TNF Receptor I Antibody - #AF0282

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产品: TNF Receptor I 抗体
货号: AF0282
描述: Rabbit polyclonal antibody to TNF Receptor I
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
蛋白号: P19438
RRID: AB_2834164

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 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TNF Receptor I Antibody detects endogenous levels of total TNF Receptor I.
RRID:
AB_2834164
引用格式: Affinity Biosciences Cat# AF0282, RRID:AB_2834164.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD120a; FPF; MGC19588; p55; p55-R; p60; TBP1; TBPI; TNF R; TNF R55; TNF-R1; TNF-RI; TNFAR; TNFR-I; TNFR1; TNFR55; TNFR60; TNFRI; TNFRSF1a; TNR1A_HUMAN; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor superfamily, member 1A; Tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; Tumor necrosis factor-binding protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human TNFR1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
TNFRSF1A(7132)
描述:
TNF-R1 Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Neutrophil-Mimetic Upconversion Photosynthetic Nanosystem Derived from Microalgae for Targeted Treatment of Thromboembolic Stroke. ACS nano, 2024 (PubMed: 39465976) [IF=15.8]
2). Alpha-(1,6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling. Cell death & disease, 2021 (PubMed: 34857735) [IF=8.1]
3). 20 (S)-Ginsenoside Rh2 induces caspase-dependent PML-RARA degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades. Journal of Ginseng Research, 2021 (PubMed: 33841010) [IF=6.8]

Application: WB    Species: Human    Sample: NB4 cells

Fig. 7. GRh2 activated the TNF-a/caspase8 cascade. (A) NB4 cells were incubated with 30 mM, 40 mM, or 50 mM GRh2 for 12 h. Protein expression levels of FasL, Fas, TNF-a, and TNFR1 in NB4 cells were detected via Western blot. A representative picture of three replicates is shown. (B) Quantitative statistical graph of the relative protein expression levels. The results are shown as the mean  SD (n ¼ 3) *p < 0.05, **p < 0.01. (C) RT-PCR was used to detect the mRNA expression level of TNF-a in NB4 cells after GRh2 administration. The results are shown as the mean  SD (n ¼ 3) **p < 0.01, ***p < 0.001 versus solvent. After preincubation with 1.5 mM TNF-a inhibitor, C 87, for 2 h, 30 mM, 40 mM, or 50 mM GRh2 was applied for another 12 h. (D) CCK-8 assay measured the NB4 cell viability. The results are shown as the mean  SD (n ¼ 6) ***p < 0.001, ###p < 0.001. (E) Hoechst 33258 staining was used to observe changes in nuclear morphology of NB4 cells after GRh2 and C 87 administration (800  ). (F) Quantitative statistical graph of caspase8 cleavage activation levels in NB4 cells. The Ac-IETD-pNA method was used. The results are shown as the mean  SD (n ¼ 3) ***p < 0.001, #p < 0.05. GRh2, 20(S)-ginsenoside Rh2.

Application: WB    Species: Human    Sample: NB4 cells

Fig. 7 GRh2 activated the TNF-α/caspase8 cascade. (A) NB4 cells were incubated with 30 μM, 40 μM, or 50 μM GRh2 for 12 h. Protein expression levels of FasL, Fas, TNF-α, and TNFR1 in NB4 cells were detected via Western blot. A representative picture of three replicates is shown. (B) Quantitative statistical graph of the relative protein expression levels. The results are shown as the mean ± SD (n = 3) ∗p < 0.05, ∗∗p < 0.01. (C) RT-PCR was used to detect the mRNA expression level of TNF-α in NB4 cells after GRh2 administration. The results are shown as the mean ± SD (n = 3) ∗∗p < 0.01, ∗∗∗p < 0.001 versus solvent. After preincubation with 1.5 μM TNF-α inhibitor, C 87, for 2 h, 30 μM, 40 μM, or 50 μM GRh2 was applied for another 12 h. (D) CCK-8 assay measured the NB4 cell viability. The results are shown as the mean ± SD (n = 6) ∗∗∗p < 0.001, ###p < 0.001. (E) Hoechst 33258 staining was used to observe changes in nuclear morphology of NB4 cells after GRh2 and C 87 administration Scale bar=200 μm. (F) Quantitative statistical graph of caspase8 cleavage activation levels in NB4 cells. The Ac-IETD-pNA method was used. The results are shown as the mean ± SD (n = 3) ∗∗∗p < 0.001, #p < 0.05. GRh2, 20(S)-ginsenoside Rh2.
4). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.2]

