Phospho-p38 MAPK (Tyr182) Antibody - #AF3455

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产品: 磷酸化 p38 MAPK (Tyr182) 抗体
货号: AF3455
描述: Rabbit polyclonal antibody to Phospho-p38 MAPK (Tyr182)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q16539
RRID: AB_2834893

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-p38 MAPK (Tyr182) Antibody detects endogenous levels of p38 MAPK only when phosphorylated at Tyrosine 182.
RRID:
AB_2834893
引用格式: Affinity Biosciences Cat# AF3455, RRID:AB_2834893.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CSAID Binding Protein 1; CSAID binding protein; CSAID-binding protein; Csaids binding protein; CSBP 1; CSBP 2; CSBP; CSBP1; CSBP2; CSPB1; Cytokine suppressive anti-inflammatory drug-binding protein; EXIP; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX interacting protein 2; MAX-interacting protein 2; Mitogen Activated Protein Kinase 14; Mitogen activated protein kinase p38 alpha; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14_HUMAN; Mxi 2; MXI2; p38 ALPHA; p38; p38 MAP kinase; p38 MAPK; p38 mitogen activated protein kinase; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK2A;

抗原和靶标

免疫原:

A synthesized peptide derived from human p38 MAPK around the phosphorylation site of Tyr182.

基因/基因ID:
MAPK14(1432)
描述:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Modified Guo-Min decoction ameliorates PM2.5-induced lung injury by inhibition of PI3K-AKT and MAPK signaling pathways. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38061286) [IF=6.7]
2). The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways. PHYTOMEDICINE, 2019 (PubMed: 30878874) [IF=6.7]
3). A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway. Marine Drugs, 2022 (PubMed: 35200639) [IF=5.4]
4). Anti-inflammatory action of physalin A by blocking the activation of NF-κB signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2021 (PubMed: 33091501) [IF=4.8]

Application: WB    Species: Mice    Sample: RAW 264.7 cells

Figure 7. Effect of physalin A on the phosphorylation of ERK (a, b), JNK (c, d) and p38 (e, f) proteins of the MAPKs pathway in LPS-activated RAW 264.7 cells. β-actin was used as internal reference to confirm the equal loading of proteins. Densitometric analysis data were standardized on the basis of levels of unphosphorylated forms (ERK, JNK, p38). The results of phosphorylated ERK protein, phosphorylated JNK protein, phosphorylated p38 protein are presented as mean ± S.E.M. from three independent experiments. ##P < 0.01 vs. untreated group, **P < 0.01 vs. LPS treatment group.
5). Repurposed drug agomelatine is therapeutic against collagen-induced arthritis via iNOS targeting. International immunopharmacology, 2024 (PubMed: 38442577) [IF=4.8]
6). Anti-inflammatory effects of three withanolides isolated from Physalis angulata L. in LPS-activated RAW 264.7 cells through blocking NF-κB signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2021 (PubMed: 33957208) [IF=4.8]

Application: WB    Species: Mice    Sample: RAW 264.7 cells

Fig. 7. Effect of physagulin A, physagulin C, physagulin H on the phosphorylation of ERK, JNK, and p38 proteins of the MAPK pathway in LPSactivated RAW 264.7 cells. The cells were treated by LPS (1 μg/mL) with or without physagulin A (0.78, 1.56, 3.12, 6.25 μM), physagulin C (0.39, 0.78, 1.56, 3.12 μM), physagulin H (1.56, 3.12, 6.25, 12.5 μM) for 1 h (detection of phosphorylated-JNK) or 15 min (detection of phosphorylated-ERK and phosphorylated-p38), and the expressions of p-ERK, p-JNK, p-p38 proteins were determined by Western blot analysis. Unphosphorylated form of ERK, JNK, p38 proteins were used as internal reference to confirm the equal loading of proteins. Densitometric analysis data were standardized on the basis of ERK, JNK, p38 protein levels. The results of phosphorylated JNK protein (a, b, c), phosphorylated ERK protein (d, e, f), phosphorylated p38 protein (g, h, i) are presented as mean ± S.E.M. from three independent experiments. ##P < 0.01 vs. untreated group, **P < 0.01 vs. LPS treatment group.
7). Polyethylene Terephthalate Microplastic Exposure Induced Reproductive Toxicity Through Oxidative Stress and p38 Signaling Pathway Activation in Male Mice. Toxics, 2024 (PubMed: 39590959) [IF=4.6]

Application: WB    Species: Human    Sample: testis

Figure 8. The activation of the p38 MAPK signaling pathway after PET MP exposure. (a) The expression of p38 and p-p38 in testis; (b) the quantification of p38 and p-p38 protein levels. β-actin was considered as the loading control. n = 5 for all groups. Data are presented as the mean ± SEM. * indicates p < 0.05 (compared with the control group). ** indicates p < 0.01 (compared with the control group).
8). (3R, 7R)-7-Acetoxyl-9-Oxo-de-O-Methyllasiodiplodin, a Secondary Metabolite of Penicillium Sp., Inhibits LPS-Mediated Inflammation in RAW 264.7 Macrophages through Blocking ERK/MAPKs and NF-κB Signaling Pathways. INFLAMMATION, 2019 (PubMed: 31011928) [IF=4.5]
9). Tripterygium glycoside ameliorates neuroinflammation in a mouse model of Aβ25-35-induced Alzheimer's disease by inhibiting the phosphorylation of IκBα and p38. Bioengineered, 2021 (PubMed: 34592905) [IF=4.2]

Application: WB    Species: Mice    Sample:

Figure 4. The expression levels of p-P38, P38, p-IκBα, Caspase1, COX2, and iNOS proteins between groups. Quantitative summaries of the protein levels relative to α-tubulin as an internal control, expressed as a percentage of α-tubulin optical density (o.d.) for the groups (n = 8/group). Statistical results (Kruskal–Wallis nonparametric test with Dunn’s multiple post hoc comparison) were shown in the bar graphs, with ‘**’ indicating significant inter-group difference. Data was expressed as the mean ± standard error of the mean (SEM)(n = 8/group). (a–g) The expression levels of p-P38, P38, p-IκBα, Caspase1, COX2, and iNOS proteins in AD+TG, AD+donepezil, AD mice model and Normal control groups. (a) p-P38; (b) P38; (c) p-IκBα; (d) Caspase1; (e) COX2; (f) iNOS. (g) The western blotting results of p-P38, P38, p-IκBα, Caspase1, COX2, and iNOS proteins. (h–n) The expression levels of p-P38, P38, p-IκBα, Caspase1, COX2, and iNOS proteins in AD+TG, AD cell model and Normal control groups. (h) p-P38; (i) P38; (j) p-IκBα; (k) Caspase1; (l) COX2; (m) iNOS. (n) The western blotting results of p-P38, P38, p-IκBα, Caspase1, COX2, and iNOS proteins. ‘**’ indicating significant inter-group difference. TG: Tripterygium glycoside; AD: Alzheimer disease; NS: normal saline
10). Huang Lian Jie Du decoction attenuates inflammation in septic rats by activating autophagy and altering the intestinal microbiome. Heliyon, 2024 (PubMed: 38828290) [IF=4.0]
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