产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6009, RRID:AB_2834943.
展开/折叠
I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; IMD15; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta;
抗原和靶标
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
- O14920 IKKB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14920 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S4 | Phosphorylation | Uniprot | |
S6 | Phosphorylation | Uniprot | |
T23 | Phosphorylation | Uniprot | |
K44 | Ubiquitination | Uniprot | |
K106 | Ubiquitination | Uniprot | |
K147 | Ubiquitination | Uniprot | |
K163 | Ubiquitination | Uniprot | |
Y169 | Phosphorylation | Uniprot | |
K171 | Ubiquitination | Uniprot | |
S177 | Phosphorylation | P54646 (PRKAA2) , PR:000035736 (hMAP3K7/Phos:1) , P17252 (PRKCA) , Q02156 (PRKCE) , O43318 (MAP3K7) , Q99558 (MAP3K14) , Q05513 (PRKCZ) , Q9UHD2 (TBK1) | Uniprot |
C179 | S-Nitrosylation | Uniprot | |
T180 | Acetylation | Uniprot | |
T180 | Phosphorylation | Uniprot | |
S181 | Phosphorylation | Q9UHD2 (TBK1) , P17252 (PRKCA) , O00141 (SGK1) , O15530 (PDPK1) , Q99558 (MAP3K14) , P54646 (PRKAA2) , Q9Y6K9 (IKBKG) , PR:000035736 (hMAP3K7/Phos:1) , O14920 (IKBKB) , O43318 (MAP3K7) , Q05513 (PRKCZ) | Uniprot |
Y188 | Phosphorylation | P12931 (SRC) , P07948 (LYN) | Uniprot |
Y199 | Phosphorylation | P12931 (SRC) , P07948 (LYN) | Uniprot |
S239 | Phosphorylation | Uniprot | |
S246 | Phosphorylation | Uniprot | |
S256 | Phosphorylation | Uniprot | |
S257 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
Y261 | Phosphorylation | Uniprot | |
S267 | Phosphorylation | Uniprot | |
K301 | Ubiquitination | Uniprot | |
T324 | Phosphorylation | Uniprot | |
Y325 | Phosphorylation | Uniprot | |
S332 | Phosphorylation | Uniprot | |
S335 | Phosphorylation | Uniprot | |
K337 | Ubiquitination | Uniprot | |
T344 | Phosphorylation | Uniprot | |
K365 | Ubiquitination | Uniprot | |
T368 | Phosphorylation | Uniprot | |
S393 | Phosphorylation | Uniprot | |
T396 | Phosphorylation | Uniprot | |
Y397 | Phosphorylation | Uniprot | |
T399 | Phosphorylation | Uniprot | |
S402 | Phosphorylation | Uniprot | |
S409 | Phosphorylation | Uniprot | |
S411 | Phosphorylation | Uniprot | |
K418 | Ubiquitination | Uniprot | |
K467 | Ubiquitination | Uniprot | |
S471 | Phosphorylation | Uniprot | |
S474 | Phosphorylation | Uniprot | |
S476 | Phosphorylation | Uniprot | |
T488 | Phosphorylation | Uniprot | |
S489 | Phosphorylation | Uniprot | |
S507 | Phosphorylation | Uniprot | |
K509 | Ubiquitination | Uniprot | |
S550 | Phosphorylation | Uniprot | |
K555 | Ubiquitination | Uniprot | |
T559 | Phosphorylation | Uniprot | |
T583 | Phosphorylation | Uniprot | |
S587 | Phosphorylation | Uniprot | |
S600 | Phosphorylation | Uniprot | |
K603 | Ubiquitination | Uniprot | |
Y609 | Phosphorylation | Uniprot | |
T610 | Phosphorylation | Uniprot | |
K628 | Ubiquitination | Uniprot | |
S634 | Phosphorylation | Uniprot | |
K652 | Ubiquitination | Uniprot | |
K659 | Acetylation | Uniprot | |
K664 | Ubiquitination | Uniprot | |
S670 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S672 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S675 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S679 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S682 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S689 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S692 | Phosphorylation | O14920 (IKBKB) | Uniprot |
T693 | Phosphorylation | Uniprot | |
S695 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S697 | Phosphorylation | O14920 (IKBKB) | Uniprot |
K703 | Ubiquitination | Uniprot | |
S705 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S733 | O-Glycosylation | Uniprot | |
S733 | Phosphorylation | O14920 (IKBKB) , P53350 (PLK1) | Uniprot |
T735 | Phosphorylation | Uniprot | |
S740 | Phosphorylation | O14920 (IKBKB) , P53350 (PLK1) | Uniprot |
S750 | Phosphorylation | O14920 (IKBKB) , P53350 (PLK1) | Uniprot |
S756 | Phosphorylation | O14920 (IKBKB) | Uniprot |
翻译修饰 - O14920 作为激酶
Substrate | Site | Source |
---|---|---|
O14920-1 (IKBKB) | C179 | Uniprot |
O14920 (IKBKB) | S181 | Uniprot |
O14920 (IKBKB) | S670 | Uniprot |
O14920 (IKBKB) | S672 | Uniprot |
O14920 (IKBKB) | S675 | Uniprot |
O14920-1 (IKBKB) | S679 | Uniprot |
O14920 (IKBKB) | S682 | Uniprot |
O14920 (IKBKB) | S689 | Uniprot |
