(1)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6602, RRID:AB_2838564.
展开/折叠
38 kDa Mov34 homolog; COP9 (constitutive photomorphogenic) homolog subunit 5; COP9 constitutive photomorphogenic homolog subunit 5; COP9 signalosome complex subunit 5; COP9 signalosome subunit 5; Cop9 subunit 5; COPS 5; cops5; CSN 5; CSN5; CSN5_HUMAN; JAB 1; Jun activation domain binding protein 1; Jun activation domain binding protein; Jun activation domain-binding protein 1; MGC3149; MOV 34; MOV34; MOV34 family, 38-KD member; SGN 5; SGN5; Signalosome subunit 5;
抗原和靶标
- Q92905 CSN5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92905 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| R282 | Methylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| R294 | Methylation | Uniprot | |
| K295 | Methylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K326 | Acetylation | Uniprot | |
| K326 | Ubiquitination | Uniprot |
研究背景
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.
Cytoplasm>Cytosol. Nucleus. Cytoplasm>Perinuclear region. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.
Note: Nuclear localization is diminished in the presence of IFIT3.
Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B), COPS8 and COPS9 isoform 1. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B) and COPS9 isoform 1. Interacts with COPS9 isoform 2. The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, HIF1A, CDKN1B, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ID1, ID3, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Interacts with IFIT3. Interacts with BRSK2. Interacts with ZDHHC16. Interacts with MINDY3. Interacts with FANK1; regulates the phosphorylation of JUN and the transcriptional activity of AP-1. Interacts with NUPR1; this interaction allows COPS5-dependent CDKN1B nuclear to cytoplasm translocation.
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex (By similarity).
Belongs to the peptidase M67A family. CSN5 subfamily.
文献引用
Application: WB Species: Human Sample: SW620 cells
Application: IHC Species: Human Sample: SW620 cells
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