TNF Receptor I Antibody - #AF0282

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产品: TNF Receptor I 抗体
货号: AF0282
描述: Rabbit polyclonal antibody to TNF Receptor I
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 50kDa; 50kD(Calculated).
蛋白号: P19438
RRID: AB_2834164

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(92%), Bovine(%), Horse(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
TNF Receptor I Antibody detects endogenous levels of total TNF Receptor I.
RRID:
AB_2834164
引用格式: Affinity Biosciences Cat# AF0282, RRID:AB_2834164.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD120a; FPF; MGC19588; p55; p55-R; p60; TBP1; TBPI; TNF R; TNF R55; TNF-R1; TNF-RI; TNFAR; TNFR-I; TNFR1; TNFR55; TNFR60; TNFRI; TNFRSF1a; TNR1A_HUMAN; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor superfamily, member 1A; Tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; Tumor necrosis factor-binding protein 1;

抗原和靶标

免疫原:
Uniprot:

P19438(TNR1A_HUMAN) >>Visit HPA database.

基因/基因ID:
TNFRSF1A(7132)
描述:
TNF-R1 Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD.
序列:
MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Pig
92
Rabbit
83
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

翻译修饰:

The soluble form is produced from the membrane form by proteolytic processing.

细胞定位:

Cell membrane>Single-pass type I membrane protein. Golgi apparatus membrane>Single-pass type I membrane protein. Secreted.
Note: A secreted form is produced through proteolytic processing.

Secreted.
Note: Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BABAM2, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts directly with NOL3 (via CARD domain); inhibits TNF-signaling pathway (By similarity). Interacts with SH3RF2, TRADD and RIPK1. SH3RF2 facilitates the recruitment of RIPK1 and TRADD to TNFRSF1A in a TNF-alpha-dependent process.

(Microbial infection) Interacts with mumps virus protein SH; this interaction inhibits downstream NF-kappa-B pathway activation.

(Microbial infection) Interacts with HCV core protein.

(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL138.

(Microbial infection) Interacts with host TNFRSF1A; this interaction leads to the stimulation of both surface expression and shedding of TNFRSF1A.

蛋白家族:

The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.

Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Neutrophil-Mimetic Upconversion Photosynthetic Nanosystem Derived from Microalgae for Targeted Treatment of Thromboembolic Stroke. ACS nano, 2024 (PubMed: 39465976) [IF=15.8]
2). Alpha-(1,6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling. Cell death & disease, 2021 (PubMed: 34857735) [IF=8.1]
3). 20 (S)-Ginsenoside Rh2 induces caspase-dependent PML-RARA degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades. Journal of Ginseng Research, 2021 (PubMed: 33841010) [IF=6.8]

Application: WB    Species: Human    Sample: NB4 cells

Fig. 7. GRh2 activated the TNF-a/caspase8 cascade. (A) NB4 cells were incubated with 30 mM, 40 mM, or 50 mM GRh2 for 12 h. Protein expression levels of FasL, Fas, TNF-a, and TNFR1 in NB4 cells were detected via Western blot. A representative picture of three replicates is shown. (B) Quantitative statistical graph of the relative protein expression levels. The results are shown as the mean  SD (n ¼ 3) *p < 0.05, **p < 0.01. (C) RT-PCR was used to detect the mRNA expression level of TNF-a in NB4 cells after GRh2 administration. The results are shown as the mean  SD (n ¼ 3) **p < 0.01, ***p < 0.001 versus solvent. After preincubation with 1.5 mM TNF-a inhibitor, C 87, for 2 h, 30 mM, 40 mM, or 50 mM GRh2 was applied for another 12 h. (D) CCK-8 assay measured the NB4 cell viability. The results are shown as the mean  SD (n ¼ 6) ***p < 0.001, ###p < 0.001. (E) Hoechst 33258 staining was used to observe changes in nuclear morphology of NB4 cells after GRh2 and C 87 administration (800  ). (F) Quantitative statistical graph of caspase8 cleavage activation levels in NB4 cells. The Ac-IETD-pNA method was used. The results are shown as the mean  SD (n ¼ 3) ***p < 0.001, #p < 0.05. GRh2, 20(S)-ginsenoside Rh2.

