Clock Antibody - #AF0323

Fig 4. Bioinformatics analysis of the gene pathways that were enriched in the N.g MDP–treated osteocytes and verification in vitro. (A and B) Pathway enrichment analysis showed that the differentially expressed genes, including clock and Rora, were associated with Circadian rhythm pathways. The size of the nodes and the intensity of the color indicate the gene number and mean P value. (C) qRT-PCR showed decreased expression of clock mRNA, n = 3. (D) Western blots showed decreased expression of CLOCK, RUNX2, and BGLAP. (E) Quantitative analysis of the expression of CLOCK protein. n = 3. (F) qRT-PCR showed increased expression of clock mRNA after osteogenic induction. (G) In vivo, immunohistochemistry showed increased CLOCK expression in bone mesenchymal stem cells (BMSCs) and osteoblasts, but decreased expression in osteocytes. Scale bar = 10 μm. (H and I) Quantitative analysis of immunohistochemical score of (G). Green arrow, BMSCs; blue arrow, osteoblast; red arrow, osteocyte; Control, the sample was treated with culture medium in vitro or saline only in vivo; MDP, the sample was treated with 1 μg/ml N.g MDP for 36 h in vitro or 2 μg N.g MDP dissolved in 100 μl of saline solution for 10 days in vivo.