C-RAF Antibody - #AF6065

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产品: C-RAF 抗体
货号: AF6065
描述: Rabbit polyclonal antibody to C-RAF
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P04049
RRID: AB_2834978

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
C-RAF Antibody detects endogenous levels of total C-RAF.
RRID:
AB_2834978
引用格式: Affinity Biosciences Cat# AF6065, RRID:AB_2834978.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

c Raf; C-raf; C-Raf proto-oncogene, serine/threonine kinase; CMD1NN; Craf 1 transforming gene; cRaf; Craf1 transforming gene; EC 2.7.11.1; kinase Raf1; Murine sarcoma 3611 oncogene 1; NS5; Oncogene MIL; Oncogene RAF1; OTTHUMP00000160218; OTTHUMP00000207813; OTTHUMP00000209389; Protein kinase raf 1; Proto-oncogene c-RAF; Raf 1; Raf 1 proto oncogene serine/threonine kinase; RAF; Raf proto oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF-1; RAF1; RAF1_HUMAN; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1; TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV; v raf 1 murine leukemia viral oncogene homolog 1; v-raf murine sarcoma viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine leukemia viral oncogene homolog 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human C-RAF, corresponding to a region within the internal amino acids.

基因/基因ID:
RAF1(5894)
描述:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Calothrixin B derivatives induce apoptosis and cell cycle arrest on HEL cells through the ERK/Ras/Raf/MEK pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38278023) [IF=6.9]
2). Platinum-Based Modification of Styrylbenzylsulfones as Multifunctional Antitumor Agents: Targeting the RAS/RAF Pathway, Enhancing Antitumor Activity, and Overcoming Multidrug Resistance. JOURNAL OF MEDICINAL CHEMISTRY, 2019 (PubMed: 31820986) [IF=6.8]

Application: WB    Species: Human    Sample: A549 cells

Figure 4. Compound 29 interfered with the interaction of Ras/Raf and MEK/ERK activation. (A) Cell lysates collected from A549 cells that were pretreated with 29 (5 μM) and 26 (5 μM) for 24 h were subjected to immunoprecipitation with anti-Ras antibodies and determined the associated CRAF with Western blot. (B) Statistics of gray intensity were normalized with input. Data were presented as the mean ± SD of three independent experiments. (C): MST trace lines of 29 binding to CRAF-RBD protein. (D): MST results of 29 binding to CRAF protein. Results provided above showed that 29 binds to the CRAF protein (Kd values was 149 nM). (E) Thermal stability of RAS; (F) statistics of gray intensity were normalized with gray intensity of 47 °C. Data were presented as the mean ± SD of three independent experiments.
3). Exercise-induced endothelial Mecp2 lactylation suppresses atherosclerosis via the Ereg/MAPK signalling pathway. Atherosclerosis, 2023 (PubMed: 37245426) [IF=4.9]
4). EphA10 drives tumor progression and immune evasion by regulating the MAPK/ERK cascade in lung adenocarcinoma. International Immunopharmacology, 2022 (PubMed: 35839564) [IF=4.8]
5). Design, Synthesis, and Anti-Leukemic Evaluation of a Series of Dianilinopyrimidines by Regulating the Ras/Raf/MEK/ERK and STAT3/c-Myc Pathways. Molecules (Basel, Switzerland), 2024 (PubMed: 38611876) [IF=4.6]

Application: IF/ICC    Species: Human    Sample: HEL cells

Figure 4 The effects of H-120 on the mitochondrial apoptotic and MAPK signaling pathways. (A) The expressions of caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, PARP, cleaved PARP, Bcl-xL, Bcl-2, and Bad were measured by Western blotting. (B,C): Quantification of relative protein expression levels of caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, PARP, cleaved PARP, Bcl-xL, Bid, Bcl-2, and Bad, with β-actin used as loading control. (D) The expressions of Ras, MEK, p-MEK, ERK, and p-MRK were measured by Western blotting (E,F): Quantification of relative protein expression levels of Ras, MEK, p-MEK, ERK, and p-MRK, with β-actin used as loading control. Data are represented as mean ± SD (n = 3, * p < 0.05, ** p < 0.01, *** p
6). Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. Journal of Cancer, 2023 (PubMed: 35281855) [IF=3.3]

Application: WB    Species: Mice    Sample: AGS cells

Fig 4 HRC induces GC cell EMT via Ca2+/CaM signaling. (A) AGS cells were transfected with control-siRNA or HRC-siRNA and stained with Fluo‑4/AM. (B) Knockdown of HRC reduced the intracellular Ca2+ concentration in AGS cells. (C) MKN-45 cells were transfected with control-siRNA or HRC-siRNA and stained with Fluo‑4/AM. (D) Knockdown of HRC reduced the intracellular Ca2+ concentration in MKN-45 cells. (E-H) Levels of p-c-Raf, c-Raf, p-MEK, MEK, p-ERK, ERK, E-cadherin, N-cadherin, Vimentin, Snail and Slug proteins in AGS and MKN-45 cells were analyzed by western blot. HRC, histidine-rich calcium binding protein; siRNA, small interfering RNA; c-Raf, Raf-1 proto-oncogene, serine/threonine kinase; p-c-Raf, phosphorylated c-Raf; MEK, MAPK/ERK kinase 1; p-MEK, phosphorylated MEK; ERK, extracellular regulated kinase; p-ERK, phosphorylated ERK; CaM, calmodulin. Data are shown as the mean ± SD; ns: no significant difference; *P < 0.05, **P < 0.01, ***P < 0.001, based on Student's t test.
7). Traditional Chinese Medicine CFF-1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR-related pathways in prostate cancer. Cancer Medicine, 2018 (PubMed: 29533017) [IF=2.9]
8). Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway. PloS one, 2025 (PubMed: 39879230) [IF=2.9]
9). Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017 (PubMed: 28743501) [IF=2.5]

Application: WB    Species: human    Sample:

Fig.2 Protein Expression of NDRD3-Raf-ERK pathway, A2a receptors, Cytochrome C.

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