TRAF3 Antibody - #AF5380

Fig. 7.| Tumor necrosis factor receptor-associated factor 3 is the key element for SRA to limit type I IFN response. HepG2.2.15 cells were transfected with TRAF3 siRNA, together with or without SRA siRNA for 48 h. Western blotting was performed to examine the level of cellular HBcAg (A).

Fig. 5. |Scavenger receptor A colocalizes and interacts with signaling transducer TRAF3. (B) Colocalization of SRA with TRAF3 in HepG2.2.15 cells. The cells were cultured on glass coverslips and immunostained with antibodies against SRA and TRAF3, respectively,subsequently analyzed by a confocal microscope (Magnification: 6309).

Fig. 5. Scavenger receptor A colocalizes and interacts with signaling transducer TRAF3. (A) SRA coimmunoprecipitates with TRAF3 in HepG2.2.15 cells. Equal amounts of cell extract were immunoprecipitated with SRA-specific antibody or control IgG. The immune complex was analyzed using anti-TRAF3 antibody (left panel). Besides, TRAF3 was pulled down and analyzed for association with SRA using antiSRA antibody (right panel). Levels of SRA and TRAF3 in the whole cell lysate were also examined. (B) Colocalization of SRA with TRAF3 in HepG2.2.15 cells. The cells were cultured on glass coverslips and immunostained with antibodies against SRA and TRAF3, respectively, subsequently analyzed by a confocal microscope (Magnification: 6309). (C) HepG2 cells were transfected with pHBV1.3 or control plasmid, and equal amounts of protein were used for immunoprecipitation with the SRA-specific antibody. The immunoprecipitates were probed for the presence of TRAF3 and SRA, respectively. (D) TRAF-C domain is essential for the binding of TRAF3 to SRA. HEK293T cells were transfected with HA-tagged SRA and FLAG-tagged full-length TRAF3 or several truncated TRAF3 deletion mutants. Immunoprecipitation was performed using anti-FLAG antibody. The presence of coprecipitated SRA was determined by immunoblotting with the anti-HA antibody. Representative results from three independent experiments are shown.

Figure 4.| Lentiviral vector-mediated overexpression of TLR3 in BV-2 cells. The lentivirus (10 MOI) was incubated with the BV-2 cells for 72 h. (A) The expression of GFP was observed with a fluorescence microscope after lentivirus was introduced into the BV-2 cells. (B) The mRNA levels of TLR3 and its downstream molecules were measured by RT-PCR. (C) The protein levels were detected by western blotting. Data are shown as the mean ± SD of three independent experiments of triplicate samples.**P < 0.01 for lentivirus-up vs. normal; *P < 0.05 for lentivirus-up vs. normal (by Student’s t-test).

Figure 7 Treatment with ICA and PNS reduced MIPT3 and increased Oasl1 and TCHP protein levels. N=6 in each group. *P < 0.05 compared the control group, #P < 0.05 compared with the AD group.

Figure 4. The protein expression of TLR3 and its downstream protein in brain tissues of mice.