TRAF6 Antibody - #AF5376

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产品: TRAF6 抗体
货号: AF5376
描述: Rabbit polyclonal antibody to TRAF6
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
蛋白号: Q9Y4K3
RRID: AB_2810280

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TRAF6 Antibody detects endogenous levels of total TRAF6.
RRID:
AB_2810280
引用格式: Affinity Biosciences Cat# AF5376, RRID:AB_2810280.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

E3 ubiquitin-protein ligase TRAF6; Interleukin 1 signal transducer; Interleukin-1 signal transducer; MGC 3310; MGC:3310; MGC3310; OTTHUMP00000232772; OTTHUMP00000232773; RING finger protein 85; RNF 85; RNF85; TNF receptor associated factor 6; TNF receptor-associated factor 6; TNF receptor-associated factor 6, E3 ubiquitin protein ligase; TRAF 6; Traf6; TRAF6_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human TRAF6, corresponding to a region within the internal amino acids.

基因/基因ID:
TRAF6(7189)
描述:
Tumor necrosis factor receptor-associated factor 6 (TRAF6) regulates adaptive immunity, innate immunity and bone metabolism. TRAF6 is a ubiquitin (Ub) ligase that mediates the activation of protein kinases, such as transforming growth factor beta-activated kinase (TAK1) and IκB kinase (IKK)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

文献引用

1). Breaking the vicious loop between inflammation, oxidative stress and coagulation, a novel anti-thrombus insight of nattokinase by inhibiting LPS-induced inflammation and oxidative stress. Redox Biology, 2020 (PubMed: 32193146) [IF=10.7]

Application: WB    Species: Mice    Sample: RAW264.7 cells

Fig. 5. NK diminished LPS-induced TLR4 activation likely due to promoting TRL4 proteolysis in RAW264.7 cells. (A) Effect of NK on LPS-induced TLR4 signaling pathways. Cells were pretreated with indicated concentrations of NK for 1 h and then exposed to LPS (0.1 μg/mL) for 12 h. Equal amounts of total cell lysates were loaded and subjected to immunoblot analysis. Data represent the mean ± SD from three independent experiments. (B) NK induced TRL4 degradation via its serine protease activity in RAW264.7 cells. Cells were treated with NK (0.3 FU/mL) for indicated time points with or without PMSF pretreatment for 30 min. Equal amounts of total cell lysates were loaded and subjected to immunoblot analysis. Data represent mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 compared to control group; #P < 0.05, ##P < 0.05 compared to NK 12h group; $ P < 0.05, $$P < 0.05 compared to NK 24h group.
2). The YTHDF1–TRAF6 pathway regulates the neuroinflammatory response and contributes to morphine tolerance and hyperalgesia in the periaqueductal gray. Journal of Neuroinflammation, 2022 (PubMed: 36550542) [IF=9.3]
3). Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis. Chemosphere, 2021 (PubMed: 33297146) [IF=8.1]

Application: WB    Species: Mice    Sample: testes

Fig. 6. F can stimulate downstream proteins in IL-17A signal pathway in the testes (n ¼ 13. Three times repetition). Statistical analysis was carried out using one-way ANOVA with Dunnett’s test. Error bars denote the mean ± s.e.m. *P < 0.05, **P < 0.01.
4). The matrix protein of Newcastle disease virus inhibits inflammatory response through IRAK4/TRAF6/TAK1/NF-κB signaling pathway. International Journal of Biological Macromolecules, 2022 (PubMed: 35872314) [IF=7.7]
5). TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence, 2020 (PubMed: 32420802) [IF=5.5]

Application: WB    Species: Mouse    Sample: BSR-T7/5 cells

Figure 9. rSS1GFP replication is enhanced by inhibiting TIFA/TRAF6/NF-κB signaling pathway later in infection. (A) The mRNA expression levels of TIFA gene in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (B) The protein expression levels of TIFA in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of TIFA were compared with the control GAPDH expression. (C) The subcellular localization of EGFP-M or EGFP-M/NLSm and HA-TIFA in plasmids co-transfected BSR-T7/5 cells. Original magnification was 1 × 200. (D) The effect of different dosage EGFP-M or EGFP-M/NLSm on the expression level of endogenous TIFA in plasmid transfected BSR-T7/5 cells. The relative expression levels of TIFA were compared with the control GAPDH expression. (E) The expression patterns of TIFA, pTIFA, TRAF6, NF-κB p65, pNF-κB p65, and IL-2 in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm at 12 and 24 hpi. The relative expression levels of these proteins were compared with the control GAPDH expression. (F) The effect of TIFA overexpression on the expression of IL-2 and virus titers of rSS1GFP and rSS1GFP-M/NLSm at 12 and 24 hpi. (G) The effect of siRNA-mediated knockdown of TIFA on the expression of IL-2 and virus titers of rSS1GFP and rSS1GFP-M/NLSm at 12 and 24 hpi. (H) The schematic diagram illustrated that the M protein in the cytoplasm inhibited host cell immune response by down-regulating TIFA/TRAF6/NF-κB signaling pathway
6). Lipid nanoparticles of quercetin (QU-Lip) alleviated pancreatic microenvironment in diabetic male rats: The interplay between oxidative stress - unfolded protein response (UPR) - autophagy, and their regulatory miRNA. Life sciences, 2024 (PubMed: 38462227) [IF=5.2]
7). TRAF6 promotes the progression and growth of colorectal cancer through nuclear shuttle regulation NF-kB/c-jun signaling pathway. LIFE SCIENCES, 2019 (PubMed: 31487530) [IF=5.2]
8). Chang-Kang-Fang alleviates diarrhea predominant irritable bowel syndrome (IBS-D) through inhibiting TLR4/NF-κB/NLRP3 pathway. Journal of ethnopharmacology, 2024 (PubMed: 38670405) [IF=4.8]
9). Cornuside protects against ischemic stroke in rats by suppressing the IL-17F/TRAF6/NF-κB pathway via the brain-gut axis. Experimental neurology, 2024 (PubMed: 38169196) [IF=4.6]
10). Transmission of NLRP3-IL-1β Signals in Cerebral Ischemia and Reperfusion Injury: from Microglia to Adjacent Neuron and Endothelial Cells via IL-1β/IL-1R1/TRAF6. Molecular Neurobiology, 2023 (PubMed: 36717480) [IF=4.6]
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