VEGFC Antibody - #DF7011

Figure 7. NET degradation improves mLV damage. C57BL/6 mice were i.c.v. injected with or without DNase I after IVH. Twenty-four h post-IVH, the meninges were collected for observation. (A) Representative WB images showing the expression of CitH3, VEGFC, LYVE-1, PROX1, FOXC2, and VE-cadherin in the meninges in the Sham, IVH, and IVH+DNase I groups 24 h after IVH. GAPDH was used as an internal reference protein. (B-G) Quantification of CitH3, VEGFC, LYVE-1, PROX1, FOXC2, and VE-cadherin expression (n = 6, one-way ANOVA). (H, I) Relative mRNA levels of FOXC2 and VEGFC in the meninges in the various experimental groups (n = 6). (J, K) The concentrations of VEGFC and FOXC2 in the meninges were quantified by ELISA (n = 6, one-way ANOVA). (L) NO concentrations in the meninges were quantifid in the different groups 24 h after IVH (n = 6, one-way ANOVA). (M) A representative image showing the IF staining of the meninges with anti-LYVE-1 antibodies (red) in the COS. (N) The extent of anti-LYVE-1 antibody staining in the meninges of the COS was quantified and is presented as a percentage of the total area (n = 6, one-way ANOVA). (O) Images showing TUNEL and LYVE-1 staining in the TS of Sham-, IVH-, or DNase I-treated mice were selected as representative examples 24 h after IVH. (P) The number of TUNEL-positive LECs surrounding the TS in the meninges of mice (n = 6, one-way ANOVA). *P < 0.05, **p < 0.01. Data are means ± SEMs.

FIGURE 3|Effect of TRAF6 expression on NF-κB, pNF-κB, and VEGF-C expression in SW480 and HCT116 cells. (A, B) Western blotting analysis of NF-κB, pNF-κB, and VEGF-C expression in SW480 and HCT116 cells transfected with pCDH and pCDH-TRAF6, respectively.GAPDH served as a loading control.

Figure 5.Identification of growth-factor secretion in human γδ T+ Cells. (A) qRT-PCR results from eight selected genes using RNA-sequencing. (B) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from early decidua (n = 5). (C) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from term decidua (n = 5). (D) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from peripheral blood mononuclear cells (PBMCs) (n = 5). (E) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from recurrent spontaneous abortion (RSA) patients (n = 5; scale bar, 50 μm). (F) qRT-PCR results regarding growth factors in sorted γδ T cells from RSA patients (n = 5). P-value was calculated by unpaired Student’s t-test.

Figure 4. | The effect of exercise on lung cancer tissue angiogenesis and lymphangiogenesis.E, F. The effect of exercise on the expression of VEGFA in lung cancer tissues (n=6). E, G. The effect of exercise on the expression of VEGFC in lung cancer tissues (n=6). Note: &, && respectively represent a significant difference compared with the Saline group (P<0.05),(P<0.01).

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.