PGC1 Antibody - #AF5395

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产品: PGC1 抗体
货号: AF5395
描述: Rabbit polyclonal antibody to PGC1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 100~150 kD; 91kD,113kD(Calculated).
蛋白号: Q9UBK2 | Q86YN6
RRID: AB_2837880

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
克隆:
Polyclonal
特异性:
PGC1 Antibody detects endogenous levels of total PGC1 alpha/beta.
RRID:
AB_2837880
引用格式: Affinity Biosciences Cat# AF5395, RRID:AB_2837880.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

L PGC 1alpha; LEM6; Ligand effect modulator 6; Peroxisome proliferative activated receptor gamma coactivator 1 alpha; Peroxisome proliferative activated receptor gamma coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC-1-alpha; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR-gamma coactivator 1-alpha; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC1; PPARGC1A; PRGC1_HUMAN; PPARGC1B, PERC, PGC1, PGC1B, PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-beta;

抗原和靶标

免疫原:
Uniprot:

Q9UBK2(PRGC1_HUMAN) >>Visit HPA database.

Q86YN6(PRGC2_HUMAN) >>Visit HPA database.

基因/基因ID:
PPARGC1A(10891) | PPARGC1B(133522)
表达:
Q9UBK2 PRGC1_HUMAN:

Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.

Q86YN6 PRGC2_HUMAN:

Ubiquitous with higher expression in heart, brain and skeletal muscle.

描述:
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis.
序列:
MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR

MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSETEPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAPEKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQDVLCDVSKPYRLATPVYASLTPRSRPRPPKDSQASPGRPSSVEEVRIAASPKSTGPRPSLRPLRLEVKREVRRPARLQQQEEEDEEEEEEEEEEEKEEEEEWGRKRPGRGLPWTKLGRKLESSVCPVRRSRRLNPELGPWLTFADEPLVPSEPQGALPSLCLAPKAYDVERELGSPTDEDSGQDQQLLRGPQIPALESPCESGCGDMDEDPSCPQLPPRDSPRCLMLALSQSDPTFGKKSFEQTLTVELCGTAGLTPPTTPPYKPTEEDPFKPDIKHSLGKEIALSLPSPEGLSLKATPGAAHKLPKKHPERSELLSHLRHATAQPASQAGQKRPFSCSFGDHDYCQVLRPEGVLQRKVLRSWEPSGVHLEDWPQQGAPWAEAQAPGREEDRSCDAGAPPKDSTLLRDHEIRASLTKHFGLLETALEEEDLASCKSPEYDTVFEDSSSSSGESSFLPEEEEEEGEEEEEDDEEEDSGVSPTCSDHCPYQSPPSKANRQLCSRSRSSSGSSPCHSWSPATRRNFRCESRGPCSDRTPSIRHARKRREKAIGEGRVVYIQNLSSDMSSRELKRRFEVFGEIEECEVLTRNRRGEKYGFITYRCSEHAALSLTKGAALRKRNEPSFQLSYGGLRHFCWPRYTDYDSNSEEALPASGKSKYEAMDFDSLLKEAQQSLH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
80
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBK2,Q86YN6 作为底物

Site PTM Type Enzyme
T178 Phosphorylation Q13131 (PRKAA1)
S208 Phosphorylation
S246 Phosphorylation
T263 Phosphorylation Q16539 (MAPK14)
S266 Phosphorylation Q16539 (MAPK14)
S273 Phosphorylation
K278 Acetylation
S285 Phosphorylation
T295 Phosphorylation
T299 Phosphorylation Q16539 (MAPK14)
S313 Phosphorylation
K347 Acetylation
K360 Acetylation
K360 Ubiquitination
S416 Phosphorylation
S417 Phosphorylation
K442 Ubiquitination
K464 Ubiquitination
K480 Ubiquitination
S539 Phosphorylation
S571 Phosphorylation P31751 (AKT2) , P31749 (AKT1)
S616 Phosphorylation
Y634 Phosphorylation
S636 Phosphorylation
Y717 Phosphorylation
T721 Phosphorylation
Y722 Phosphorylation
K758 Ubiquitination
S768 Phosphorylation
K777 Ubiquitination
K779 Ubiquitination
S782 Phosphorylation
S787 Phosphorylation
K790 Ubiquitination
Site PTM Type Enzyme
S239 Phosphorylation
S256 Phosphorylation
S263 Phosphorylation
K303 Ubiquitination
K314 Ubiquitination
K357 Ubiquitination
S384 Phosphorylation
S524 Phosphorylation
S570 Phosphorylation P31749 (AKT1)
S638 Phosphorylation
S643 Phosphorylation
T647 Phosphorylation
S711 Phosphorylation
S854 Phosphorylation
S856 Phosphorylation
S859 Phosphorylation
S865 Phosphorylation
Y943 Phosphorylation
T947 Phosphorylation
Y948 Phosphorylation
Y987 Phosphorylation
Y990 Phosphorylation
S992 Phosphorylation