Application: WB    Species: mouse    Sample: testis

Fig. 7. |SiNPs affected the protein expressions of ATM/p53 and TNF-α/TNFR I-mediated signaling pathways. (A) The protein expressions of ATM, p53, Bcl-2, Bax,TNF-α, TNFR I, Caspase-8, and Caspase-3 were detected in the testis of control group and SiNPs group.
5). Trivalent Chromium Ameliorates Lipid Accumulation and Enhances Glucose Metabolism in the High-NEFA Environment of Bovine Hepatocytes by Regulating PI3K/AKT Pathways. Journal of agricultural and food chemistry, 2024 (PubMed: 39576053) [IF=6.1]
6). Atsttrin regulates osteoblastogenesis and osteoclastogenesis through the TNFR pathway. Communications biology, 2023 (PubMed: 38081906) [IF=5.9]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Fig. 3 Atsttrin inhibited TNF-α-induced osteoclastogenesis via TNFR1. a Western blot analysis to examine the knockdown efficacy of siRNA against TNFR1 in RAW264.7 cells and BMDMs. b BMDMs transfected with scrambled control siRNA (scRNAi) or TNFR1 RNAi were treated with RANKL (100 ng/ml), TNF-α (10 ng/mL), and Atsttrin (500 ng/ml) for 48 h. The protein levels of TRAP and CTSK were measured by Western blotting (n = 3). The bands in the figure are not all derived from the same membrane. c, d Western blot gray value analysis of TNFR1 and TRAP in BMDMs. e, f BMDMs transfected with scrambled control siRNA (scRNAi) or TNFR1 RNAi were cultured with RANKL (100 ng/ml), TNF-α (10 ng/ml), and Atsttrin (500 ng/ml) for 7 days, and TRAP staining was performed. Scale bar, 200 µm. The TRAP-positive multinuclear cells were counted. Each experiment was performed three times independently. g–i RAW264.7 cells transfected with scrambled control siRNA (scRNAi) or TNFR1 RNAi were treated with RANKL (100 ng/ml), TNF-α (10 ng/ml), and Atsttrin (500 ng/ml) for 8 h. The mRNA levels of TRAP, Cathepsin K, and Calcitonin Receptor were measured by real-time PCR (n = 3). Significant differences are indicated as follows: nsP > 0.05, ∗P 
7). Effect of nicotine on placental inflammation and apoptosis in preeclampsia-like model. Life Sciences, 2020 (PubMed: 32835699) [IF=5.2]

Application: IF/ICC    Species: rat    Sample: Placental

Fig. 9. |Nicotine treatment inhibited the expression of TNFR1 (a receptor in the TNF-α-induced extrinsic apoptosis signaling) in placenta in experimental PE rats. (A)Placental tissue sections were stained with anti-TNFR1 by immunofluorescence. Nuclei were visualized with DAPI. White dotted lines show the positive staining.
8). Dingxian pill alleviates hippocampal neuronal apoptosis in epileptic mice through TNF-α/TNFR1 signaling pathway inhibition. Journal of ethnopharmacology, 2024 (PubMed: 39025165) [IF=4.8]
9). Mechanism of Xiaojianzhong decoction in alleviating aspirin-induced gastric mucosal injury revealed by transcriptomics and metabolomics. Journal of ethnopharmacology, 2024 (PubMed: 37453623) [IF=4.8]
10). Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34504840) [IF=4.6]
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