IRAK4 Antibody - #DF2667

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产品: IRAK4 抗体
货号: DF2667
描述: Rabbit polyclonal antibody to IRAK4
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q9NWZ3
RRID: AB_2839873

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IRAK4 Antibody detects endogenous levels of total IRAK4.
RRID:
AB_2839873
引用格式: Affinity Biosciences Cat# DF2667, RRID:AB_2839873.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IL-1 receptor-associated kinase 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64; NY REN 64 antigen; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64;

抗原和靶标

免疫原:

A synthesized peptide derived from human IRAK4, corresponding to a region within the internal amino acids.

基因/基因ID:
IRAK4(51135)
描述:
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.;

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

文献引用

1). The matrix protein of Newcastle disease virus inhibits inflammatory response through IRAK4/TRAF6/TAK1/NF-κB signaling pathway. International Journal of Biological Macromolecules, 2022 (PubMed: 35872314) [IF=7.7]
2). The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells. Life sciences, 2024 (PubMed: 38103725) [IF=5.2]
3). Artemisinin attenuates perinatal inflammation and consequent oxidative stress in oligodendrocyte precursor cells by inhibiting IRAK-4 and IRAK-1. International immunopharmacology, 2024 (PubMed: 39293313) [IF=4.8]

Application: WB    Species: Rat    Sample: OPCs

Fig. 4. LPS-induced activation of the IRAK-4/IRAK-1/NF-κB signalling pathway in OPCs was inhibited by ART. a, b, c, d Western blotting was used to quantify the protein levels of p-IRAK-4, IRAK-4, p-IRAK-4/IRAK-4, p-IRAK-1, IRAK-1, p-IRAK-1/IRAK-1, phospho-NF-kB p65, NF-κB p65, and phospho-NF-kB p65/NF-κB p65 in 4 groups (the Ctrl, ART, LPS, and LPS + ART groups). The data are expressed as the means ± SEMs (n = 6/group) (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).
4). Patchouli oil ameliorates 5-fluorouracil-induced intestinal mucositis in rats via protecting intestinal barrier and regulating water transport. JOURNAL OF ETHNOPHARMACOLOGY, 2020 (PubMed: 31883475) [IF=4.8]

Application: WB    Species: rat    Sample: intestinal

Fig. 8.| Effect of P.oil on the expression of TLRs-MyD88 pathway-related proteins (n = 3–5). A: representative western blotting band; B: The expressions of MyD88(a),IRAK-4(b), FADD (c), TAK-1(d), p-p38/p38 (e) and p-p65/p65 (f).
5). Disulfiram attenuates cell and tissue damage and blood‒brain barrier dysfunction after intracranial haemorrhage by inhibiting the classical pyroptosis pathway. Scientific reports, 2024 (PubMed: 39300102) [IF=3.8]
6). Pharmacological inhibition of IRAK1 attenuates colitis‐induced tumorigenesis in mice by inhibiting the inflammatory response and epithelial–mesenchymal transition. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2021 (PubMed: 34273909) [IF=3.2]

Application: WB    Species: Human    Sample: HCT‐116 cells

FIGURE 4 IRAK1/4 inhibitor treatment inhibits epithelial–mesenchymal transition (EMT) in mice by reducing TLR/IL‐1R‐mediated NF‐κB activation. (A) IRAK1/4 inhibitor treatment decreased the expression of phosphorylated IRAK4, IRAK1, and P65. (B) IRAK1/4 inhibitor treatment inhibits EMT induced by exposure to azoxymethane/dextran sodium sulfate (AOM/DSS). Values are expressed as mean±SD. # Significantly different compared with control, p<0.05; *significantly different compared with model, p <0.05
7). IRAK4 inhibition: an effective strategy for immunomodulating peri-implant osseointegration via reciprocally-shifted polarization in the monocyte-macrophage lineage cells. BMC oral health, 2023 (PubMed: 37158847) [IF=2.6]

Application: WB    Species: Rat    Sample: BMMs

Fig. 1 The polarization and differentiation of the bone monocyte/macrophage lineage cells on SLA surface under inflammatory conditions. A BMMs were cultured on the SLA surfaces in the presence of M-CSF (30 ng/ml) with or without IL-1β and IRAK4 inhibitor (IRAK4i) for 1, 3, 7 days, and then the cell viability was evaluated by AlamarBlue assay. B Representative images of immunofluorescent staining of BMMs cultured on SLA surfaces for 24 h. DAPI, blue; CCR7 (M1 marker), red; CD163 (M2 marker), green. C Semiquantitative analysis of the mean immunofluorescence intensity of BMMs. D Western blotting of IRAK4, M1 Mø (iNOS, CCR7), M2 Mø (CD206, CD163) of BMMs after cultured on the SLA surfaces with the addition of Il-1β or IRAK4i. E Quantitative analysis of the relative level of proteins. F The transcriptional levels of inflammation-related and wound healing-related genes of BMMs were evaluated via RT-qPCR. *p 

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