Phospho-NF-kB p65 (Ser311) Antibody - #AF3389

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产品: 磷酸化 NF-kB p65 (Ser311) 抗体
货号: AF3389
描述: Rabbit polyclonal antibody to Phospho-NF-kB p65 (Ser311)
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: Q04206
RRID: AB_2827387

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-NF-kB p65 (Ser311) Antibody detects endogenous levels of NF-kB p65 only when phosphorylated at Serine 311.
RRID:
AB_2827387
引用格式: Affinity Biosciences Cat# AF3389, RRID:AB_2827387.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NF kappa B p65delta3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; OTTHUMP00000233473; OTTHUMP00000233474; OTTHUMP00000233475; OTTHUMP00000233476; OTTHUMP00000233900; p65; p65 NF kappaB; p65 NFkB; relA; TF65_HUMAN; Transcription factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V rel avian reticuloendotheliosis viral oncogene homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65;

抗原和靶标

免疫原:

A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Ser311.

基因/基因ID:
RELA(5970)
描述:
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Extracellular vesicles derived from mesenchymal stem cells alleviate neuroinflammation and mechanical allodynia in interstitial cystitis rats by inhibiting NLRP3 inflammasome activation. Journal of Neuroinflammation, 2022 (PubMed: 35387668) [IF=9.3]

Application: WB    Species: rat    Sample: spinal dorsal horn

Fig. 8 | MSC-EVs inhibit activity of TLR4/NF-κB signal pathway in SDH of IC rats. A–C Western blot analysis showing that expression level of TLR4 and phosphorylation ratio of NF‐κB (p65) were signifcantly increased in SDH of IC rats compared with normal rats, and intrathecal injection of MSC-EVs signifcantly decreased expression level of TLR4 and phosphorylation ratio of NF‐κB (p65) in SDH of IC rats
2). Normalization of magnesium deficiency attenuated mechanical allodynia, depressive-like behaviors, and memory deficits associated with cyclophosphamideinduced cystitis by inhibiting TNF-α/NF-κB signaling in female rats. Journal of Neuroinflammation, 2020 (PubMed: 32241292) [IF=9.3]

Application: WB    Species: Rat    Sample: SDH

Fig. 4 TNF-α/NF-κB signaling and IL-1β were upregulated in the SDH and hippocampus of the CYP-induced cystitis model. a–c Western blot analysis results indicate that TNF-α, the phospho-p65 (p-p65)/p65 ratio, and IL-1β were increased in the SDH of the CYP-induced cystitis model at all three time points (days 8, 12, and 20) after CYP injection. d–f TNF-α, the p-p65/p65 ratio, and IL-1β also showed higher expression in the hippocampus of the cystitis group than in the Veh group at the three time points. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. Veh group. The data were analyzed by one-way ANOVA followed by the Tukey post hoc test

Application: IF/ICC    Species: Rat    Sample: SDH

Fig. 5 TNF-α, p-p65, and IL-1β all co-localize with NeuN in the SDH of CYP-induced cystitis rats. Photographs of the double immunofluorescence staining indicate that TNF-α, p-p65 (a principal transcriptional regulator of the activation of NF-kB pathway), and IL-1β were only co-localized with NeuN-labeled neurons but not with GFAP-labeled astrocytes or OX-42-labeled microglia in the SDH of cystitis animals
3). Mitigation of inflammatory bowel disease-related osteoporosis by oxyberberine: Insights into the RANKL/NF-κB signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38574627) [IF=6.9]
4). Chronic Oral Administration of Magnesium-L-Threonate Prevents Oxaliplatin-Induced Memory and Emotional Deficits by Normalization of TNF-α/NF-κB Signaling in Rats. Neuroscience Bulletin, 2021 (PubMed: 32857294) [IF=5.9]
5). Formononetin Protects LPS-Induced Mastitis Through Suppressing Inflammation and Enhancing Blood-Milk Barrier Integrity via AhR-Induced Src Inactivation. Frontiers in Immunology, 2022 (PubMed: 35185907) [IF=5.7]

