TLR8 Antibody - #DF6426

FIGURE 7 Validation of the hub genes. (A) The network of hub genes of the brown module is shown. Nodes represent the genes and node size indicate the betweenness score. (B) Fluorescence graph of TLR8 knockdown in cell proliferation experiment at 100× magnification. (C) Line graph of cell proliferation and cell proliferation fold change after TLR8 knockdown. (D) Kaplan–Meier curve showed that overall survival (OS) was worse when TLR8 was highly expressed (log-rank test, p = 0.029). (E) Immunohistochemistry images of the three immunophenotypes. scale bars, 50 μm. (F) Representative images for multiplexed immunofluorescence staining (DAPI, dark blue; TLR8, red; PD-L1, purple; CD8, yellow; PD-1, orange; foxp3, white; CD56, light blue) in BLCA. Scale bar, 100 μm. (G) The cell intensity of CK+ cells, TLR8+ cells, PD-L1+ cells, CD8+ cells, PD-1+cells, foxp3+ cells and CD56+ cells. (H) Tumor tissue was recognized by CK staining and morphology. Different colors represent different distance from tumor cells. (I) The distance of mutual neighbors was represented by white lines. scale bars, 200 μm. (J) The scatter plot shows the number of total cells at different locations from the tumor cells. (K) The intensity of TLR8+ cells (left image). The scatter plot shows the number of TLR8+ cells at different locations from the tumor cells. (L) The scatter plot shows the number of PD-L1+ cells at different locations from TLR8+ cells. (M) The scatter plot shows the number of TLR8+CK+ cells, and TLR8+ PD-L1+ cells.