Application: WB    Species: Human    Sample: NB4 cells

Fig. 7 GRh2 activated the TNF-α/caspase8 cascade. (A) NB4 cells were incubated with 30 μM, 40 μM, or 50 μM GRh2 for 12 h. Protein expression levels of FasL, Fas, TNF-α, and TNFR1 in NB4 cells were detected via Western blot. A representative picture of three replicates is shown. (B) Quantitative statistical graph of the relative protein expression levels. The results are shown as the mean ± SD (n = 3) ∗p < 0.05, ∗∗p < 0.01. (C) RT-PCR was used to detect the mRNA expression level of TNF-α in NB4 cells after GRh2 administration. The results are shown as the mean ± SD (n = 3) ∗∗p < 0.01, ∗∗∗p < 0.001 versus solvent. After preincubation with 1.5 μM TNF-α inhibitor, C 87, for 2 h, 30 μM, 40 μM, or 50 μM GRh2 was applied for another 12 h. (D) CCK-8 assay measured the NB4 cell viability. The results are shown as the mean ± SD (n = 6) ∗∗∗p < 0.001, ###p < 0.001. (E) Hoechst 33258 staining was used to observe changes in nuclear morphology of NB4 cells after GRh2 and C 87 administration Scale bar=200 μm. (F) Quantitative statistical graph of caspase8 cleavage activation levels in NB4 cells. The Ac-IETD-pNA method was used. The results are shown as the mean ± SD (n = 3) ∗∗∗p < 0.001, #p < 0.05. GRh2, 20(S)-ginsenoside Rh2.
4). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.2]

Application: WB    Species: mouse    Sample: testis

Fig. 7. |SiNPs affected the protein expressions of ATM/p53 and TNF-α/TNFR I-mediated signaling pathways. (A) The protein expressions of ATM, p53, Bcl-2, Bax,TNF-α, TNFR I, Caspase-8, and Caspase-3 were detected in the testis of control group and SiNPs group.
5). Trivalent Chromium Ameliorates Lipid Accumulation and Enhances Glucose Metabolism in the High-NEFA Environment of Bovine Hepatocytes by Regulating PI3K/AKT Pathways. Journal of agricultural and food chemistry, 2024 (PubMed: 39576053) [IF=6.1]
6). Atsttrin regulates osteoblastogenesis and osteoclastogenesis through the TNFR pathway. Communications biology, 2023 (PubMed: 38081906) [IF=5.9]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Fig. 3 Atsttrin inhibited TNF-α-induced osteoclastogenesis via TNFR1. a Western blot analysis to examine the knockdown efficacy of siRNA against TNFR1 in RAW264.7 cells and BMDMs. b BMDMs transfected with scrambled control siRNA (scRNAi) or TNFR1 RNAi were treated with RANKL (100 ng/ml), TNF-α (10 ng/mL), and Atsttrin (500 ng/ml) for 48 h. The protein levels of TRAP and CTSK were measured by Western blotting (n = 3). The bands in the figure are not all derived from the same membrane. c, d Western blot gray value analysis of TNFR1 and TRAP in BMDMs. e, f BMDMs transfected with scrambled control siRNA (scRNAi) or TNFR1 RNAi were cultured with RANKL (100 ng/ml), TNF-α (10 ng/ml), and Atsttrin (500 ng/ml) for 7 days, and TRAP staining was performed. Scale bar, 200 µm. The TRAP-positive multinuclear cells were counted. Each experiment was performed three times independently. g–i RAW264.7 cells transfected with scrambled control siRNA (scRNAi) or TNFR1 RNAi were treated with RANKL (100 ng/ml), TNF-α (10 ng/ml), and Atsttrin (500 ng/ml) for 8 h. The mRNA levels of TRAP, Cathepsin K, and Calcitonin Receptor were measured by real-time PCR (n = 3). Significant differences are indicated as follows: nsP > 0.05, ∗P 
7). Effect of nicotine on placental inflammation and apoptosis in preeclampsia-like model. Life Sciences, 2020 (PubMed: 32835699) [IF=5.2]

Application: IF/ICC    Species: rat    Sample: Placental

Fig. 9. |Nicotine treatment inhibited the expression of TNFR1 (a receptor in the TNF-α-induced extrinsic apoptosis signaling) in placenta in experimental PE rats. (A)Placental tissue sections were stained with anti-TNFR1 by immunofluorescence. Nuclei were visualized with DAPI. White dotted lines show the positive staining.
8). Dingxian pill alleviates hippocampal neuronal apoptosis in epileptic mice through TNF-α/TNFR1 signaling pathway inhibition. Journal of ethnopharmacology, 2024 (PubMed: 39025165) [IF=4.8]
9). Mechanism of Xiaojianzhong decoction in alleviating aspirin-induced gastric mucosal injury revealed by transcriptomics and metabolomics. Journal of ethnopharmacology, 2024 (PubMed: 37453623) [IF=4.8]
10). Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34504840) [IF=4.6]
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