TGF beta 1 Antibody - #AF1027

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产品: TGF beta 1 抗体
货号: AF1027
描述: Rabbit polyclonal antibody to TGF beta 1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: P01137
RRID: AB_2835389

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TGF beta1 Antibody detects endogenous levels of total TGF beta1.
RRID:
AB_2835389
引用格式: Affinity Biosciences Cat# AF1027, RRID:AB_2835389.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cartilage-inducing factor; CED; Differentiation inhibiting factor; DPD1; LAP; Latency-associated peptide; Prepro transforming growth factor beta 1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1; Transforming Growth Factor-ß1;

抗原和靶标

免疫原:

A synthesized peptide derived from human TGF beta1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
TGFB 1(7040)
描述:
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Multi-drug loaded electrospinning nanofibers promote healing with less scar formation by remodeling wound microenvironment via modulating TGF-β1/Smads/EN1 signal. Chemical Engineering Journal, 2024 [IF=13.3]
2). Phenytoin silver: a new nanocompound for promoting dermal wound healing via comprehensive pharmacological action. Theranostics, 2017 (PubMed: 28255340) [IF=12.4]

Application: WB    Species: human    Sample:

Figure 6. PnAg regulates gp130/Jak/Stat3 signaling pathway (A) and (B) NIH-3T3 and HaCat Cells were treated with PnAg at different concentrations and cell viability was tested using MTT analysis. (C) Wound healing assay reflected the effect of PnAg on cell migration. (D) Binding mode of PnAg in the active pocket of gp130. (E) and (F) MMPs activity and expression levels of Stat3, VEGF, TGFB-1, and TGFB1 detected using zymographic and Western blot assays. (G) Diagram of the proposed function of PnAg in wound inflammation and re-epithelialization controls.

Application: IHC    Species: rat    Sample:

Figure 2. PnAg promotes wound healing in SD rats. (A) Photographs of rat skin full-thickness excision wounds on different post-excision days. (B) Change in wound areas of SD rats after treatment; (C) and (D) Expression levels of collagen I, NF-κB, TGF-ß, MMP-2, and MMP-9 in tissues on day 7 and 17 detected by immunohistochemistry. (E) Histogram of protein expression levels in these tissues. (F) and (G) Histomorphological changes in wound tissues stained by Masson trichrome and HE on day 17.
3). 3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing. Journal of controlled release : official journal of the Controlled Release Society, 2025 (PubMed: 39955036) [IF=10.5]
4). An in situ tissue engineering scaffold with growth factors combining angiogenesis and osteoimmunomodulatory functions for advanced periodontal bone regeneration. JOURNAL OF NANOBIOTECHNOLOGY, 2021 (PubMed: 34404409) [IF=10.2]
5). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: IHC    Species: Mice    Sample: skin

Fig. 5 Histological analysis of skin defects in mice. A H&E staining in the three groups on days 7 and 14. The dotted lines indicate the size of the wound. B Masson staining in three groups on days 7 and 14. The dotted lines indicate the size of the wound. C Immunofluorescence staining of TGF-β1 in each group of skin sections on days 7 and 14. DAPI is stained blue, and TGF-β1 is stained green. D Immunofluorescence staining of CD31 in each group of skin sections on days 7 and 14. DAPI is stained blue, and CD31 is stained red. Arrows indicate the location of neovascularization. E, F Mean fluorescence intensity of TGF-β1 in each group of skin sections on days 7 and 14. G, H Mean fluorescence intensity of CD31 in each group of skin sections on days 7 and 14 (*p < 0.05, **p < 0.01, *** p < 0.001, n = 3)
6). LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease. EMBO molecular medicine, 2023 (PubMed: 36629048) [IF=9.0]

Application: WB    Species: Human    Sample: HK2 cells

Figure 4. LONP1 attenuated TGF‐β1‐induced mitochondrial dysfunction and fibrotic response in HK2 cells. AQuantification of mitochondrial ROS production (n = 4, biological replicates). B, CMeasurement of oxygen consumption rate (OCR) using an XF96 Extracellular Flux Analyzer. OSR, spare respiratory capacity (n = 8–10 in each group, biological replicates). DqRT‐PCR analysis of FN1, Collagen I, Collagen III, Collagen IV, α‐SMA and Vimentin (n = 3, biological replicates). E, FWestern blot and densitometric analysis for the expression of Collagen I, α‐SMA, and Vimentin (n = 3, two independent experiments were carried out). GQuantification of mitochondrial ROS production (n = 4, biological replicates). H, IMeasurement of OCR and OSR using an XF96 Extracellular Flux Analyzer (n = 8 or 10 in each group, biological replicates). JqRT‐PCR analysis of FN1, Collagen I, Collagen III, Collagen IV, α‐SMA and Vimentin (n = 3, biological replicates). K, LWestern blot and densitometric analysis for the expression of Collagen I, α‐SMA, and Vimentin (n = 3, two independent experiments were carried out). Data information: In (A–F), HK2 cells were transfected with vector or Lonp1 plasmid, and then treated with TGF‐β1 (10 ng/ml) for 24 h. In (G–L), HK2 cells were transfected with vector or Lonp1 shRNA, and then treated with TGF‐β1 (10 ng/ml) for 24 h. Data are presented as mean ± SEM. One‐way ANOVA.
7). Application of In Situ Mucoadhesive Hydrogel with Anti-Inflammatory and Pro-Repairing Dual Properties for the Treatment of Chemotherapy-Induced Oral Mucositis. ACS applied materials & interfaces, 2024 (PubMed: 38970482) [IF=8.3]
8). Multifunctional human serum albumin fusion protein as a docetaxel nanocarrier for chemo-photothermal synergetic therapy of ovarian cancer. ACS Applied Materials & Interfaces, 2022 (PubMed: 35441508) [IF=8.3]
9). DNMT3a Deficiency Contributes to Anesthesia/Surgery-Induced Synaptic Dysfunction and Cognitive Impairment in Aged Mice. Aging cell, 2024 (PubMed: 39722450) [IF=8.0]
10). Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages. International Journal of Biological Macromolecules, 2023 (PubMed: 37364804) [IF=7.7]
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