AMPK alpha Antibody | Affinity Biosciences LTD|亲科生物官网

$Fig. 5. Effects of stigmasterol treatment on inflammatory pathways in Aβ42 oligomers induced BV2 cells when treated with AMPK inhibitor. Cells were pretreated with Compound C (10 μM) for 4 h, and then treated with Aβ42 oligomers (1 μM) for 24 h, followed by treatment with stigmasterol (20 μM) for 4 h. (A,B) Representative western blot analysis of AMPK signaling. GAPDH immunoreactivity was used as a loading control. (C-E) Representative western blot analysis of NF-κB signaling. The cytosolic and nuclear fractions were prepared and analyzed with total NF-κB p65. α-Tubulin immunoreactivity was used as a loading control in the cytosolic fraction, Histone H3 was used as the loading control in the nuclear fraction. (F-H) Representative western blot analysis of NLRP3 signaling, including NLRP3 and Caspase-1, p20. GAPDH immunoreactivity was used as a loading control (I, J) Concentration of TNFα and IL-1β. Data were presented as the mean ± SEM from three independent experiments. One-way ANOVA with Tukey’s multiple comparison test revealed a difference between groups.$

AMPK alpha Antibody - Fig.

AMPK alpha Antibody - Figure 3 Effect of AICAR treatment on the activities of AMPK and Drp1 (A,B) Representative immunofluorescence staining images of the expression of AMPK and Drp1 in the spinal dorsal horn of sham and CIBP rats.

AMPK alpha Antibody - Figure 2 Relative proteins variation in C6 cells administration of JWH133 (5 μM) for 48 h.

AMPK alpha Antibody - Fig.

AMPK alpha Antibody - Figure 5 Effects of metformin on the AMPK/ERK signaling pathway in ox-LDL-treated macrophages.

AMPK alpha Antibody - Fig.

AMPK alpha Antibody - Figure 6.

AMPK alpha Antibody - Figure 4 Effects of AdipoRon on p65 and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation under proinflammatory conditions.

$Figure 5 p53/AMPK/mTOR pathway was required for S100P-mediated autophagy regulating chemosensitivity. A. p53 expression levels were detected in S100P-deficient cells and S100P-overexpression cells by western blot analysis respectively; B. The expressions of nuclear/cytosolic p53 in S100P-deficient cells and S100P-overexpression cells were assayed by western blot. Fibrillarin was a nuclear fraction control and tubulin was a cytoplasmic fraction control. C-p53, cytosolic p53; N-p53, nuclear p53; C. HL-60 and Jurkat cells were transfected with S100P shRNA or control shRNA and then pre-treated with Tenovin-6 (5 μM) for 8 h. Cells were subsequently treated for 2 h with HBSS, and then LC3-I/II, p62, p53, p-AMPK, AMPK, p-mTOR, mTOR, and S100P levels were assayed by western blot (n=3, *P0.05 vs. shS100P group); D. HL-60 and Jurkat cells were transfected with S100P shRNA or control shRNA and then pre-treated with Compound C (20 μM) for 6 h. Cells were subsequently treated for 2 h with HBSS, and then LC3-I/II, p62, p53, p-AMPK, AMPK, p-mTOR and mTOR levels were assayed by western blot. CC, Compound C (n=3, *P0.05 vs. shS100P group); E. HL-60 and Jurkat cells were transfected with S100P shRNA or control shRNA and then pre-treated with Tenovin-6 (5 μM) for 8 h. Cells were subsequently treated for 24 h with ADM (0.2 μg/ml) or 48 h with Ara-C (4 μM), then cell viability was assayed using a CCK-8 kit (n=3, *P$

AMPK alpha Antibody - Figure 5 p53/AMPK/mTOR pathway was required for S100P-mediated autophagy regulating chemosensitivity.

AMPK alpha Antibody - Fig.

AMPK alpha Antibody - Figure 6 Effect of WLS on regulating the AMPK/mTOR/ULK1 signaling pathway in MAFLD rats.

