Phospho-RIPK2 (Ser176) Antibody - #AF0049

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产品: 磷酸化 RIPK2 (Ser176) 抗体
货号: AF0049
描述: Rabbit polyclonal antibody to Phospho-RIPK2 (Ser176)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: O43353
RRID: AB_2834094

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 50ul RMB¥ 1300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-RIPK2 (Ser176) Antibody detects endogenous levels of RIPK2 only when phosphorylated at Sersine 176.
RRID:
AB_2834094
引用格式: Affinity Biosciences Cat# AF0049, RRID:AB_2834094.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CARD 3; CARD carrying kinase; CARD containing ICE associated kinase; CARD containing IL 1 beta ICE kinase; CARD containing IL1 beta ICE kinase; CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase; CARD containing interleukin 1 beta converting enzyme associated kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARD3; CARDIAK; CCK; CLARP kinase; GIG 30; GIG30; Growth inhibiting gene 30; Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase; Receptor interacting protein 2; Receptor interacting serine threonine kinase 2; Receptor interacting serine/threonine protein kinase 2; Receptor-interacting protein 2; Receptor-interacting serine/threonine-protein kinase 2; RICK; RIP 2; RIP like interacting CLARP kinase; RIP-2; RIP-like-interacting CLARP kinase; RIPK 2; Ripk2; RIPK2_HUMAN; TNFRSF; Tyrosine-protein kinase RIPK2; UNQ277/PRO314/PRO34092;

抗原和靶标

免疫原:

A synthesized peptide derived from human RIPK2 around the phosphorylation site of Ser176.

基因/基因ID:
RIPK2(8767)
描述:
RIPK2 a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB. Note: This description may include information from UniProtKB.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

文献引用

1). Activation of NOD1 on tumor-associated macrophages augments CD8+ T cell-mediated antitumor immunity in hepatocellular carcinoma. Science advances, 2024 (PubMed: 39356756) [IF=11.7]
2). Elevated muramyl dipeptide by sialic acid-facilitated postantibiotic pathobiont expansion contributes to gut dysbiosis-induced mastitis in mice. Journal of advanced research, 2024 (PubMed: 39374734) [IF=11.4]
3). Sodium valproate attenuates the iE-DAP induced inflammatory response by inhibiting the NOD1-NF-κB pathway and histone modifications in bovine mammary epithelial cells. International Immunopharmacology, 2020 (PubMed: 32182568) [IF=4.8]

Application: WB    Species: bovine    Sample: BMECs

Fig. 4. |Protein expression of NOD1-mediated NF-κB signaling pathway genes and the expression and location of NOD1 and NF-κB p65 protein in BMECs. The cells were treated with iE‐DAP (10 μg/mL) or VPA (0.5 mmol/L) or pretreatment with VPA (0.5 mmol/L) followed by 10 μg/mL of iE‐DAP alone. Western blot analysis showing the NOD1, RIPK2 (A, B), p65, p-p65, IκB and p-IκB (C, D) levels. The protein expression was normalized by the respective abundance of β-actin and βtubulin.
4). C/EBPβ Promotes LPS-Induced IL-1β Transcription and Secretion in Alveolar Macrophages via NOD2 Signaling. Journal of inflammation research, 2022 (PubMed: 36120185) [IF=4.5]

Application: WB    Species: Rat    Sample: NR8383 cells

Figure 4 C/EBPβ targets the NOD2/RIPK2/NF-κB pathway to regulate IL-1β transcription and secretion. (A–C) The protein and mRNA levels of NOD1 and NOD2 were detected using WB and RT-qPCR in LPS-treated NR8383 cells transfected with sh-NC or sh-C/EBPβ. (D) The agonistic effect of MDP on NOD2 receptor was detected by RT-qPCR. (E and F) The level of IL-1β was detected via RT-qPCR and ELISA in response to sh-C/EBPβ or sh-C/EBPβ + MDP in LPS-induced NR8383 cells. (G and H) WB and its histogram were used to assess the expression levels of NOD2, RIPK2, p-RIPK2, NF-κB p65, NF-κB p-p65, Pro-IL-1β and IL-1β p17 in LPS-intoxicated NR8383 cells transfected with different plasmids. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the CON group. #p < 0.05, ##p < 0.01, and ###p < 0.001 compared with the LPS/LPS + sh-NC group. ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 compared with the LPS + sh-C/EBPβ group.
5). Shikonin attenuates cerebral ischemia/reperfusion injury via inhibiting NOD2/RIP2/NF-κB-mediated microglia polarization and neuroinflammation. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 2024 (PubMed: 38527567) [IF=2.0]

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