Mnk1 Antibody - #AF0373

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产品: Mnk1 抗体
货号: AF0373
描述: Rabbit polyclonal antibody to Mnk1
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 51kDa; 51kD(Calculated).
蛋白号: Q9BUB5
RRID: AB_2833534

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Mnk1 Antibody detects endogenous levels of total Mnk1.
RRID:
AB_2833534
引用格式: Affinity Biosciences Cat# AF0373, RRID:AB_2833534.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; mknk1; MKNK1_HUMAN; MNK 1; Mnk1;

抗原和靶标

免疫原:
Uniprot:

Q9BUB5(MKNK1_HUMAN) >>Visit HPA database.

基因/基因ID:
MKNK1(8569)
表达:
Q9BUB5 MKNK1_HUMAN:

Ubiquitous.

描述:
Mnk1 a CAM kinase of the MAPKKAPK family that can regulate translation. Following activation by MAP kinases, Mnk1 phosphorylates eIF4E at Ser209, increasing its affinity for the 7-methylguanosine-containing mRNA cap. Two MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for its kinase activity. Three alternatively spliced human isoforms have been described.
序列:
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BUB5 作为底物

Site PTM Type Enzyme
S39 Phosphorylation Q13177 (PAK2)
Y60 Phosphorylation
S68 Phosphorylation
S148 Phosphorylation
S191 Phosphorylation
S221 Phosphorylation
S226 Phosphorylation
S237 Phosphorylation
T250 Phosphorylation Q16539 (MAPK14)
T255 Phosphorylation Q16539 (MAPK14) , P28482 (MAPK1)
K332 Ubiquitination
K338 Ubiquitination
K353 Ubiquitination
T385 Phosphorylation P27361 (MAPK3)
S393 Phosphorylation
S413 Phosphorylation
S437 Phosphorylation
R455 Methylation
R459 Methylation
S460 Phosphorylation

翻译修饰 - Q9BUB5 作为激酶

Substrate Site Source
O43597 (SPRY2) S112 Uniprot
O43597 (SPRY2) S121 Uniprot
P06730 (EIF4E) S209 Uniprot
P09651 (HNRNPA1) S192 Uniprot
P09651 (HNRNPA1) S362 Uniprot
P09651 (HNRNPA1) S363 Uniprot
P09651 (HNRNPA1) S364 Uniprot
P23246 (SFPQ) S8 Uniprot
P23246 (SFPQ) S283 Uniprot
P47712 (PLA2G4A) S727 Uniprot
Q9NP80 (PNPLA8) S511 Uniprot

研究背景

功能:

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

翻译修饰:

Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.

细胞定位:

Cytoplasm.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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