C-RAF Antibody - #AF0837
产品: | C-RAF 抗体 |
货号: | AF0837 |
描述: | Rabbit polyclonal antibody to C-RAF |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat, Monkey |
预测: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 73kDa; 73kD(Calculated). |
蛋白号: | P04049 |
RRID: | AB_2834122 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0837, RRID:AB_2834122.
展开/折叠
c Raf; C-raf; C-Raf proto-oncogene, serine/threonine kinase; CMD1NN; Craf 1 transforming gene; cRaf; Craf1 transforming gene; EC 2.7.11.1; kinase Raf1; Murine sarcoma 3611 oncogene 1; NS5; Oncogene MIL; Oncogene RAF1; OTTHUMP00000160218; OTTHUMP00000207813; OTTHUMP00000209389; Protein kinase raf 1; Proto-oncogene c-RAF; Raf 1; Raf 1 proto oncogene serine/threonine kinase; RAF; Raf proto oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF-1; RAF1; RAF1_HUMAN; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1; TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV; v raf 1 murine leukemia viral oncogene homolog 1; v-raf murine sarcoma viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine leukemia viral oncogene homolog 1;
抗原和靶标
- P04049 RAF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P04049 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S12 | Phosphorylation | Uniprot | |
K18 | Methylation | Uniprot | |
S29 | Phosphorylation | P28482 (MAPK1) | Uniprot |
T31 | Phosphorylation | Uniprot | |
Y38 | Phosphorylation | Uniprot | |
S43 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) | Uniprot |
T49 | Phosphorylation | Uniprot | |
K53 | Ubiquitination | Uniprot | |
K65 | Ubiquitination | Uniprot | |
S211 | Phosphorylation | Uniprot | |
T213 | Phosphorylation | Uniprot | |
S220 | Phosphorylation | Uniprot | |
R223 | Methylation | Uniprot | |
R231 | Methylation | Uniprot | |
Y232 | Phosphorylation | Uniprot | |
S233 | Phosphorylation | P17612 (PRKACA) , P17252 (PRKCA) | Uniprot |
T234 | Phosphorylation | Uniprot | |
T239 | Phosphorylation | Uniprot | |
T242 | Phosphorylation | Uniprot | |
S244 | Phosphorylation | Uniprot | |
S247 | Phosphorylation | Uniprot | |
S250 | Phosphorylation | Uniprot | |
S252 | Phosphorylation | Uniprot | |
S257 | Phosphorylation | Uniprot | |
T258 | Phosphorylation | Uniprot | |
S259 | Phosphorylation | O95835 (LATS1) , P17612 (PRKACA) , P31749 (AKT1) , Q13131 (PRKAA1) , P04049 (RAF1) | Uniprot |
T260 | Phosphorylation | Uniprot | |
S267 | Phosphorylation | Uniprot | |
T268 | Phosphorylation | P04049 (RAF1) | Uniprot |
T269 | Phosphorylation | Q8IVT5 (KSR1) | Uniprot |
S283 | Phosphorylation | Uniprot | |
S285 | Phosphorylation | Uniprot | |
S287 | Phosphorylation | Uniprot | |
S289 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
S291 | Phosphorylation | Uniprot | |
S294 | Phosphorylation | Uniprot | |
S295 | Phosphorylation | Uniprot | |
S296 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
S301 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
T303 | Phosphorylation | Uniprot | |
S306 | Phosphorylation | Uniprot | |
T310 | Phosphorylation | Uniprot | |
T324 | Phosphorylation | Uniprot | |
S338 | Phosphorylation | Q02750 (MAP2K1) , P04049 (RAF1) , O75914 (PAK3) , P53350 (PLK1) , Q13153 (PAK1) , Q16512 (PKN1) | Uniprot |
S339 | Phosphorylation | P53350 (PLK1) , O75914 (PAK3) , Q16512 (PKN1) , Q13153 (PAK1) | Uniprot |
Y340 | Phosphorylation | P12931 (SRC) | Uniprot |
Y341 | Phosphorylation | P12931 (SRC) | Uniprot |
T353 | Phosphorylation | Uniprot | |
S357 | Phosphorylation | Uniprot | |
S359 | Phosphorylation | Uniprot | |
T362 | Phosphorylation | Uniprot | |
K365 | Ubiquitination | Uniprot | |
K367 | Ubiquitination | Uniprot | |
K378 | Ubiquitination | Uniprot | |
Y458 | Phosphorylation | Uniprot | |
K462 | Ubiquitination | Uniprot | |
K470 | Ubiquitination | Uniprot | |
S471 | Phosphorylation | Uniprot | |
T481 | Phosphorylation | Uniprot | |
T491 | Phosphorylation | Uniprot | |
K493 | Ubiquitination | Uniprot | |
S494 | Phosphorylation | Uniprot | |
S497 | Phosphorylation | P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA) | Uniprot |
