PGC1 alpha Antibody - #AF5395

Fig. 2. Peroxisome proliferator γ-activated receptor coactivator 1-α (PGC-1α) was involved in NaAsO2-induced hepatic IR and ferroptosis. (A–C) Relative PGC-1α protein and gene expression in rat livers. (D–F) The effect of PGC-1α on total and phosphorylated AKT and GSK3β expression. Cells were transfected with PGC-1α before treatment with 4 μM NaAsO2 for 48 h. Insulin was applied for 10 min before the cells were collected. (G) The effect of PGC-1α on insulin-stimulated glucose uptake. (H–J) The effect of PGC-1α on ACLS4 and GPX4 expression. (K, L) The effect of PGC-1α on Fe2+ was measured by FerroOrange staining (scale bar = 20 μm). (M, N) The effect of PGC-1α on cell lipid reactive oxygen species (ROS) measured with a lipid peroxidation fluorescent probe (scale bar = 20 μm). **P < 0.01 versus the control; #P < 0.05 and ##P < 0.01 versus the NaAsO2 (n = 3).

Fig. 3: High expression levels of S100a8/a9 inhibit viability of endothelial cells via induction of metabolic disorders. A Five types of endothelial cells were showed on the UMAP plot; B The sankey diagram showed the percentages of five types of endothelial cells in sham and CLP groups; C. The volcano map showed the noticeably upregulated genes in capillary endothelial cells; D, E The mRNA expression levels of DUSP1 detected by RT-qPCR in cell and animal experiments (n = 6 in each group); F The extent of MAPK negative feedback was evaluated by GSVA enrichment analysis; G Western blot analysis was used to assess Erk signaling pathway in lung tissues from mice (n = 6 in each group); H, I The viability of endothelial cells was evaluated by CCK8 (n = 3 in each group); J The protein expression levels of Erk signaling pathway were assessed by Western blot (n = 6 in each group); K Metabolic pathways in endothelial cells from mice were evaluated by GSVA enrichment analysis; L Heatmap showed the expression levels of mitochondrial complexes-related genes in endothelial cells from mice. M The significantly downregulated cell components were showed on the dot plot; N The protein expression levels of mitochondrial complexes were assessed by Western blot (n = 6 in each group); O Corrplot R package was used to evaluate the correlation between NRF1 and complex I-related genes in every subcluster of endothelial cell; P, Q The mRNA levels of NRF1 and NDUFA3 were measured by RT-qPCR in cell and animal experiments (n = 12 in each group), and the correlation between NRF1 and NDUFA3 was shown on the correlation curve (n = 24). Each bar showed means ± SEM. Unpaired t-test was used for the comparison between two groups. Comparison among three or more groups was analyzed by one-way ANOVA. *p 

Figure 6. Changes in mitochondrial-related indexes after PARK7 silencing. (A) Changes in mitochondrial synthesis proteins in APAP-treated L02 cells with and without PARK7 silencing and statistical analysis, N = 3. * p < 0.05 vs. ShNC group, ** p < 0.01 vs. ShNC group, # p < 0.05vs. ShNC+APAP group, ## p < 0.01 vs. ShNC+APAP group. (B) WB and statistical analysis of the mitochondrial synthetic proteins PGC-1α, NRF1 and TFAM in the liver tissue of mice with or without APAP intervention with PARK7 knockdown, N = 3, * p < 0.05 vs. WT group, ** p < 0.01 vs. WT group, ## p < 0.01 vs. WT+APAP group. (C,D) Changes in mitochondrial respiratory function and glycolytic function under APAP intervention with or without PARK7 silencing, N = 3. * p < 0.05 vs. shNC group, ** p < 0.01 vs. shNC group, # p < 0.05 vs. shNC+APAP group, ## p < 0.01 vs. shNC+APAP group.

Fig. 3 Effect of maternal HMSeBA supplementation during gestation on the expression of muscle fiber type-related genes in the LD muscle of offspring. A The expression of muscle fiber type-related genes in newborn piglets (n = 10). B The percentage of muscle fiber type in newborn piglets (n = 10). C The protein levels of slow MyHC, fast MyHC and PGC-1α in newborn piglets. D Quantification for proteins of newborn piglets. E The expression of muscle fiber type-related genes in weaned piglets (n = 6). F The percentage of muscle fiber type in weaned piglets (n = 6). G The expression of slow MyHC, fast MyHC and PGC-1α proteins in weaned piglets. H Quantification for proteins of weaned piglets. MyHC I, myosin heavy chain type1; MyHC IIa, myosin heavy chain type 2a; MyHC IIb, myosin heavy chain type 2b; MyHC IIx, myosin heavy chain type 2x; PGC-1a, peroxisome proliferator-activated receptor gamma coactivator-1 alpha; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Data are presented as means ± SE. a,b,cP