RPL34 Antibody - #DF3708

Figure 3 Proteomic analysis reveals that DKC1 elicits ribosomal protein expression. A) Volcano plot of differentially expressed proteins obtained from proteomic analysis of triplicate samples of DKC1 knockdown DLD‐1 cells and control cells. A total of 114 downregulated proteins and 58 upregulated proteins were included. B,C) GO enrichment analysis for the cellular component category (B) and KEGG enrichment analysis (C) of differentially expressed proteins. D) Comparison of the mRNA abundance of 28 ribosomal proteins measured by qRT‐PCR and their protein levels measured by proteomic analysis of DKC1 knockdown DLD‐1 cells and control cells (All proteins with P < 0.05). E) qRT‐PCR assays evaluating the mRNA levels of the indicated genes in control and DKC1 knockdown DLD‐1 cells with or without enforced expression of wild‐type DKC1 or the DKC1 mutant (D125A) from three independent experiments (mean ± SD). F) Immunoblots assessing the abundance of the indicated ribosomal proteins in control and DKC1 knockdown DLD‐1 and HCT116 cells with or without enforced expression of wild‐type DKC1 or D125A and PF‐treated DLD‐1 and HCT116 cells (48 h). E.V: empty vector. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significance ((D) two‐sided Student's t‐test, (E) one‐way ANOVA with Bonferroni correction).

Figure 8. rSS1GFP infection affects the expression of cellular translation, posttranslational modification and trafficking-associated proteins. (A) The heatmap of representative 20 DEPs related to “Translation, ribosomal structure and biogenesis” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Translation, ribosomal structure and biogenesis” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “Posttranslational modification, protein turnover, chaperones” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “Posttranslational modification, protein turnover, chaperones” are analyzed by the STRING software. (E) The heatmap of representative 20 DEPs related to “Intracellular trafficking, secretion, and vesicular transport” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (F) The protein-protein interactions of the DEPs related to “Intracellular trafficking, secretion, and vesicular transport” are analyzed by the STRING software. (G) The mRNA expression levels of six selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (H) The protein expression levels of six DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of six DEPs were compared with the control GAPDH expression.