研究背景

功能:

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

翻译修饰:

Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.

Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.

Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.

细胞定位:

Nucleus. Nucleus>PML body.

Nucleus.

Cytoplasm. Nucleus.

Nucleus. Nucleus>PML body.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.

亚基结构:

Homooligomer. Interacts with MYBBP1A; inhibits MYBBP1A transcriptional activation (By similarity). Interacts with PRDM16, LPIN1 and PML (By similarity). Interacts (via LXXLL motif) with RORA and RORC (via AF-2 motif); activates RORA and RORC transcriptional activation (By similarity). Interacts with LRPPRC. Interacts with RNF34 (via RING-type zinc finger).

功能:

Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous with higher expression in heart, brain and skeletal muscle.

亚基结构:

Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA. Interacts with PRDM16 (By similarity). Interacts with estrogen receptor alpha/ESR1.

蛋白家族:

Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Cathepsin S activity controls chronic stress-induced muscle atrophy and dysfunction in mice. Cellular and Molecular Life Sciences (PubMed: 37589754) [IF=8.0]

Application: WB    Species: Mouse    Sample:

Fig. 4 CTSS deficiency ameliorated stress-related anabolic and catabolic molecular alterations. a–e: Representative immunoblotting images and quantitative data for CTSS, IGF-1, IRS-2, p-PI3K, p-Akt, p-mTOR, p-FoxO1α, MuRF-1, MAFbx1, PGC-1α, PPAR-γ, C-caspase-3, and Bcl-2 in GAS muscles at Day 14 after stress (n = 3). Data are mean ± SEM, and p-values were determined by a one-way ANOVA followed by Bonferroni post hoc tests (b–e). CW: CTSS+/+ control mice, CK: CTSS−/− control mice, SW: 14-day-stressed CTSS+/+ mice, SK: 14-day-stressed CTSS−/− mice. *p 
2). Xuanfei Baidu formula alleviates impaired mitochondrial dynamics and activated NLRP3 inflammasome by repressing NF-κB and MAPK pathways in LPS-induced ALI and inflammation models. Phytomedicine (PubMed: 36423572) [IF=7.9]
3). β-patchoulene improves lipid metabolism to alleviate non-alcoholic fatty liver disease via activating AMPK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY (PubMed: 33341045) [IF=7.5]

Application: WB    Species: Human    Sample: L02 cell

Fig. 6. β-PAE promotes the expression of hepatic lipid oxidation-related proteins and genes in HFD-fed rats. (A–G) Western blot analysis on the expression of SIRT1, PGC-1α, PPARα, FGF21, CPT-1a and ACOX1; (H–K) The mRNA expression of SIRT1, PPARα, CPT-1a and ACOX1. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.
4). Resveratrol and its derivative pterostilbene attenuate oxidative stress-induced intestinal injury by improving mitochondrial redox homeostasis and function via SIRT1 signaling. Free radical biology & medicine (PubMed: 34648904) [IF=7.4]
5). A novel HSF1 activator ameliorates non‐alcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation. BRITISH JOURNAL OF PHARMACOLOGY (PubMed: 34783017) [IF=7.3]

Application: WB    Species: Human    Sample: HepG-2 cells

FIGURE 3 The PGC-1ɑ is an important target gene of heat shock factor 1 (HSF1). (a) Scatter plot of the log2 (fold change) versus the mean read count per gene generated using ChIP-seq expression profiling data. The dots represent differentially expressed genes according to DEseq2 analysis. Three replicates were included in each group. For the bioinformatics analyses displayed in (a)–(e), 916 high-confidence sites were defined by the following criteria: a fold change (SYSU-3d vs. palmitic acid (PA)) according to DEsp 

