Mnk1 Antibody - #AF0373
产品: | Mnk1 抗体 |
货号: | AF0373 |
描述: | Rabbit polyclonal antibody to Mnk1 |
应用: | WB IF/ICC |
反应: | Human, Mouse |
预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 51kDa; 51kD(Calculated). |
蛋白号: | Q9BUB5 |
RRID: | AB_2833534 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0373, RRID:AB_2833534.
展开/折叠
MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; mknk1; MKNK1_HUMAN; MNK 1; Mnk1;
抗原和靶标
- Q9BUB5 MKNK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BUB5 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S39 | Phosphorylation | Q13177 (PAK2) | Uniprot |
Y60 | Phosphorylation | Uniprot | |
S68 | Phosphorylation | Uniprot | |
S148 | Phosphorylation | Uniprot | |
S191 | Phosphorylation | Uniprot | |
S221 | Phosphorylation | Uniprot | |
S226 | Phosphorylation | Uniprot | |
S237 | Phosphorylation | Uniprot | |
T250 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
T255 | Phosphorylation | Q16539 (MAPK14) , P28482 (MAPK1) | Uniprot |
K332 | Ubiquitination | Uniprot | |
K338 | Ubiquitination | Uniprot | |
K353 | Ubiquitination | Uniprot | |
T385 | Phosphorylation | P27361 (MAPK3) | Uniprot |
S393 | Phosphorylation | Uniprot | |
S413 | Phosphorylation | Uniprot | |
S437 | Phosphorylation | Uniprot | |
R455 | Methylation | Uniprot | |
R459 | Methylation | Uniprot | |
S460 | Phosphorylation | Uniprot |
翻译修饰 - Q9BUB5 作为激酶
Substrate | Site | Source |
---|---|---|
O43597 (SPRY2) | S112 | Uniprot |
O43597 (SPRY2) | S121 | Uniprot |
P06730 (EIF4E) | S209 | Uniprot |
P09651 (HNRNPA1) | S192 | Uniprot |
P09651 (HNRNPA1) | S362 | Uniprot |
P09651 (HNRNPA1) | S363 | Uniprot |
P09651 (HNRNPA1) | S364 | Uniprot |
P23246 (SFPQ) | S8 | Uniprot |
P23246 (SFPQ) | S283 | Uniprot |
P47712 (PLA2G4A) | S727 | Uniprot |
Q9NP80 (PNPLA8) | S511 | Uniprot |
研究背景
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Cytoplasm.
Cytoplasm. Nucleus.
Ubiquitous.
Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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