IKK alpha/ beta Antibody - #AF6014

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产品: IKK alpha/ beta 抗体
货号: AF6014
描述: Rabbit polyclonal antibody to IKK alpha/ beta
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O15111 | O14920
RRID: AB_2834948

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IKK alpha/ beta Antibody detects endogenous levels of total IKK alpha/ beta.
RRID:
AB_2834948
引用格式: Affinity Biosciences Cat# AF6014, RRID:AB_2834948.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

chuk; CHUK1; Conserved Helix Loop Helix Ubiquitous Kinase; Conserved helix loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I Kappa B Kinase 1; I Kappa B Kinase Alpha; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK 1; IKK A; IKK a kinase; IKK-A; IKK-alpha; IKK1; IKKA; IKKA_HUMAN; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear Factor Kappa B Inhibitor Kinase Alpha; Nuclear factor NF kappa B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor; TCF-16; TCF16; Transcription factor 16; I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; IMD15; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta;

抗原和靶标

免疫原:

A synthesized peptide derived from human IKK alpha/ beta, corresponding to a region within the internal amino acids.

基因/基因ID:
CHUK(1147) | IKBKB(3551)
描述:
IKK-beta a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biology, 2022 (PubMed: 35500533) [IF=10.7]
2). A Novel pH-Responsive Baicalein@Chitosan Hydrogel for the Topical Treatment of Herpes Simplex Virus Type 1 Skin Infections: Therapeutic Potential and Mechanisms. Advanced healthcare materials, 2025 (PubMed: 40109148) [IF=10.0]
3). Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from Ganoderma leucocontextum fruiting bodies in vitro and in vivo. Food Chemistry, 2022 (PubMed: 35571333) [IF=8.5]

Application: WB    Species: mouse    Sample: RAW264.7 macrophages

Fig. 4. |Effects of GLP-1 on the expression levels of p-IKKα/β, p-IκBα, IκBα, p-NF-κB p65, and cytoplasmic and nuclear NF-κB p65 in RAW264.7 macrophages (A). Effects of GLP-1 on the expression levels of p-JNK, p-ERK, p-p38, and p-Akt in RAW264.7 macrophages (B).
4). Lactate facilitated mitochondrial fission-derived ROS to promote pulmonary fibrosis via ERK/DRP-1 signaling. Journal of translational medicine, 2024 (PubMed: 38773615) [IF=7.4]

Application: WB    Species: Mouse    Sample:

Fig. 5 Lactate promoted nuclear translocation of P65 through ROS and contributed to the development of pulmonary fibrosis. A Western blotting was performed for expressions determination of p65 in total fraction, cytoplasm fraction and nucleus fraction of MRC5. B p65 nuclear translocation assessed by immunofluorescence staining in HMCC97H cells with control group, lactate group, lactate + MT group and lactate + DPI group. C phosphorylation of NK-κB signaling components in MRC5 with control group, lactate group, lactate + MT group was determined by Immunoblotting. D, E COL1A1 and α-SMA was tested by Western blotting through SH-P65. *p 
5). BMP8B Activates Both SMAD2/3 and NF-κB Signals to Inhibit the Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes. Nutrients, 2023 (PubMed: 38201894) [IF=5.9]
6). Bmp8a deletion leads to obesity through regulation of lipid metabolism and adipocyte differentiation. Communications Biology, 2023 (PubMed: 37553521) [IF=5.9]

