Hexokinase 1 Antibody - #DF6199

Fig. 7 Crocetin reduced E3 ligase RNF146 mediated HK-I ubiquitination and proteasomal degradation. A Protein expression of HK-I in SHSY-5Y cells upon MNNG and crocetin-treatment. Cells were pretreated with concentrations of crocetin (25, 50, 100 μM) for 1 h before 4 h-MNNG (100 μM) stimulation. B Group quantification of (A) from three independent experiments. C mRNA expression of HK-I measured using qRT-PCR analysis. Cells were pretreated with concentrations of crocetin (25, 50, 100 μM) for 1 h before MNNG (100 μM) stimulation for 4 h. mRNA expression was normalized to GAPDH mRNA. D Protein expression of HK-II in SHSY-5Y cells upon MNNG and crocetin-treatment. E PAR, ubiquitination (Ub), and interaction with ubiquitin ligase RNF146 of HK-I protein in SHSY-5Y cells upon MNNG and crocetin-treatment. F–H Group quantification of Ub (F), PAR (G), and RNF146 (H) in (E). Data represent the mean ± SD from three independent experiments. I Expression of HK-I protein in SHSY-5Y cells upon RNF146-knock down (siRNA). J Group quantification of (I). Data represent the mean ± SD from three independent experiments. K Effect of RNF146 knock down (siRNA) on the PARylation and ubiquitination level of HK-I. L Docking analysis between RNF146 and PARylated HK-I. HK-I was represented in green and RNF146 shown in gray. The interaction types of residue pairs between RNF146 and HK-I were shown in detail. Significance was determined by one-way ANOVA. *p