IL1 beta Antibody - #DF6251

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产品: IL1 beta 抗体
货号: DF6251
描述: Rabbit polyclonal antibody to IL1 beta
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Horse, Rabbit
蛋白号: P01584
RRID: AB_2838217

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IL1 beta Antibody detects endogenous levels of total IL1 beta.
RRID:
AB_2838217
引用格式: Affinity Biosciences Cat# DF6251, RRID:AB_2838217.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Catabolin; H1; IL 1; IL 1 beta; IL-1 beta; IL1 BETA; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1 beta; Interleukin-1 beta; OAF; OTTHUMP00000162031; Preinterleukin 1 beta; Pro interleukin 1 beta;

抗原和靶标

免疫原:

A synthesized peptide derived from human IL1 beta, corresponding to a region within the internal amino acids.

基因/基因ID:
IL1B(3553)
描述:
The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1beta is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1beta is a good indicator of caspase-1 activity.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Immune diseases > Graft-versus-host disease.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

文献引用

1). Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype. Cell Death & Disease, 2020 (PubMed: 32415059) [IF=8.1]

Application: WB    Species: mouse    Sample: hippocampus

Fig.S1|The levels of pro-IL1β, IL-18, ASC, and cleaved caspase-1 p10 were increased,and pretreatment with MCC950 decreased the expression of these proteins (Figs. 1c and S1C).
2). Paeoniflorin mitigates MMP-12 inflammation in silicosis via Yang-Yin-Qing-Fei Decoction in murine models. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38669965) [IF=6.7]
3). Highly Acylated Anthocyanins from Purple Sweet Potato ( Ipomoea batatas L.) Alleviate Hyperuricemia and Kidney Inflammation in Hyperuricemic Mice: Possible Attenuation Effects on Allopurinol. Journal of Agricultural and Food Chemistry, 2019 (PubMed: 31091873) [IF=5.7]

Application: WB    Species: mouse    Sample: renal

Figure 4.| Western blot of HAA-PSP, allopurinol, and their combination on protein expression for TNF-α, TGF-β1, IL-6, IL-1β, ICAM-1, COX-2,and NF-κB p65 in renal tissue of hyperuricemic mice. The contents of target proteins were normalized to GAPDH. Values are expressed as the means ± SD (n = 8). Different letters marked above the bars are significantly different by an ANOVA multiple test (p < 0.05).
4). Quercetin Alleviates Pulmonary Fibrosis in Mice Exposed to Silica by Inhibiting Macrophage Senescence. Frontiers in pharmacology, 2022 (PubMed: 35959439) [IF=5.6]

Application: WB    Species: Mouse    Sample:

FIGURE 7. Elevated fibrotic and SASP protein expression in senescent macrophages. (A) IL-1β, IL-6, TNF-α and TGF-β1 expressions by western blot. (B) MMP2, MMP9 and MMP12 expressions by western blot. (C) Immunofluorescence staining for TGF-β1. (D) Immunofluorescence staining for MMP12. Data are presented as the mean ± SD. n = 3 per group.
5). Effect of Sex Differences in Silicotic Mice. International Journal of Molecular Sciences, 2022 (PubMed: 36430681) [IF=5.6]

Application: WB    Species: Mouse    Sample: lung tissues

Figure 4 Western blot of CD68, COL I, vimentin, and α-SMA in male and female mice exposed to silica for two, four, six weeks, and the control groups. (A) Immunohistochemical staining was used to detect the expression of CD68 in the mouse lung tissues, arrow: CD68-positive cells (brown) (scale bar = 50 µm). (B–G) Expression levels of Col I, α-SMA, vimentin, IL 6 and IL 1β in the lungs of mice were measured using the western blot. Data are presented as the mean ± SD, n = 5 per group ((B,C)—2W, (D,E)—4W, (F,G)—6W).
6). Thalidomide Alleviates Pulmonary Fibrosis Induced by Silica in Mice by Inhibiting ER Stress and the TLR4-NF-κB Pathway. International Journal of Molecular Sciences, 2022 (PubMed: 35628464) [IF=5.6]

Application: WB    Species: Mice    Sample: MH-S cells

Figure 5 Silica activated the ER stress, TLR4-NF-κB pathway as well as inflammatory response in macrophages. (A–M) Expression levels of p-PERK, p-eIF-2α, p-IRE-1α, TLR4, MyD88, p-IκBα, p-NF-κB, TNF-α, IL-6, and IL-1β in MH-S cells treated with SiO2 at different doses measured by Western blot. * Compared with control group, p < 0.05. Data are presented as the mean ± SD, n = 3 per group.
7). Nr2e1 deficiency aggravates insulin resistance and chronic inflammation of visceral adipose tissues in a diet-induced obese mice model. LIFE SCIENCES, 2021 (PubMed: 33915130) [IF=5.2]

Application: WB    Species: mice    Sample: adipose tissues

Fig. 5. Nr2e1 deficiency exacerbated inflammation in EAT. Comparison of the epididymal fat weight (A). The ratio of epididymal fat weight to the total body weight (B). The mRNA expression of F4/80 (C). The mRNA and protein expressions of IL-6, IL-1β, TNF-α and MCP-1 (E, F). The protein levels of p-IKKβ/IKKβ, p-P65/P65 (G, H). The data are expressed as means ± SEM. *P < 0.05, **P < 0.01. ns: not significant.
8). Shuxuetong injection inhibits pyroptosis in acute ischemic stroke via CD44/NLRP3/GSDMD signal. Journal of ethnopharmacology, 2025 (PubMed: 40074097) [IF=4.8]
9). Thalidomide Attenuates Skin Lesions and Inflammation in Rosacea-Like Mice Induced by Long-Term Exposure of LL-37. Drug design, development and therapy, 2021 (PubMed: 36483458) [IF=4.7]

Application: WB    Species: Mouse    Sample:

Figure 5 Western blot of TNF-α and IL-1β protein expression in dorsal skin. Data are presented as the mean ± SD, n=3 for each group.
10). Modified 5-aminolevulinic acid photodynamic therapy induces cutaneous squamous cell carcinoma cell pyroptosis via the JNK signaling pathway. Biochimica et biophysica acta. Molecular cell research, 2024 (PubMed: 37805058) [IF=4.6]
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