O14920-1 (IKBKB) | S692 | Uniprot |
O14920 (IKBKB) | S695 | Uniprot |
O14920-1 (IKBKB) | S697 | Uniprot |
O14920-1 (IKBKB) | S705 | Uniprot |
O14920-1 (IKBKB) | S733 | Uniprot |
O14920 (IKBKB) | S740 | Uniprot |
O14920-1 (IKBKB) | S750 | Uniprot |
O14920 (IKBKB) | S756 | Uniprot |
O43524 (FOXO3) | S644 | Uniprot |
O95999 (BCL10) | S134 | Uniprot |
P01100 (FOS) | S308 | Uniprot |
P04637 (TP53) | S362 | Uniprot |
P04637 (TP53) | S366 | Uniprot |
P19438 (TNFRSF1A) | S381 | Uniprot |
P19838 (NFKB1) | S923 | Uniprot |
P19838-2 (NFKB1) | S924 | Uniprot |
P19838 (NFKB1) | S927 | Uniprot |
P19838-2 (NFKB1) | S928 | Uniprot |
P19838 (NFKB1) | S932 | Uniprot |
P23396 (RPS3) | S209 | Uniprot |
P25963 (NFKBIA) | S32 | Uniprot |
P25963 (NFKBIA) | S36 | Uniprot |
P31946 (YWHAB) | S132 | Uniprot |
P35568 (IRS1) | S268 | Uniprot |
P35568 (IRS1) | S270 | Uniprot |
P35568 (IRS1) | S272 | Uniprot |
P35568 (IRS1) | S274 | Uniprot |
P35568 (IRS1) | S307 | Uniprot |
P35568 (IRS1) | S312 | Uniprot |
P35568 (IRS1) | S341 | Uniprot |
P35568 (IRS1) | S345 | Uniprot |
P35568 (IRS1) | S527 | Uniprot |
P35568 (IRS1) | S531 | Uniprot |
P42771 (CDKN2A) | S8 | Uniprot |
Q01201 (RELB) | S472 | Uniprot |
Q04206 (RELA) | S468 | Uniprot |
Q04206 (RELA) | S536 | Uniprot |
Q04864 (REL) | S557 | Uniprot |
Q13568 (IRF5) | S446 | Uniprot |
Q14164 (IKBKE) | S172 | Uniprot |
Q14653 (IRF3) | S396 | Uniprot |
Q14653 (IRF3) | S398 | Uniprot |
Q14653 (IRF3) | S402 | Uniprot |
Q14653 (IRF3) | T404 | Uniprot |
Q14653 (IRF3) | S405 | Uniprot |
Q15653 (NFKBIB) | S19 | Uniprot |
Q15653-1 (NFKBIB) | S23 | Uniprot |
Q16875 (PFKFB3) | S269 | Uniprot |
Q5S007 (LRRK2) | S910 | Uniprot |
Q5S007 (LRRK2) | S935 | Uniprot |
Q92574 (TSC1) | S487 | Uniprot |
Q92574 (TSC1) | S511 | Uniprot |
Q96CV9 (OPTN) | S513 | Uniprot |
Q99704 (DOK1) | S439 | Uniprot |
Q99704 (DOK1) | S443 | Uniprot |
Q99704 (DOK1) | S446 | Uniprot |
Q99704 (DOK1) | S450 | Uniprot |
Q9BXH1 (BBC3) | S10 | Uniprot |
Q9H3D4 (TP63) | S43 | Uniprot |
Q9H3D4 (TP63) | S51 | Uniprot |
Q9NQC7 (CYLD) | S418 | Uniprot |
Q9NQC7 (CYLD) | S422 | Uniprot |
Q9NQC7 (CYLD) | S432 | Uniprot |
Q9NQC7 (CYLD) | S436 | Uniprot |
Q9NQC7 (CYLD) | S439 | Uniprot |
Q9NQC7 (CYLD) | S441 | Uniprot |
Q9NQC7 (CYLD) | S444 | Uniprot |
Q9Y4G8 (RAPGEF2) | S1254 | Uniprot |
Q9Y6K9-1 (IKBKG) | S31 | Uniprot |
Q9Y6K9 (IKBKG) | S43 | Uniprot |
Q9Y6K9 (IKBKG) | S68 | Uniprot |
Q9Y6K9 (IKBKG) | S85 | Uniprot |
Q9Y6K9-1 (IKBKG) | S376 | Uniprot |
Q9Y6Q9-5 (NCOA3) | S857 | Uniprot |
研究背景
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity).
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B.
(Microbial infection) Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to 'Ser-163' does not serve as a monoubiquitination site. According to ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.
(Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia yopJ.
Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B.
Cytoplasm. Nucleus. Membrane raft.
Note: Colocalized with DPP4 in membrane rafts.
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65 (By similarity). Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6 (By similarity). May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with TRIM21. Interacts with NLRC5; prevents IKBKB phosphorylation and kinase activity. Interacts with PDPK1. Interacts with EIF2AK2/PKR. The phosphorylated form interacts with PPM1A and PPM1B. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner. Interacts with IKBKE. Interacts with NAA10, leading to NAA10 degradation. Interacts with FOXO3. Interacts with AKAP13. Interacts with IFIT5; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation. Interacts with LRRC14; disrupts IKBKB-IKBKG interaction preventing I-kappa-B-kinase (IKK) core complex formation and leading to a decrease of IKBKB phosphorylation and NF-kappaB activation. Interacts with SASH1. Interacts with ARFIP2.
(Microbial infection) Interacts with Yersinia yopJ.
(Microbial infection) Interacts with vaccinia virus protein B14.
The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
文献引用
Application: WB Species: Mouse Sample: HT22 cells
Application: WB Species: mice Sample: adipose tissues
Application: WB Species: rat Sample: hippocampus
Application: WB Species: mouse Sample: colorectal
Application: WB Species: Rat Sample: cortex
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.