AMPK alpha Antibody - Fig.

AMPK alpha Antibody - Figure 7 PI3K/Akt pathway inhibition and AMPK pathway activation are involved in the schisandrin B-induced increase in KCP expression in high glucose-treated HK2 cells.

AMPK alpha Antibody - Figure 6 Effects of PLB on the AMPK pathway in the lung tissues of nonobese and obese asthmatic mice.

AMPK alpha Antibody - Figure 3.

AMPK alpha Antibody - Fig.

AMPK alpha Antibody - Figure 3.

产品:	AMPK alpha 抗体
货号:	AF6423
描述:	Rabbit polyclonal antibody to AMPK alpha
应用:	WB IHC IF/ICC
文献验证:	WB, IHC, IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号:	Q13131 \| P54646
RRID:	AB_2835253

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human, Mouse, Rat

克隆:

Polyclonal

特异性:

AMPK alpha Antibody detects endogenous levels of total AMPK alpha.

RRID:

AB_2835253
引用格式: Affinity Biosciences Cat# AF6423, RRID:AB_2835253.

偶联:

Unconjugated. 130

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AAPK1_HUMAN; ACACA kinase; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1; AMPK alpha 1 chain; AMPK; AMPK subunit alpha-1; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; HMGCR kinase; hormone sensitive lipase kinase; Hydroxymethylglutaryl CoA reductase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; OTTHUMP00000161795; OTTHUMP00000161796; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; SNF1A; Tau protein kinase PRKAA1; wu:fa94c10; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AAPK2_HUMAN; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPKalpha2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; PRKAA; PRKAA2; Protein kinase AMP activated alpha 2 catalytic subunit; Protein kinase AMP activated catalytic subunit alpha 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human AMPK alpha, corresponding to a region within the internal amino acids.

基因/基因ID:

PRKAA1(5562) | PRKAA2(5563)

描述:

AMPKA2 a protein kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma).

文献引用

1). Enhanced Antiglioma Effect by a Vitamin D3-Inserted Lipid Hybrid Neutrophil Membrane Biomimetic Multimodal Nanoplatform. ACS nano, 2024 (PubMed: 39696957) [IF=17.1]

2). Targeting Histone Deacetylase 11 with a Highly Selective Inhibitor for the Treatment of MASLD. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39976110) [IF=15.1]

3). Injectable Hierarchical Bioactive Hydrogels with Fibroblast Growth Factor 21/Edaravone/Caffeic Acid Asynchronous Delivery for Treating Parkinson's Disease. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39630931) [IF=15.1]

4). Autophagy-enhanced nanosonosensitizer mediated sonodynamic therapy for post-myocardial infarction neuromodulation and arrhythmia prevention. Theranostics, 2025 (PubMed: 39990226) [IF=12.4]

5). Regulatory effects mediated by ulvan oligosaccharide and its zinc complex on lipid metabolism in high-fat diet-fed mice. Carbohydrate Polymers, 2023 (PubMed: 36372481) [IF=10.7]

6). NCAPD2 inhibits autophagy by regulating Ca2+/CAMKK2/AMPK/mTORC1 pathway and PARP-1/SIRT1 axis to promote colorectal cancer. CANCER LETTERS, 2021 (PubMed: 34229059) [IF=9.1]

