Caspase 8 Antibody - #AF6442

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产品: Caspase 8 抗体
货号: AF6442
描述: Rabbit polyclonal antibody to Caspase 8
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q14790
RRID: AB_2835266

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Caspase 8 Antibody detects endogenous levels of total Caspase 8.
RRID:
AB_2835266
引用格式: Affinity Biosciences Cat# AF6442, RRID:AB_2835266.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptotic cysteine protease; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP4; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase-8 subunit p10; CED 3; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MCH5; MGC78473; MORT1 associated ced 3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582;

抗原和靶标

免疫原:

A synthesized peptide derived from human Caspase 8, corresponding to a region within the internal amino acids.

基因/基因ID:
CASP8(841)
描述:
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). Gut microbes exacerbate systemic inflammation and behavior disorders in neurologic disease CADASIL. Microbiome, 2023 (PubMed: 37684694) [IF=13.8]
2). Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy. International journal of biological sciences, 2024 (PubMed: 38725851) [IF=8.2]
3). Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance. Cell Death & Disease, 2018 (PubMed: 29549251) [IF=8.1]

Application: IHC    Species: human    Sample: ascites cells

Fig. 5 |Apoptosis is increased in the EOC chemosensitive group. a, b IHC and IF analysis of apoptotic protein expression in ovarian cancer samples. c, d Western blot analysis of apoptotic proteins expressed in the no-chemotherapy group and chemosensitive group (***p<0.001,**p<0.01, *p<0.05). e qRT-PCR analysis of the average relative mRNA expression levels of apoptosis-related genes in the no-chemotherapy group and chemosensitive group (*p<0.05)

Application: IF/ICC    Species: human    Sample: ascites cells

Fig. 5 |Apoptosis is increased in the EOC chemosensitive group. a, b IHC and IF analysis of apoptotic protein expression in ovarian cancer samples. c, d Western blot analysis of apoptotic proteins expressed in the no-chemotherapy group and chemosensitive group (***p<0.001,**p<0.01, *p<0.05). e qRT-PCR analysis of the average relative mRNA expression levels of apoptosis-related genes in the no-chemotherapy group and chemosensitive group (*p<0.05)

Application: WB    Species: human    Sample: ascites cells

Fig. 5 |Apoptosis is increased in the EOC chemosensitive group. a, b IHC and IF analysis of apoptotic protein expression in ovarian cancer samples. c, d Western blot analysis of apoptotic proteins expressed in the no-chemotherapy group and chemosensitive group (***p<0.001,**p<0.01, *p<0.05). e qRT-PCR analysis of the average relative mRNA expression levels of apoptosis-related genes in the no-chemotherapy group and chemosensitive group (*p<0.05)
4). A novel trans-acting lncRNA of ACTG1 that induces the remodeling of ovarian follicles. International journal of biological macromolecules, 2023 (PubMed: 37276900) [IF=7.7]
5). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.2]

Application: WB    Species: mouse    Sample: testis

Fig. 7. |SiNPs affected the protein expressions of ATM/p53 and TNF-α/TNFR I-mediated signaling pathways. (A) The protein expressions of ATM, p53, Bcl-2, Bax,TNF-α, TNFR I, Caspase-8, and Caspase-3 were detected in the testis of control group and SiNPs group.
6). Discovery of novel peptide-dehydroepiandrosterone hybrids inducing endoplasmic reticulum stress with effective in vitro and in vivo anti-melanoma activities. European journal of medicinal chemistry, 2024 (PubMed: 38467086) [IF=6.0]
7). SOX11 and FAK participate in the stretch‑induced mechanical injury to alveolar type 2 epithelial cells. International Journal of Molecular Medicine, 2021 (PubMed: 33236128) [IF=5.7]

Application: WB    Species: Human    Sample: AT2 cells

Figure 6 (A-C) Western blot analysis showing caspase-3/8 expression in AT2 cells. The overexpression of SOX11 inhibited, whilst the knockdown of SOX11 increased caspase-3/8 expression. Furthermore, FAK antagonism blocked the effect of SOX11 overexpression on caspase-3/8 expression. Data are presented as the means ± standard error of the mean. *P<0.05, **P<0.01. AT2, alveolar type II; SOX, Sex-determining gene on the Y chromosome related high mobility group box; FAK, focal adhesion kinase.
8). Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. International journal of molecular sciences, 2024 (PubMed: 38473715) [IF=5.6]

Application: WB    Species: Mouse    Sample: GCs

Figure 2. SLCO3A1 promotes the cell cycle-related processes but inhibits the apoptosis of GCs. The cell cycle distribution was analyzed by flow cytometric transfected with OE-SLCO3A1 (A) or KD-SLCO3A1 (B). Green: cells in the G0-G1 phase, Yellow: cells in S phase, Blue: cells in G2-M phase. (C) RT-qPCR analysis of cycle-related genes expression transfected with OE-NC or OE-SLCO3A1. (D) Western blot analysis of CCNE1, CDK2 and CCND1 transfected with OE-NC or OE-SLCO3A1. (E) RT-qPCR analysis of cycle-related genes expression transfected with KD-NC or KD-SLCO3A1. (F) Western blot analysis of CCNE1, CDK2 and CCND1 transfected with KD-NC or KD-SLCO3A1. Flow cytometry assay of the apoptosis rate transfected with OE-SLCO3A1 (G) or KD-SLCO3A1 (H). Q1: dead cells and cell debris, Q2: late apoptotic cells, Q3: early apoptotic cells, Q4: negative cells. RT-qPCR analysis of pro-apoptosis related genes expression transfected with OE-SLCO3A1 (I) or KD-SLCO3A1 (K). Western blot analysis of CASP3 and CASP8 transfected with OE-SLCO3A1 (J) or KD-SLCO3A1 (L). All of the above experimental results were obtained in the COV434 cells. * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05.
9). Akkermansia muciniphila Aspartic Protease Amuc_1434* Inhibits Human Colorectal Cancer LS174T Cell Viability via TRAIL-Mediated Apoptosis Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020 (PubMed: 32403433) [IF=5.6]

Application: WB    Species: human    Sample: LS174T cells

Figure 5. | Amuc_1434* mediated the activation of the apoptosis pathway in LS174T cells. (A) The expression of death receptor 4 (DR4), death receptor 5 (DR5), cysteinyl aspartate specific proteinase 8(caspase 8) and cysteinyl aspartate specific proteinase 3 (caspase 3) induced by Amuc_1434* in LS174T cells was dependent on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). (a) LS174T cells were treated with 8 and 64 µg/mL Amuc_1434* for 24 h, respectively. The cell lysates were analyzed by Western blot.
10). Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis. LIFE SCIENCES, 2020 (PubMed: 32702445) [IF=5.2]

Application: WB    Species: rat    Sample: liver

Fig. 7. |Naringin abrogates mitochondrial apoptosis. (H–J) caspase 8, caspase 9, and caspase 3 were determined by immunoblotting in liver tissue. Mean ± S.E.M (n = 3), *Control vs NASH; #NASH vs NAR. *P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001.
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