Application: WB    Species: Mice    Sample: EpH4-Evs cells

Figure 9 FOR inhibits LPS-induced inflammation via AhR-induced Src inactivation. (A) The levels of proinflammatory cytokines TNF-α and IL-1β in EpH4-Evs cells. (B) The expression of NF-κB signaling pathway. (C) The expression of tight junction proteins occludin, claudin-3, and ZO-1 in EpH4-Evs cells. The present results are displayed as the mean ± SEM of triple parallel measurements. #(P < 0.01) is significantly different from control group; **(P < 0.01) is significantly different from LPS group.
6). The Causal Role of Magnesium Deficiency in the Neuroinflammation, Pain Hypersensitivity and Memory/Emotional Deficits in Ovariectomized and Aged Female Mice. Journal of Inflammation Research, 2023 (PubMed: 34908863) [IF=4.5]

Application: IF/ICC    Species: Mouse    Sample:

Figure 4 Oral application of L-TAMS attenuates the activation of NF-κB and upregulation of TNF-α and IL-1β in dorsal root ganglion (DRG) neurons of ovariectomized and aged mice. (A–C) Representative immunofluorescent staining images show the expressions of p-p65 (A), TNF-α (B) and IL-1β (C) in DRG neurons in indicated groups. Notes: p-p65 is mainly located in nuclei, while TNF-α and IL-1β in cytoplasm and nerve fibers. Scale bar: 100 μm. (D) Statistical analysis revealed p-p65, TNF-α and IL-1β in DRG neurons were upregulated in OVX and aged mice, and the changes were attenuated by oral L-TAMS (n = 3 mice/group, 2 sections/mouse). The data were analyzed by one-way ANOVA with Tukey’s test.

Application: WB    Species: Mouse    Sample:

Figure 4 Oral application of L-TAMS attenuates the activation of NF-κB and upregulation of TNF-α and IL-1β in dorsal root ganglion (DRG) neurons of ovariectomized and aged mice. (A–C) Representative immunofluorescent staining images show the expressions of p-p65 (A), TNF-α (B) and IL-1β (C) in DRG neurons in indicated groups. Notes: p-p65 is mainly located in nuclei, while TNF-α and IL-1β in cytoplasm and nerve fibers. Scale bar: 100 μm. (D) Statistical analysis revealed p-p65, TNF-α and IL-1β in DRG neurons were upregulated in OVX and aged mice, and the changes were attenuated by oral L-TAMS (n = 3 mice/group, 2 sections/mouse). The data were analyzed by one-way ANOVA with Tukey’s test.
7). Radioprotective effect of polyvinylpyrrolidone modified selenium nanoparticles and its antioxidation mechanism in vitro and in vivo. Frontiers in bioengineering and biotechnology, 2024 (PubMed: 38962664) [IF=4.3]

Application: WB    Species: Human    Sample: HUVECs

FIGURE 5. Western blot was performed to detect NF-κB and MAPK-associated proteins in HUVECs.
8). Umbilical Cord Mesenchymal Stem Cells Overexpressing Heme Oxygenase-1 Promotes Symptoms Recovery in Cystitis Rats by Alleviating Neuroinflammation. Stem cells international, 2023 (PubMed: 38020203) [IF=3.8]

Application: WB    Species: Rat    Sample:

Figure 8 Transduced hUMSCs inhibit activity of TLR4/p65/NLRP3 signal pathway in SDH region. (a) Immunofluorescence staining of NLRP3 (red), the arrows were pointing to NLRP3 positive cells. Scale bar = 50 μm. (b–e) Western blot analysis showing that expression level of TLR4, phosphorylation ratio of p65, and NLRP3 were significantly decreased in SDH after HO-1-hUMSCs treatment. All data were calculated as mean (±SEM) (n = 6 per group).  ∗ ∗p < 0.01,  ∗ ∗ ∗p < 0.001 vs. the CYP group, and #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. the hUMSC group.
9). IL-33-dependent NF-κB activation inhibits apoptosis and drives chemoresistance in acute myeloid leukemia. Cytokine, 2024 (PubMed: 38852492) [IF=3.7]
10). Effect of human epididymis protein 4 on hyperoxia-induced bronchial dysplasia in newborn rats. Nucleosides, nucleotides & nucleic acids, 2025 (PubMed: 39004907) [IF=1.1]
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