SOCS1 Antibody - #AF5378

Figure 3 The SOCS1 3′-UTR is a tRF-23 target (A) A schematic of the predicted tRF-23 binding site in the SOCS1 3′-UTR, with residues that were selectively mutated highlighted in red. (B) Luciferase activity analyses indicated that transfection with tRF-23 reduced WT but not mutated SOCS1 3′-UTR reporter construct luciferase activity by 40%. Data are presented as mean ± SD (n = 3 independent experiments, each with two technical replicates per independent sample). (C and D) The effects of tRF-23 on SOCS1 expression on day 14 of hBMSC osteogenesis were analyzed. Data are presented as the mean ± SD (n = 3 independent experiments with two technical replicates per independent sample). (E) SOCS1 knockdown enhanced matrix mineralization and positive (blue-violet) staining. Scale bars: 50 μm. Matrix mineralization and ALP activity were quantified based on absorbance. Data are presented as the mean ± SD (n = 3 independent experiments with two technical replicates per independent sample). (F and G) RUNX2, OCN, and ALP levels on day 14 of hBMSC osteogenesis were detected via qPCR and western blotting. Data are presented as the mean ± SD (n = 3 independent experiments with two technical replicates per independent sample). Statistical analysis was performed using paired two-tailed Student’s t tests. ∗∗p < 0.01 was considered significant. NC, negative control; OE, overexpression; shRNA, short hairpin RNA; ns, not significant.

Figure 6. Knockout of Mer reduced STAT1 activation and SOCS expression after CLP. Representative immunoblots and quantification showing the expression of TLR4 (A), NF-κB (B) was significantly increased in WT and Mer-/- group at day 4 after CLP; however, the expression of phosphorylated STAT1 (p-STAT1) (C), SOCS1 (D), and SOCS3 (E) were significantly decreased at day 4 after CLP. Data are expressed as fold change compared to the Sham + WT group; n = 5 rats per group. *P < 0.05 compared to Sham + WT group,