S499 | Phosphorylation | P17252 (PRKCA) | Uniprot |
K575 | Ubiquitination | Uniprot | |
S612 | Phosphorylation | Uniprot | |
S619 | Phosphorylation | P05129 (PRKCG) , P05771 (PRKCB) , P17252 (PRKCA) | Uniprot |
S621 | Phosphorylation | P17612 (PRKACA) , P04049 (RAF1) , Q13131 (PRKAA1) | Uniprot |
S624 | Phosphorylation | Uniprot | |
T638 | Phosphorylation | Uniprot | |
T640 | Phosphorylation | Uniprot | |
S642 | Phosphorylation | P28482 (MAPK1) | Uniprot |
翻译修饰 - P04049 作为激酶
Substrate | Site | Source |
---|---|---|
O14974 (PPP1R12A) | T696 | Uniprot |
O43306 (ADCY6) | S732 | Uniprot |
O43306 (ADCY6) | S734 | Uniprot |
O43306 (ADCY6) | S738 | Uniprot |
O43306 (ADCY6) | S742 | Uniprot |
P01106 (MYC) | T8 | Uniprot |
P04049 (RAF1) | S259 | Uniprot |
P04049-1 (RAF1) | T268 | Uniprot |
P04049 (RAF1) | S338 | Uniprot |
P04049-1 (RAF1) | S621 | Uniprot |
P28482-1 (MAPK1) | S29 | Uniprot |
P45379 (TNNT2) | T213 | Uniprot |
Q02750 (MAP2K1) | S218 | Uniprot |
Q02750-1 (MAP2K1) | S222 | Uniprot |
Q02750-1 (MAP2K1) | T286 | Uniprot |
Q02750-1 (MAP2K1) | T292 | Uniprot |
Q02750-1 (MAP2K1) | T386 | Uniprot |
Q05639 (EEF1A2) | S21 | Uniprot |
Q13118 (KLF10) | T93 | Uniprot |
Q92934 (BAD) | S75 | Uniprot |
Q92934 (BAD) | S99 | Uniprot |
Q92934 (BAD) | S118 | Uniprot |
研究背景
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. Phosphorylation at Ser-338 by PAK1 and PAK5 and Ser-339 by PAK1 is required for its mitochondrial localization. Phosphorylation at Ser-621 in response to growth factor treatment stabilizes the protein, possibly by preventing proteasomal degradation. Phosphorylation at Ser-289, Ser-296, Ser-301, Ser-338 and Ser-621 are somehow linked to the methylation potential of cells. Treatment of cells with HGF in the presence of the methylation inhibitor 5'-methylthioadenosine (MTA) results in increased phosphorylation at Ser-338 and Ser-621 and decreased phosphorylation at Ser-296, Ser-301 and Ser-338. Dephosphorylation at Ser-338 by PPP5C results in an activity decrease.
Methylated at Arg-563 in response to EGF treatment. This modification leads to destabilization of the protein, possibly through proteasomal degradation.
Cytoplasm. Cell membrane. Mitochondrion. Nucleus.
Note: Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
In skeletal muscle, isoform 1 is more abundant than isoform 2.
Monomer. Homodimer. Heterodimerizes with BRAF and this heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer. Forms a multiprotein complex with Ras (M-Ras/MRAS), SHOC2 and protein phosphatase 1 (PPP1CA, PPP1CB and PPP1CC). Interacts with LZTR1. Interacts with Ras proteins; the interaction is antagonized by RIN1. Weakly interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with BRAF, a ternary complex inhibited by GNAI1 (By similarity). Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Interacts with STK3/MST2; the interaction inhibits its pro-apoptotic activity. Interacts (when phosphorylated at Ser-259) with YWHAZ (unphosphorylated at 'Thr-232'). Interacts with MAP2K1/MEK1 and MAP2K2/MEK2 (By similarity). Interacts with MAP3K5/ASF1 (via N-terminus) and this interaction inhibits the proapoptotic function of MAP3K5/ASK1. Interacts with PAK1 (via kinase domain). The phosphorylated form interacts with PIN1 (By similarity). The Ser-338 and Ser-339 phosphorylated form (by PAK1) interacts with BCL2. Interacts with PEBP1/RKIP and this interaction is enhanced if RAF1 is phosphorylated on residues Ser-338, Ser-339, Tyr-340 and Tyr-341. Interacts with ADCY2, ADCY5, ADCY6, DGKH, RCAN1/DSCR1, PPP1R12A, PKB/AKT1, PPP2CA, PPP2R1B, SPRY2, SPRY4, CNKSR1/CNK1, KSR2 and PHB/prohibitin. Interacts with ROCK2 (By similarity). In its active form, interacts with PRMT5. Interacts with FAM83B; displaces 14-3-3 proteins from RAF1 and activates RAF1. Interacts with PDE8A; the interaction promotes RAF1 activity. Interacts with MFHAS1. Interacts with GLS.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
文献引用
Application: WB Species: mouse Sample: lung
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