AFfirm™ ERK1/2 Mouse monoclonal Antibody - #BF8004

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产品: ERK1/2 小鼠 单克隆 抗体
货号: BF8004
描述: Mouse monoclonal antibody to ERK1/2
应用: WB IHC
文献验证: WB
反应: Mouse, Rat
蛋白号: P27361 | P28482
RRID: AB_2846228

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   规格 价格 库存
 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

货期: 当天发货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Mouse, Rat
克隆:
Monoclonal [AFfirm8004(AFB17821)]
特异性:
p44/42 MAPK (Erk1/2) antibody detects endogenous levels of total p44/42 MAPK (Erk1/2).
RRID:
AB_2846228
引用格式: Affinity Biosciences Cat# BF8004, RRID:AB_2846228.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ERK 1; ERK; ERK-1; ERK1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3; ERK 2; ERK; ERK-2; ERT1; Extracellular Signal Regulated Kinase 2; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; Mapk1; MAPK2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; P38; P40; P41; p42-MAPK; P42MAPK; PRKM1; PRKM2; protein kinase, mitogen-activated, 1; protein kinase, mitogen-activated, 2; protein tyrosine kinase ERK2;

抗原和靶标

免疫原:

A synthesized peptide derived from human ERK1/2

基因/基因ID:
MAPK3(5595) | MAPK1(5594)
描述:
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). WLJP-025p, a homogeneous Lonicera japonica polysaccharide, attenuates atopic dermatitis by regulating the MAPK/NFκB/AP-1 axis via Act1. International journal of biological macromolecules, 2024 (PubMed: 38016605) [IF=7.7]
2). Macrophage-derived exosomal HMGB3 regulates silica-induced pulmonary inflammation by promoting M1 macrophage polarization and recruitment. Particle and fibre toxicology, 2024 (PubMed: 38454505) [IF=7.2]

Application: WB    Species: Mouse    Sample: RAW264.7 macrophages

Fig. 5 SiO2-Exo promotes the inflammatory response by regulating the activation of the STAT3/MAPK (ERK1/2 and p38)/NF-κB signalling pathways. (A) Western blot analysis of the expression of pro-IL-1β and the phosphorylation of p65 (NF-κB), STAT1/3, AKT, ERK1/2 and p38 in RAW264.7 macrophages treated with PBS, NC-Exo or SiO2-Exo. # indicates that the data were compared between the NC-Exo group and the SiO2-Exo group. (B) Western blot analysis of the expression of pro-IL-1β and the phosphorylation levels of p65 (NF-κB), STAT1/3, AKT, ERK1/2 and p38 in RAW264.7 macrophages treated with SiO2-Exo or SiO2 + GW4869-Exo. (C) Western blot analysis of the expression of pro-IL-1β and the phosphorylation of STAT3 and AKT in SiO2-Exo-induced RAW264.7 macrophages treated with Stattic (5 µM) or MK2206 (10 nM). (D) ELISA analysis of the release of IL-1β, IL-6 and TNF-α in the SN of SiO2-Exo-induced RAW264.7 macrophages treated with Stattic (5 µM) or MK2206 (10 nM). n = 3 each group. The data are representative of three individual experiments and expressed as the mean ± SEM. The data were analysed by Student’s t test or two-way ANOVA. *P 
3). Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38325261) [IF=6.9]

Application: WB    Species: Human    Sample: HVSMCs

Fig. 5. Artesunate reduces the protein phosphorylation of MAPKs/NF-κB/AP-1 pathway and is associated with NLRP3. (A) Relative protein expression of p-ERK1/2, p-JNK, and p-p38 in vitro. (B) Relative mRNA expression levels of MAPK1, MAPK8, and MAPK14 in vitro. (C) Relative protein expression of p-c-Fos and p-p65 in vitro. (D) Relative mRNA expression levels of FOS, JUN, and NFKB1A in vitro. *P 
4). Corynoxine exerts the anti-tumor effect on esophageal squamous cell carcinoma principally via the EZH2-DUSP5-ERK1/2-mediated cell growth inhibition. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 39383633) [IF=6.7]
5). Deletion of Meg8-DMR Enhances Migration and Invasion of MLTC-1 Depending on the CTCF Binding Sites. International Journal of Molecular Sciences, 2022 (PubMed: 35955961) [IF=5.6]

Application: WB    Species: Mouse    Sample:

Figure 6. Overexpression of Dlk1 in Meg8−DMR−KO suppressed cells’ migration and invasion by blocking Notch1−Rhoc−MAPK/ERK. (A) Western blot shows the expression level of Dlk1 was reduced while Nocth1 Rhoc and p-ERK1/2 were activated in Meg8-DMR-KO. When the overexpression vector pcDNA3.1-Dlk1 was transfected into Meg8-DMR-KO, the expression of Nocth1, Rhoc, and p-ERK1/2 was reduced. (B) Immunofluorescence staining of Phalloidin-California Red Conjugate in WT, Meg8-DMR-KO, and Meg8-DMR-KO with Dlk1 overexpression. (C) Confluent monolayers of cells of WT, Meg8-DMR-KO and Meg8-DMR-KO with Dlk1 overexpression were wounded and after being incubated for an additional 48 h, the relative migration ratio was calculated. (D) Migration and invasion of WT, Meg8-DMR-KO, and Meg8-DMR-KO with Dlk1 overexpression were analyzed. (E) Cell proliferation of WT, Meg8-DMR-KO and Meg8-DMR-KO with Dlk1 overexpression was measured via MTT assay. (F) Colonies grown from cells of WT, Meg8-DMR-KO, and Meg8-DMR-KO with Dlk1 overexpression were counted. Error bars, mean ± SEM. n ≥ 3. p values were calculated using t-test. * p < 0.05, ** p < 0.01, ns (non-significant), scale bars = 200 μm.
6). α9 Nicotinic Acetylcholine Receptor Promotes Tumor Proliferation and Suppresses Ferroptosis in Triple-Negative Breast Cancer. Biomolecules, 2025 (PubMed: 40563476) [IF=5.5]
7). In vitro anti-Helicobacter pylori activity of Syzygium aromaticum and the preliminary mechanism of action. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35032584) [IF=4.8]
8). Profilin1 Regulates Trophoblast Invasion and Macrophage Differentiation. The American Journal of Pathology, 2023 (PubMed: 37164274) [IF=4.7]
9). Novel circRNA_0071196/miRNA‑19b‑3p/CIT axis is associated with proliferation and migration of bladder cancer. INTERNATIONAL JOURNAL OF ONCOLOGY, 2020 (PubMed: 32705161) [IF=4.5]
10). SPHK1/S1P/S1PR pathway promotes the progression of peritoneal fibrosis by mesothelial-mesenchymal transition. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024 (PubMed: 38226856) [IF=4.4]
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