Application: IF/ICC    Species: Human    Sample: HepG-2 cells

FIGURE 3 The PGC-1ɑ is an important target gene of heat shock factor 1 (HSF1). (a) Scatter plot of the log2 (fold change) versus the mean read count per gene generated using ChIP-seq expression profiling data. The dots represent differentially expressed genes according to DEseq2 analysis. Three replicates were included in each group. For the bioinformatics analyses displayed in (a)–(e), 916 high-confidence sites were defined by the following criteria: a fold change (SYSU-3d vs. palmitic acid (PA)) according to DEsp 
6). Maternal organic selenium supplementation during gestation enhances muscle fiber area and muscle fiber maturation of offspring in porcine model. Journal of Animal Science and Biotechnology (PubMed: 36329544) [IF=7.0]

Application: WB    Species: piglets    Sample:

Fig. 3 Effect of maternal HMSeBA supplementation during gestation on the expression of muscle fiber type-related genes in the LD muscle of offspring. A The expression of muscle fiber type-related genes in newborn piglets (n = 10). B The percentage of muscle fiber type in newborn piglets (n = 10). C The protein levels of slow MyHC, fast MyHC and PGC-1α in newborn piglets. D Quantification for proteins of newborn piglets. E The expression of muscle fiber type-related genes in weaned piglets (n = 6). F The percentage of muscle fiber type in weaned piglets (n = 6). G The expression of slow MyHC, fast MyHC and PGC-1α proteins in weaned piglets. H Quantification for proteins of weaned piglets. MyHC I, myosin heavy chain type1; MyHC IIa, myosin heavy chain type 2a; MyHC IIb, myosin heavy chain type 2b; MyHC IIx, myosin heavy chain type 2x; PGC-1a, peroxisome proliferator-activated receptor gamma coactivator-1 alpha; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Data are presented as means ± SE. a,b,cP 
7). Thiamine Supplementation Alleviates Lipopolysaccharide-Triggered Adaptive Inflammatory Response and Modulates Energy State via Suppression of NFκB/p38 MAPK/AMPK Signaling in Rumen Epithelial Cells of Goats. Antioxidants (PubMed: 36290775) [IF=7.0]

Application: WB    Species: Goat    Sample: RECs

Figure 6 Thiamine (THI) supplementation modulates energy metabolism disturbance induced by lipopolysaccharide (LPS) in RECs. (A) ATP content for different treatments. (B) The expression of AMPKα1, AMPKα2, SIRT1, and PGC1α genes with different treatments. (C) Representative Western blots for AMPKα, phosphorylated AMPKα (p-AMPKα), SIRT1, and PGC1α protein levels with different treatments. (D–G) Immunoblotting and measurement of intensity. Protein levels were normalized to the respective abundance of β-actin. Data are presented as means ± SEM (standard error of the mean), n = 3/group (results representative of at least three independent experiments); CON group, no added LPS or THI; THI group, 5 μg/mL THI; LPS group, 5 μg/mL LPS; LPTH group, 5 μg/mL THI and 5 μg/mL LPS. * denotes p < 0.05, significant difference.
8). ApoE deficiency promotes non-alcoholic fatty liver disease in mice via impeding AMPK/mTOR mediated autophagy. LIFE SCIENCES (PubMed: 32304762) [IF=6.1]

Application: WB    Species: mouse    Sample: liver

Fig. 5. |Mitochondrial function were downregulated in ApoE−/−-HFD mice. (A–D)Representative immunoblots and relative protein levels of PGC1α and NRF1. (E–F)Hepatic expression and relative flourescent intensity of ROS by immunofluorescent staining.
9). Anti-fatigue effect of Auxis thazard oligopeptide via modulation of AMPK/PGC-1α pathway in mice. Food & Function (PubMed: 35080545) [IF=6.1]
10). Daidzein alleviates doxorubicin-induced heart failure via the SIRT3/FOXO3a signaling pathway. Food & Function (PubMed: 36000402) [IF=6.1]
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