RANTES Antibody - #AF5151

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产品: RANTES 抗体
货号: AF5151
描述: Rabbit polyclonal antibody to RANTES
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P13501
RRID: AB_2837637

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
RANTES Antibody detects endogenous levels of total RANTES.
RRID:
AB_2837637
引用格式: Affinity Biosciences Cat# AF5151, RRID:AB_2837637.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Beta chemokine RANTES; Beta chemokine RANTES precursor; C C motif chemokine 5; CCL 5; CCL5; CCL5_HUMAN; Chemokine (C C motif) ligand 5; Chemokine CC Motif Ligand 5; D17S136E; EoCP; Eosinophil chemotactic cytokine; MGC17164; RANTES(4-68); Regulated upon activation normally T expressed and presumably secreted; SCYA 5; SCYA5; SIS delta; SIS-delta; SISd; Small inducible cytokine A5 (RANTES); Small inducible cytokine A5; Small inducible cytokine subfamily A (Cys Cys) member 5; Small-inducible cytokine A5; T cell specific protein p288; T cell specific RANTES protein; T cell-specific protein P228; T-cell-specific protein RANTES; TCP 228; TCP228;

抗原和靶标

免疫原:

A synthesized peptide derived from human RANTES, corresponding to a region within the internal amino acids.

基因/基因ID:
CCL5(6352)
描述:
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome. Bone research, 2024 (PubMed: 39242551) [IF=14.3]
2). Hyperphosphorylated tau mediates neuronal death by inducing necroptosis and inflammation in Alzheimer’s disease. Journal of Neuroinflammation, 2022 (PubMed: 35971179) [IF=9.3]

Application: WB    Species: Mouse    Sample: HT22 cells

Fig. 2Hyperphosphorylated tau induces cytokine upregulation. A Differentially expressed genes (DEGs) between HT22 cells transfected with vector or TauP301S for 48 h. B Analysis of DEGs in the TauP301S and Tau groups according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Heat map of cytokine genes differentially expressed in HT22 cells transfected with vector or Tau or TauP301S following treatment with DMSO or Nec-1 (30 μM); low expression is shown in blue and high expression in red. D mRNAs from HT22 cells transfected with vector or TauP301S following treatment with DMSO or Nec-1 (30 μM) were extracted and quantified to determine indicated cytokine levels by qPCR. E Cytokine levels in HT22 cells transfected with vector or TauP301S following treatment with DMSO or Nec-1 (30 μM) were analyzed by western blotting with the indicated antibodies. F ROS levels in HT22 cells transfected with vector or TauP301S. G Chemotaxis of pTau-induced cytokines on BV2 cells was analyzed by transwell assays, Scale bars, 100 μm. Data are presented as the mean ± standard error of the mean (SEM) of three experiments, and statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test in D and two-tailed unpaired t test in F, G
3). CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling. Cell Death & Disease, 2020 (PubMed: 32300100) [IF=8.1]

Application: WB    Species: human    Sample: prostate cancer cells

Fig. 3 TAM-secreted CCL5 promoted the invasion of prostate cancer cells and the self-renewal of PCSCs. a, b The reliability of THP1-derived TAMs was validated by analyzing the macrophage surface markers. THP1 monocytes were induced differentiation into macrophages (M0) by 100 ng/ ml PMA treatment for 24 h, which were further transformed into M2 phenotype macrophages (TAMs) by 10 ng/ml IL-4 induction for 72 h. c, d The CM of THP1-derived TAMs significantly promoted EMT, migration and invasion of prostate cancer cells, while CCL5 NA could partly abrogate that. Scale bars represent 200 μm for wound healing assay images and 50 μm for transwell assay images. e The CM of THP1-derived TAMs significantly promoted the self-renewal of PCSCs, while CCL5 NA could partly abrogate that. All values are presented as the mean ± SD. n = 3, *p < 0.05, **p < 0.01.

Application: IF/ICC    Species: human    Sample: prostate cancer cells

Fig. 1 TAMs-derived CCL5 was elevated in prostate cancer and associated with metastasis. a, b The mRNA and protein expression differences of indicated genes between prostate cancer tumor tissues and para-carcinoma tissues (n = 3). c Elisa assay indicated that the blood samples of prostate cancer patients (n = 30) exhibited elevated expression of CCL5 when compared with that of healthy male participants (n = 30). d Prostate cancer patients with higher Gleason grade exhibited an increased CCL5 accumulation in the blood (n = 30). e Tissue immunofluorescence assay suggested that CCL5 and the macrophage marker CD163 were co-localized in both the primary tumor and metastatic lymph node of prostate cancer patients. Scale bar, 10 μm. f TAMs exhibited increased secretion of CCL5 than immature macrophages or prostate cancer cells. CCL5 concentrations in the cell culture supernatants of THP1-derived macrophages (M0), THP1-derived TAMs (M2), DU145 and PC3 cells were measured using Elisa method (n = 10). All values are presented as the mean ± SD. *p < 0.05, **p < 0.01.
4). The myokine CCL5 recruits subcutaneous preadipocytes and promotes intramuscular fat deposition in obese mice. American journal of physiology. Cell physiology, 2024 (PubMed: 38497114) [IF=5.0]
5). A novel long noncoding RNA, TMEM92‐AS1, promotes gastric cancer progression by binding to YBX1 to mediate CCL5. Molecular Oncology, 2021 (PubMed: 33247987) [IF=5.0]

Application: WB    Species: human    Sample: BGC823 and SGC7901 cells

Figure 4 CCL5 was confirmed as a downstream target gene of TMEM92-AS1. (A and B) RT-qPCR and western blot were used to verify changed genes and proteins after TMEM92-AS1 knockdown.

