TLR2 Antibody - #DF7002

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产品: TLR2 抗体
货号: DF7002
描述: Rabbit polyclonal antibody to TLR2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Dog
蛋白号: O60603
RRID: AB_2838958

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TLR2 Antibody detects endogenous levels of total TLR2.
RRID:
AB_2838958
引用格式: Affinity Biosciences Cat# DF7002, RRID:AB_2838958.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD282; CD282 antigen; TIL 4; TIL4; TLR 2; TLR2; TLR2_HUMAN; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4; Toll/interleukin-1 receptor-like protein 4;

抗原和靶标

免疫原:

A synthesized peptide derived from human TLR2, corresponding to a region within the internal amino acids.

基因/基因ID:
TLR2(7097)
描述:
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

文献引用

1). A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. BIOMATERIALS, 2020 (PubMed: 32554130) [IF=12.8]

Application: WB    Species: mouse    Sample: Macrophages

Figure 6. a) CPHs treatment activated the expression of HO-1 in LPS-induced activated macrophages (n = 5), the expression levels of HO-1 in LPS-induced activated macrophages vs that of the free Dex-p, CPs and CPHs treated activated macrophages, b) CPHs treatment downregulated the expression of HO-1, p38, p50, p65 and TLR-2, c-f) Relative p38, p50, p65 and TLR-2 expression level. Free Dex-p and CPs served as control. (n = 3). The relative expression levels of p38, p50, p65 and TLR-2 in LPS-induced activated macrophages vs that of the free Dex-p, CPs and CPHs treated activated macrophages, * p < 0.05, ** p < 0.01, *** p < 0.001.
2). A novel UV-curable extravascular stent to prevent restenosis of venous grafts. Composites Part B: Engineering, 2021 [IF=12.7]

Application: WB    Species: Rat    Sample:

Fig. 7. A: Western blot analysis of TLR2/MyD88/NF-κB pathway proteins in venous graft vessels. B: Contents of TLR2/MyD88/NF-κB pathway proteins in venous graft vessels. Data are presented as the mean ± SD; *: compared with control group, p < 0.05. #: compared with sham group, p < 0.05.
3). Mechanisms underlying the effects of the conditional knockdown of hepatic PCSK9 in attenuating lipopolysaccharide-induced acute liver inflammation. International journal of biological macromolecules, 2025 (PubMed: 39716700) [IF=7.7]
4). Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 32004938) [IF=6.9]

Application: WB    Species: rat    Sample:

Fig. 3.| Effect of PA on inflammtory cytokines (n = 8) and TLR2/MyD88/NF-κB pathway proteins (n = 3). (a–e) Levels of TNF-α, IL-1β, IL-6, IL-10, and MPO; (f–h)Expressions of TLR2 and MyD88 proteins
5). Isolation and identification of immunomodulatory peptides from the protein hydrolysate of tuna trimmings (Thunnas albacares). LWT, 2022 [IF=6.0]
6). Inhibition of murine herpesvirus-68 replication by IFN-gamma in macrophages is counteracted by the induction of SOCS1 expression. PLOS Pathogens, 2023 (PubMed: 30075008) [IF=5.5]

Application: WB    Species: mouse    Sample: macrophages

Fig 6. |TLR3 mediates the MHV-68-induced SOCS1 production. (A) BMMs were transfected with siRNAs against Tlr2, Tlr3, Tlr4, Tlr7, Tlr9 (si-TLR) or an irrelevant target (si-Control), respectively. Forty-eight hours post transfection, cells were infected with MHV-68 at MOI = 10. At 8 hpi, RNA and protein were then extracted. RT-qPCR was performed to determine SOCS1 mRNA levels and western blotting was performed to determine the protein expression levels of SOCS1 and the individual TLRs, respectively. The SOCS1 mRNA levels are expressed as values relative to the MHV-68 infected, si-Control transfected cells.
7). Novel chimeric TLR2/NOD2 agonist CL429 exhibited significant radioprotective effects in mice. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33609010) [IF=5.3]

Application: WB    Species: Mice    Sample:

FIGURE 5 The mechanism for the radioprotective effect of CL429. (A) C57BL/6 mice and TLR2 KO mice were treated with CL429 before TBI, and then, the survival was monitored (WT n = 11, TLR2 KO n = 7). (B) The representative pathological images of haematopoietic system and gastrointestinal tract at 3.5 d after radiation. (C) The protein expression of TLR2 and NOD2 after siRNA knock‐down. Cell viability was determined using CCK‐8 at 24 h after radiation. *P < .05 vs IR groups. (D) The effects of CL429 on TLR2, NOD2, MyD88 and p‐IKK at 0 h, 2 h, 4 h, 8 h, 12 h and 24 h were measured by Western blot assay. (E) IL‐6, IL‐11, IL‐12 and TNF‐α were detected by ELISA 24 h after radiation. *P < .05, **P < .01 vs IR + PBS groups
8). Isorhamnetin Ameliorates Aspergillus fumigatus Keratitis by Reducing Fungal Load, Inhibiting Pattern-Recognition Receptors and Inflammatory Cytokines. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2021 (PubMed: 33783487) [IF=5.0]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Figure 6. Effects of isorhamnetin on the inflammatory response in A. fumigatus infected mouse cornea. qRT-PCR results for TLR-2 (A), TLR-4 (B), Dectin-1 (C) TNF-α (J) and IL-1β (K) at 1,3, and 5 days p. i. Western blot results and grayscale analysis of TLR-2 (D, G), TLR-4 (E, H), Dectin-1 (F, I) at 3 days p. i. ELISA results of TNF-α (L) and IL-1β (M) at 3 and 5 days p. i. (n = 6 mice/group). All data were mean ± SEM and analyzed by an unpaired, two-tailed Student's t-test.
9). Taurine reduction of injury from neutrophil infiltration ameliorates Streptococcus uberis-induced mastitis. International immunopharmacology, 2023 (PubMed: 37857121) [IF=4.8]
10). 25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway. International Journal of Oncology, 2019 (PubMed: 30664194) [IF=4.5]
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