产品: 磷酸化 IRF3 (Ser386) 抗体
货号: AF3438
描述: Rabbit polyclonal antibody to Phospho-IRF3 (Ser386)
应用: WB IHC IF/ICC
反应: Human, Rat
预测: Pig, Bovine, Sheep, Dog
分子量: 57kDa; 47kD(Calculated).
蛋白号: Q14653
RRID: AB_2834880

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Rat
预测:
Pig(92%), Bovine(92%), Sheep(92%), Dog(85%)
克隆:
Polyclonal
特异性:
Phospho-IRF3 (Ser386) Antibody detects endogenous levels of IRF3 only when phosphorylated at Serine 386.
RRID:
AB_2834880
引用格式: Affinity Biosciences Cat# AF3438, RRID:AB_2834880.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IIAE7; Interferon regulatory factor 3; IRF 3; IRF-3; IRF3; IRF3_HUMAN; MGC94729;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q14653 IRF3_HUMAN:

Expressed constitutively in a variety of tissues.

描述:
IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP.
序列:
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
92
Bovine
92
Sheep
92
Dog
85
Horse
77
Chicken
75
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14653 作为底物

Site PTM Type Enzyme
T3 Phosphorylation
S14 Phosphorylation
K29 Ubiquitination
K70 Sumoylation
K70 Ubiquitination
T75 Phosphorylation
K77 Ubiquitination
K87 Sumoylation
K87 Ubiquitination
S97 Phosphorylation
S123 Phosphorylation
T135 Phosphorylation P78527 (PRKDC)
S173 Phosphorylation P45984 (MAPK9) , P45983 (MAPK8) , Q9UHD2 (TBK1)
S175 Phosphorylation Q9UHD2 (TBK1)
T180 Phosphorylation
S188 Phosphorylation
K193 Ubiquitination
T237 Phosphorylation
T244 Phosphorylation
T253 Phosphorylation
S259 Phosphorylation
K313 Ubiquitination
K315 Ubiquitination
S339 Phosphorylation
T370 Phosphorylation
S385 Phosphorylation Q9UHD2 (TBK1)
S386 Phosphorylation Q14164 (IKBKE) , Q9UQM7 (CAMK2A) , Q9UHD2 (TBK1)
T390 Phosphorylation
S396 Phosphorylation O14920 (IKBKB) , Q14164 (IKBKE) , Q9UHD2 (TBK1)
S398 Phosphorylation O14920 (IKBKB) , Q14164 (IKBKE) , Q9UHD2 (TBK1)
S402 Phosphorylation O14920 (IKBKB) , Q14164 (IKBKE) , Q9UHD2 (TBK1)
T404 Phosphorylation O14920 (IKBKB) , Q9UHD2 (TBK1)
S405 Phosphorylation O14920 (IKBKB) , Q9UHD2 (TBK1)
S427 Phosphorylation

研究背景

功能:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

翻译修饰:

Constitutively phosphorylated on many Ser/Thr residues. Activated following phosphorylation by TBK1 and IKBKE. Innate adapter protein MAVS, STING1 or TICAM1 are first activated by viral RNA, cytosolic DNA, and bacterial lipopolysaccharide (LPS), respectively, leading to activation of the kinases TBK1 and IKBKE. These kinases then phosphorylate the adapter proteins on the pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1. Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce IFNs.

(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.

Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.

ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

细胞定位:

Cytoplasm. Nucleus.
Note: Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757). When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed constitutively in a variety of tissues.

亚基结构:

Monomer. Homodimer; phosphorylation-induced. Interacts (when phosphorylated) with CREBBP. Interacts with MAVS (via phosphorylated pLxIS motif). Interacts with TICAM1 (via phosphorylated pLxIS motif). Interacts with STING1 (via phosphorylated pLxIS motif). Interacts with IKBKE and TBK1. Interacts with TICAM2. Interacts with RBCK1. Interacts with HERC5. Interacts with DDX3X (phosphorylated at 'Ser-102'); the interaction allows the phosphorylation and activation of IRF3 by IKBKE. Interacts with TRIM21 and ULK1, in the presence of TRIM21; this interaction leads to IRF3 degradation by autophagy. Interacts with RIOK3; RIOK3 probably mediates the interaction of TBK1 with IRF3. Interacts with ILRUN; the interaction inhibits IRF3 binding to its DNA consensus sequence. Interacts with LYAR; this interaction impairs IRF3 DNA-binding activity.

(Microbial infection) Interacts with rotavirus A NSP1 (via pLxIS motif); this interaction leads to the proteasome-dependent degradation of IRF3.

(Microbial infection) Interacts with herpes virus 8/HHV-8 protein VIRF1.

(Microbial infection) Interacts with Seneca Valley virus protease 3C; this interaction is involved in the suppression of IRF3 expression and phosphorylation by the virus.

蛋白家族:

Belongs to the IRF family.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). Inhibition of Stimulator of Interferon Genes Protects Against Myocardial Ischemia-Reperfusion Injury in Diabetic Mice. Cardiovascular Innovations and Applications, 2023 [IF=0.5]

Application: WB    Species: Mouse    Sample: Hearts

Figure 4 STING Knockout Mitigates the Inflammatory Response in Diabetic Mice Subjected to I/R Injury. Mice were treated as described in Figure 2. (A) Representative western blotting results of p-TBK, p-IRF3, and IL-1β. (B) Statistical analysis of p-TBK/TBK in each group. (C) Statistical analysis of p-IRF3/IRF3 in each group. (D) Statistical analysis of IL-1β/GAPDH in each group. (E) Immunohistochemical results of TNF-α and IL-1β. Data are expressed as means ± SD, n=3.

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