARM1 and H3R17me2a in muscle sections using IHC staining (×200).Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group. C-E, Representative immune blots of key proteins involved in muscle atrophy and proteins associated with the AMPK/SKP2/CARM1 signalling pathway
2). Integrin 51, as a Receptor of Fibronectin, Binds the FbaA Protein of Group A Streptococcus To Initiate Autophagy during Infection. mBio, 2020 (PubMed: 32518187) [IF=5.1]
3). Lupeol inhibits the proliferation and migration of MDA-MB-231 breast cancer cells via a novel crosstalk mechanism between autophagy and the EMT. Food & Function, 2022 (PubMed: 35448900) [IF=5.1]
4). Qingyihuaji Formula promotes apoptosis and autophagy through inhibition of MAPK/ERK and PI3K/Akt/mTOR signaling pathway on pancreatic cancer in vivo and in vitro. Journal of Ethnopharmacology, 2023 (PubMed: 36690307) [IF=4.8]
5). Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. Journal of Virology, 2018 (PubMed: 29386287) [IF=4.0]

Application: WB    Species: human    Sample: Hep2 cells

Figure 5. |Inhibition of autophagy with specific shRNA targeting ATG5, ATG7 or BECN1 reduces RSV replication. (A-C) Hep2 cells stably expressing specific shRNA against ATG5 (Hep2-sh-ATG5), ATG7 (Hep2-sh-ATG7), BECN1 (Hep2-sh-BECN1) or luciferase (Hep2-sh-luciferase) were established as described in Materials and Methods. The silencing efficiency of ATG5 shRNA (A), ATG7 shRNA (B) and BECN1 shRNA (C) were validated by immunoblotting with anti-ATG5, anti-ATG7, anti-BECN1 and anti-GAPDH antibodies respectively.
6). Pelargonic acid vanillylamide alleviates hepatic autophagy and ER stress in hepatic steatosis model. Food and Chemical Toxicology, 2023 (PubMed: 37611858) [IF=3.9]
7). Alginate oligosaccharides improve high-fat induced hepatic steatosis via PGC-1α-mediated lipophagy and fatty acid β-oxidation pathway. Journal of Functional Foods, 2023 [IF=3.8]

Application: WB    Species: Mouse    Sample: livers

Fig. 4. Effects of AOS on enhancing the lipophagy and free fatty acid (FFA) β-oxidation level in the NAFLD mouse livers. (A) the mRNA expression in the lipophagy and FFA β-oxidation pathways, (B) western blot results of lipophagy pathway, (C) the protein levels of lipophagy pathway, (D) western blot results of FFA β-oxidation pathway, (E) the protein levels of FFA β-oxidation pathway. The data are shown as the mean ± SEM. Values with different letters represent significant differences (p < 0.05)..
8). HIF-1α enhances autophagy to alleviate apoptosis in marginal cells in the stria vascular in neonatal rats under hypoxia. The International Journal of Biochemistry & Cell Biology, 2022 (PubMed: 35779841) [IF=3.4]
9). Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages. PLOS Neglected Tropical Diseases, 2022 (PubMed: 37141335) [IF=3.4]

Application: WB    Species: Human    Sample:

S1 Fig. Western blot analysis confirming Atg7 knockdown by siRNA. (A) Western blotting of Atg7 was conducted in THP-1 macrophages with or without knockdown treatment. (B) The ratio of Atg7/GAPDH was calculated. *P < 0.05, for the unpaired t-test. Representative images are displayed.
10). Autophagy activity and expression pattern of autophagy-related markers in the podocytes of patients with lupus nephritis: association with pathological classification. RENAL FAILURE, 2019 (PubMed: 31014158) [IF=3.0]

Application: IHC    Species: human    Sample: renal

Figure 4. |Representative findings on immunohistochemical staining of renal biopsy specimens from patients with LN with antibodies against Beclin1, microtubule-associated protein light chain 3 (LC3), and UNC-51-like kinase 1 (ULK1) autophagy-related gene 7 (Atg7). The arrows pointed to the positive signals.
加载更多

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.