IRF3 Antibody - #DF6895

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产品: IRF3 抗体
货号: DF6895
描述: Rabbit polyclonal antibody to IRF3
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: Q14653
RRID: AB_2838854

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IRF3 Antibody detects endogenous levels of total IRF3.
RRID:
AB_2838854
引用格式: Affinity Biosciences Cat# DF6895, RRID:AB_2838854.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IIAE7; Interferon regulatory factor 3; IRF 3; IRF-3; IRF3; IRF3_HUMAN; MGC94729;

抗原和靶标

免疫原:

A synthesized peptide derived from human IRF3, corresponding to a region within C-terminal amino acids.

基因/基因ID:
IRF3(3661)
描述:
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3 (8).

研究领域

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39703158) [IF=15.1]
2). Novel intravenous formulation for radiosensitization in osteosarcoma treatment. Materials today. Bio, 2025 (PubMed: 40206141) [IF=8.7]
3). Up-regulated lncRNA CYLD as a ceRNA of miR-2383 facilitates bovine viral diarrhea virus replication by promoting CYLD expression to counteract RIG-I-mediated type-I IFN production. International journal of biological macromolecules, 2023 (PubMed: 37839600) [IF=7.7]
4). Commensal cow Roseburia reduces gut-dysbiosis-induced mastitis through inhibiting bacterial translocation by producing butyrate in mice. Cell Reports, 2022 (PubMed: 36417859) [IF=7.5]
5). High-Intensity Interval Training Ameliorates High-Fat Diet-Induced Metabolic Disorders via the Cyclic GMP-AMP Synthase-Stimulator of Interferon Gene Signaling Pathway. International journal of molecular sciences, 2023 (PubMed: 37762143) [IF=5.6]

Application: WB    Species: Mouse    Sample:

Figure 4. Effects of HIIT and MICT on the cGAS-STING signaling pathway in skeletal muscle of HFD mice. (A–D) Expression of cGAS-STING signaling pathway-related proteins (n = 3 for each group). (E,F) Serum IL-6 and IFN-β levels (n = 7 for HFD, n = 7 for MH, n = 8 for HH). All data are presented as mean ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Group HFD.
6). Honokiol ameliorates silica-induced lung fibrosis by inhibiting macrophage pyroptosis via modulating cGAS/STING signaling. International immunopharmacology, 2024 (PubMed: 39681061) [IF=5.6]
7). Zinc Finger CCCH-Type Antiviral Protein 1 Restricts the Viral Replication by Positively Regulating Type I Interferon Response. Frontiers in Microbiology, 2020 (PubMed: 32922375) [IF=5.2]

Application: WB    Species:    Sample: ZC3HAV1 knockdown cells

FIGURE S1 | (A) The positive cells were observed under fluorescent microscope to detect the expression of GFPs. (B) RT-PCR was performed to detect the expression of ZC3HAV1, IFN-β, MxA, TNF, and IL-6 mRNAs in A549 cells expressing sh-ZC3HAV1 or sh-Luc in response to WSN infection. (D) Western blotting was performed to detect the protein expression of IRF3 and p-IRF3 in ZC3HAV1 knockdown cells after the viral infection.
8). STING Activation in Macrophages and Microglia Drives Poststroke Inflammation: Implications for Neuroinflammatory Mechanisms and Therapeutic Interventions. CNS neuroscience & therapeutics, 2024 (PubMed: 39698742) [IF=4.8]

Application: WB    Species: Mouse    Sample:

FIGURE 5 Prolonged phagocytosis facilitates STING (stimulator of interferon genes) activation and phenotypic shift in microglia and macrophages. A mixture of brain-derived danger-associated molecular patterns (DAMPs) was extracted from naïve mouse brains and administered to primary macrophage and microglia for short-term (24 h) and prolonged (48 h) phagocytosis. Following treatment, we assessed the inflammatory properties and STING activation in microglia and macrophages. Representative plots and the percentage of TNF-α+/IL-10+ macrophages (A) and microglia (B) in control, short-term, and prolonged phagocytosis groups. Experiments were conducted four times. *p 
9). Electroacupuncture alleviates the symptom of depression in mice by regulating the cGAS-STING-NLRP3 signaling. Aging, 2024 (PubMed: 38643466) [IF=3.9]
10). Corilagin Interferes With Toll-Like Receptor 3-Mediated Immune Response in Herpes Simplex Encephalitis. Frontiers in Molecular Neuroscience, 2019 (PubMed: 31080403) [IF=3.5]

Application: WB    Species: mouse    Sample: BV-2 cells

Figure 4.| Lentiviral vector-mediated overexpression of TLR3 in BV-2 cells. The lentivirus (10 MOI) was incubated with the BV-2 cells for 72 h. (A) The expression of GFP was observed with a fluorescence microscope after lentivirus was introduced into the BV-2 cells. (B) The mRNA levels of TLR3 and its downstream molecules were measured by RT-PCR. (C) The protein levels were detected by western blotting. Data are shown as the mean ± SD of three independent experiments of triplicate samples.**P < 0.01 for lentivirus-up vs. normal; *P < 0.05 for lentivirus-up vs. normal (by Student’s t-test).
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