Bim Antibody - #DF6093

Figure 3 GSK2606414 reduced the activation of caspase-3 via mitochondria-dependent apoptosis. (a) Representative western blots showed that GSK2606414 reduced the expression of CHOP, Bim, and cleaved caspase-3 in hippocampal tissue. The pooled data from three mice for each group are summarized in (b). (c) The western blot results showed that the expression of Bax and Bak in the mitochondria was obviously increased and that this effect was accompanied by a reduction in cytochrome c. These effects were prevented by the administration of GSK2606414. (d) Statistical data from three animals for each group are summarized. (e) Representative western blots showing that the cytoplasmic expression of Bax and Bak was obviously reduced and that of cytochrome C was significantly increased. GSK2606414 increased the expression of Bax and Bak and reduced cytochrome C. (f) Statistical data from three animals for each group are summarized. (g) The morphology of mitochondria in the hippocampal CA1 region in the (i) control group, (ii) GSK2606414 group, (iii) IH group, and (iv) IH + GSK2606414 group. In the control and GSK2606414 groups, intact mitochondria (indicated by arrowheads) with clear cristae were found. Much fewer cristae were found in the mitochondria from the IH group. GSK2606414 treatment preserved more intact mitochondria with discernable cristae. Scale bar: 0.25 μm. (h) The mitochondrial membrane potential (JC-1 fluorescence intensity ratio) was impaired by IH treatment and rescued by GSK2606414 treatment. ∗P < 0.05; ∗∗P < 0.01; ns: not significant.

Figure 3:| GSK2606414 reduced the activation of caspase-3 via mitochondria-dependent apoptosis. (a) Representative western blots showed that GSK2606414 reduced the expression of CHOP, Bim, and cleaved caspase-3 in hippocampal tissue.

Figure 3 GSK2606414 reduced the activation of caspase-3 via mitochondria-dependent apoptosis. (a) Representative western blots showed that GSK2606414 reduced the expression of CHOP, Bim, and cleaved caspase-3 in hippocampal tissue. The pooled data from three mice for each group are summarized in (b). (c) The western blot results showed that the expression of Bax and Bak in the mitochondria was obviously increased and that this effect was accompanied by a reduction in cytochrome c. These effects were prevented by the administration of GSK2606414. (d) Statistical data from three animals for each group are summarized. (e) Representative western blots showing that the cytoplasmic expression of Bax and Bak was obviously reduced and that of cytochrome C was significantly increased. GSK2606414 increased the expression of Bax and Bak and reduced cytochrome C. (f) Statistical data from three animals for each group are summarized. (g) The morphology of mitochondria in the hippocampal CA1 region in the (i) control group, (ii) GSK2606414 group, (iii) IH group, and (iv) IH + GSK2606414 group. In the control and GSK2606414 groups, intact mitochondria (indicated by arrowheads) with clear cristae were found. Much fewer cristae were found in the mitochondria from the IH group. GSK2606414 treatment preserved more intact mitochondria with discernable cristae. Scale bar: 0.25 μm. (h) The mitochondrial membrane potential (JC-1 fluorescence intensity ratio) was impaired by IH treatment and rescued by GSK2606414 treatment. ∗P < 0.05; ∗∗P < 0.01; ns: not significant.

Figure 2.| Quxie capsule (QX) inhibited cell proliferation and induced apoptosis in tumor tissues. (A) Ki-67 staining of tumor sections obtained from mice treated with (a) vehicle control or (b) QX. Quantification of Ki-67-positive cells in the tumor sections (c). (B)TUNEL staining of tumor sections obtained from mice treated with (a) vehicle control or (b) QX. Quantification of TUNEL-positive cells in the tumor sections (c). (C) Western blotting of proapoptotic proteins Bim, FasL, and cleaved caspase-3 expression in tumor tissues of QX-treated mice or vehicle control-treated mice. Data are presented as mean ± SD. *P < .05, **P < .01 versus vehicle control.