Application: WB    Species: Mouse    Sample: 3T3-L1 cells

Fig. 7 The interaction of NF-ĸB and PPARγ mediates the effect of Bmp8a on adipogenesis. a, c After induction of adipogenic differentiation, the downregulated (a) and upregulated (c) KEGG pathway in overexpression zebrafish bmp8a 3T3-L1 cells. b, d After induction of adipogenic differentiation, the downregulated (b) and upregulated (d) KEGG pathway in overexpression mouse Bmp8a 3T3-L1 cells. e, f Immunoblot analysis and quantification of p-IKKα/β and p-p65 in Mock, LV-ZsGreen1, and LV-bmp8a 3T3-L1 cells (n = 3). g, h Immunoblot analysis and quantification of p-IKKα/β and p-p65 in Mock, LV-ZsGreen1, and LV-Bmp8a 3T3-L1cells. Protein expression levels were quantified by ImageJ software and normalized to total protein (n = 3). i Co-immunoprecipitation and immunoblot analysis of co-transfected with PPARγ and p65 (n = 3). j Schematic drawing of predicted PPRE site in Fabp4 promoter region. k Schematic drawing of WT and PPRE site mutation Luc-report plasmids. l, m Quantification of the activity of Fabp4-promoter (l) and Fabp4-promoter-ΔPPRE (m) luciferase reporters in mouse HEK293T cells transfected with Vector, pCMV-Pparγ, or co-transfected pCMV-Pparγ and pCMV-p65, respectively. Renilla luciferase was used as the internal control (n = 3). Data were from three independent experiments and were analyzed by One-way ANOVA and were presented as mean ± SD
7). BMP8B Activates Both SMAD2/3 and NF-κB Signals to Inhibit the Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes. Nutrients, 2023 (PubMed: 38201894) [IF=5.9]
8). Targeting Parkin-regulated metabolomic change in cartilage in the treatment of osteoarthritis. iScience, 2024 (PubMed: 39220257) [IF=5.8]

Application: WB    Species: Mouse    Sample:

Figure 6. LLC inhibited NF-κB pathway and alleviated cartilage deterioration See also Figure S5. (A) GSEA analysis revealed that chemokine related pathways were enriched in aged cartilage while de-enriched in Prkn-KO cartilage. (B) mRNA level of genes associated with cartilage matrix, inflammation and matrix degrading enzymes of chondrocytes treated with LLC (n = 3). (C and D) Protein expression and (D) quantitative results of matrix degrading enzymes of chondrocytes treated with LLC (n = 3). (E and F) A representative image of immunohistochemistry staining and (F) quantitative results of the LLC-treated OA mouse model 4 weeks postoperatively (n = 3). Scale bar: 100 μm. (G and H) Protein expression and (H) quantitative results of IκBα, p-IKKα/β and IKKα/β of chondrocytes treated with LLC (n = 3). (I and J) Cytoplasmic and nuclear protein expression and (J) quantitative results of p65 of chondrocytes treated with LLC (n = 3). Statistical analysis was performed by two-tailed Student’s t test for comparisons of two groups.
9). Loganin alleviates sepsis-induced acute lung injury by regulating macrophage polarization and inhibiting NLRP3 inflammasome activation. International Immunopharmacology, 2021 (PubMed: 33744777) [IF=4.8]

Application: WB    Species: mice    Sample: Raw 264.7 cells

Fig. 6. Macrophage polarization induced by loganin maybe associated with the ERK and NF-κB pathway. Raw 264.7 cells were subjected to 5 μM, 10 μM, 20 μM loganin or 10 μM SCH772984 (an ERK inhibitor) or 10 μM JSH-23 (an NF-κB inhibitor) in the presence of 100 ng/ml LPS for 48 h. (A, B) The mRNA and protein levels of CD86 and iNOS (M1-macrophages markers) in LPSactivated Raw 264.7 cells were evaluated by RT-PCR and western blotting. (C, D) The mRNA and protein levels of CD206 and Arg-1 (M2-macrophages markers) in LPS-activated Raw 264.7 cells were assessed by RT-PCR and western blotting. Data were shown as means ± SD. n = 3. *p < 0.05 vs. control. #p < 0.05 vs. LPS. ERK, extracellular signal-regulated kinase; NF-κB, nuclear factor-kappa B; iNOS, inducible NO synthase; Arg-1, arginase-1; LPS, lipopolysaccharide.
10). Effects of glutamine on the IKK/IκB/NF-кB system in the enterocytes of turbot Scophthalmus maximus L. stimulated with soya-saponins. Fish & Shellfish Immunology, 2021 (PubMed: 34688862) [IF=4.1]
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