Application: WB    Species: human    Sample: BGC823 cells

Figure 5(C) RT-qPCR and western blot were performed to detect CCL5 expressions after YBX1 knockdown.

Application: WB    Species: human    Sample: BGC823 cells

Figure 5(E) RT-qPCR and western blot were performed to detect the effect of TMEM92-AS1 overexpression and YBX1 knockdown on CCL5 expression.

Application: WB    Species: human    Sample: BGC823 cells

Figure 5 (G) ChIP results of YBX1 of the promoter region of CCL5 after si-TMEM92-AS1 2# in BGC823 cell
6). C-C motif chemokine ligand 5 contributes to radon exposure-induced lung injury by recruiting dendritic cells to activate effector T helper cells. Toxicology, 2025 (PubMed: 39746565) [IF=4.8]
7). ASCT2-mediated glutamine uptake of epithelial cells facilitates CCL5-induced T cell infiltration via ROS-STAT3 pathway in oral lichen planus. International immunopharmacology, 2023 (PubMed: 37116342) [IF=4.8]
8). IFN-γ Activates the TLR4-CCL5 Signaling Through Reducing Arginine Level, Leading to Enhanced Susceptibility of Bovine Mammary Epithelial Cells to Staphylococcus aureus. INFLAMMATION, 2020 (PubMed: 32725514) [IF=4.5]

Application: WB    Species: Cows    Sample: bovine mammary epithelial cells (BMECs)

Fig. 2. IFN-γ promoted the expression of CCL5 and CCL5 antibody inhibited the infection of S. aureus. a DEGs associated with inflammation were screened out: DEGs were screened according to false discovery rate (FDR) ≤ 0.05 and |log2 ratio ≥ 1. Among 12 genes associated with inflammation, seven of the genes were increased and five decreased. b Western blot detection of CCL5 expression in BMECs that were stimulated by IFN-γ. c The expression ratio of CCL5 in (b) was quantified by ImageJ software. d The expression of CCL5 and the adhesion of S. aureus were detected by immunofluorescence after IFN-γ treatment. Green indicated S. aureus, blue indicated the nucleus of BMECs, and anti-SPA referred to S. aureus-specific antibody. e The adhesion of S. aureus to BMECs after treatment with CCL5 antibody. f The invasion of S. aureus to BMECs after treatment with CCL5 antibody. RB-IgG means rabbit IgG. The experiment was repeated three times.

Application: IHC    Species: Cows    Sample: bovine mammary epithelial cells (BMECs)

Fig. 2. IFN-γ promoted the expression of CCL5 and CCL5 antibody inhibited the infection of S. aureus. a DEGs associated with inflammation were screened out: DEGs were screened according to false discovery rate (FDR) ≤ 0.05 and |log2 ratio ≥ 1. Among 12 genes associated with inflammation, seven of the genes were increased and five decreased. b Western blot detection of CCL5 expression in BMECs that were stimulated by IFN-γ. c The expression ratio of CCL5 in (b) was quantified by ImageJ software. d The expression of CCL5 and the adhesion of S. aureus were detected by immunofluorescence after IFN-γ treatment. Green indicated S. aureus, blue indicated the nucleus of BMECs, and anti-SPA referred to S. aureus-specific antibody. e The adhesion of S. aureus to BMECs after treatment with CCL5 antibody. f The invasion of S. aureus to BMECs after treatment with CCL5 antibody. RB-IgG means rabbit IgG. The experiment was repeated three times.
9). RNA demethylase ALKBH5 suppresses tumorigenesis via inhibiting proliferation and invasion and promoting CD8+ T cell infiltration in colorectal cancer. Translational Oncology, 2023 (PubMed: 37224767) [IF=4.5]

Application: WB    Species: Human    Sample: HCT116 and SW620 cells

Fig. 6 ALKBH5 disrupting NF-κB-CCL5 signaling. a-b Relative expression of CCL5 in ALKBH5-overexpressed HCT116 and SW620 cells. c Immunohistochemistry was used to detect the expression of ALKBH5, p65, CCL5, and CD8 in human colorectal cancer tissues, the data represented mean ± SD (n=56). d CCL5 expression and the total and phosphorylation level of p65 and IκB-α after ALKBH5 overexpression were examined by western blot analysis. e SW620 were pretreated with 100 and 200 ng/ml of specific NF-κB activator PMA, and western blot analysis was to assess the expression of CCL5 and p-p65.

Application: IHC    Species: Human    Sample: HCT116 and SW620 cells

Fig. 6 ALKBH5 disrupting NF-κB-CCL5 signaling. a-b Relative expression of CCL5 in ALKBH5-overexpressed HCT116 and SW620 cells. c Immunohistochemistry was used to detect the expression of ALKBH5, p65, CCL5, and CD8 in human colorectal cancer tissues, the data represented mean ± SD (n=56). d CCL5 expression and the total and phosphorylation level of p65 and IκB-α after ALKBH5 overexpression were examined by western blot analysis. e SW620 were pretreated with 100 and 200 ng/ml of specific NF-κB activator PMA, and western blot analysis was to assess the expression of CCL5 and p-p65.
10). TLR3 as an emerging molecule facilitating pyroptosis in the context of rheumatoid arthritis: A study combined bioinformatics and experimental validation. Cytokine, 2025 (PubMed: 39884182) [IF=3.7]
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