Application: WB Species: Human Sample: CRC cells

Fig. 2. NCAPD2 inhibited cell autophagy and disrupted autophagic flux via Ca2+/CAMKK2/AMPK/mTORC1 pathway. (A) Western blot analyses for phosphorylated mTOR (p-mTOR, S2448), phosphorylated p70S6K (p-p70S6K, T389/412), phosphorylated 4E-BP1 (p-4E-BP1, T70) and phosphorylated AKT (p-AKT, S473) in CRCC cells with different treatments as indicated. (B) Western blot of indicated proteins in cells treated with mTORC1 inhibitor Rapamycin (3 nM, 24h). (C) Immunofluorescence staining of LC3II (red) and P62 (red) in CRC cells with different treatments as indicated. Merged images represented overlays of LC3II or P62 and nuclear staining by DAPI (blue). (D) Intracellular Ca2+ levels were analyzed by flow cytometry after staining with the fluorescent probe Fluo-3, AM in CRC cells. (E) Representative Western blot gel documents of phosphorylated CAMKK2(S511), phosphorylated AMPK(T172), phosphorylated mTOR(S2448), Beclin, ATG5, P62, LC3II in CRC cells with different treatments. (F) Western blots of indicated proteins in cells treated with an inhibitor of microsomal Ca2+-ATPase Thapsigargin (1 μM, 6h) and Ca2+ chelator BAPTA-AM (10 μM, 12h) respectively. Results are shown as mean ± s.d, *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t-test. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

7). Amelioration action of gastrodigenin rhamno-pyranoside from Moringa seeds on non-alcoholic fatty liver disease. Food Chemistry, 2022 (PubMed: 35086000) [IF=8.5]

8). The immunomodulatory role of irisin on osteogenesis via AMPK-mediated macrophage polarization. International Journal of Biological Macromolecules, 2020 (PubMed: 31843619) [IF=7.7]

Application: WB Species: Mouse Sample: Raw264.7 cells

Fig. 8. Knockdown of AMPK-α impaired the effect of irisin on M2 macrophage polarization and compromised osteogenic ability in direct co-culture system. Raw264.7 cells were transfected with AMPK-α siRNA or scramble siRNA, respectively. (A) Knockdown efficiency was confirmed by western blot. GAPDH was used to verify equivalent loading. (B) AMPK-α siRNA abrogated irisin-induced phosphorylation of AMPK-α. (C) Knockdown of AMPK-α caused a decrement of CD206-APC expression in irisin-treated macrophages. (D-F) Knockdown of AMPK-α reversed the inductive effect of irisin on the expression of ARG-1 and TGF-β1, but increased TNF-α expression. (G-H) Knockout of AMPK-α reduced the osteogenic ability of irisin-treated macrophage. The data are represented as the mean ± SD from three independent experiments. # p < 0.05, ##p < 0.01 compared with si NC group. Unstained, macrophages without treatment and antibody incubation; w, week.

Application: WB Species: Sample: M0 and M1 macrophages

Fig 7.| Irisin induced phosphorylation of AMPK-α in M0 and M1 macrophages. (A)Irisin induced phosphorylation of AMPK-α, when compared to control medium, LPS plus IFN-γ, and IL-4 groups

9). A postbiotic exopolysaccharide synergizes with Lactobacillus acidophilus to reduce intestinal inflammation in a mouse model of colitis. International journal of biological macromolecules, 2025 (PubMed: 39732236) [IF=7.7]

10). Exosomes derived from miR-26a-modified MSCs promote axonal regeneration via the PTEN/AKT/mTOR pathway following spinal cord injury. Stem Cell Research & Therapy, 2021 (PubMed: 33820561) [IF=7.5]

Application: WB Species: rat Sample: PC14 cells

FIGURE S2 | miR-26a-overexpressing exosomes inhibited autophagic activity and promoted axonal generation in PC12 cells. (a) The ability of Exos-26a to generate neurofilament (red fluorescent dye) in PC12 cells, which could be reversed by rapamycin. (b, c) Representative images of western blots used to determine the expression levels of NF, mTOR, p-mTOR, AMPK, p-AMPK, S6K, p-S6K, ULK1, p-ULK1, and p62 and semiquantification of the data. RAP indicates miR-26a exosome and rapamycin (100 nM) treatment for 48 h before lysis. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group by t test or ANOVA. #P < 0.05 and ##P < 0.01 compared with the RAP group by t test. n = 3 for each group.

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AMPK alpha Antibody - #AF6423

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MSDS

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WB Handbook

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产品描述

抗原和靶标

文献引用

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