AMPK alpha 1 Antibody - #AF6422

>>买2送1 已结束>>
>>赠JD卡 已结束>>
(3)   (8)  
产品: AMPK alpha 1 抗体
货号: AF6422
描述: Rabbit polyclonal antibody to AMPK alpha 1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q13131
RRID: AB_2835252

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
AMPK alpha 1 Antibody detects endogenous levels of total AMPK alpha 1.
RRID:
AB_2835252
引用格式: Affinity Biosciences Cat# AF6422, RRID:AB_2835252.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AAPK1_HUMAN; ACACA kinase; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1; AMPK alpha 1 chain; AMPK; AMPK subunit alpha-1; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; HMGCR kinase; hormone sensitive lipase kinase; Hydroxymethylglutaryl CoA reductase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; OTTHUMP00000161795; OTTHUMP00000161796; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; SNF1A; Tau protein kinase PRKAA1; wu:fa94c10;

抗原和靶标

免疫原:

A synthesized peptide derived from human AMPK alpha 1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PRKAA1(5562)
描述:
AMPKA1 a protein kinase of the CAMKL family that plays a central role in regulating cellular and organismal energy balance in response to the balance between AMP/ATP, and intracellular Ca(2+) levels.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2019 (PubMed: 31173178) [IF=5.7]

Application: WB    Species: mouse    Sample: MC3T3‑E1 cells

Figure 6.| AMPK inhibition suppresses the effects of Sr. The MC3T3‑E1 cells were pre‑incubated with 5 µM compound C prior to osteogenic induction. (A‑E) Western blot analysis results for AMPK, p‑AMPK, LC‑3 I/II and OCN. Tubulin was used as an internal control. Quantitative analysis of AMPK, p‑AMPK, LC‑3 I/II and OCN is also shown, expressed as the means ± SD (n=3 for each group). **P<0.01; ***P<0.005; and ****P<0.001 compared with the control group. Sr, strontium chloride; AMPK, AMP‑activated protein kinase; LC, microtubule‑associated protein 1 light chain 3; OCN, osteocalcin.
2). Profile analysis and functional modeling identify circular RNAs in nonalcoholic fatty liver disease as regulators of hepatic lipid metabolism. Frontiers in genetics, 2022 (PubMed: 36186461) [IF=3.7]

Application: IHC    Species: mouse    Sample: hepatocytes

FIGURE 6. miR-466i-3p and miR-669c-3p activation induces AMPK-α1 downregulation and expressive promotion of lipogenic genes. (A,B) Both miR-466i-3p (A) and miR-669c-3p (B) targeted AMPK-α1 mRNA by the complementation of ‘seed sequence’ (miRNA) and 3′ untranslated region (mRNA). Triangles and circles labeling AMPK-α1 mRNA with serial numbers reflect the complementation sites of miR-466i-3p and miR-669c-3p, respectively. (C–E) miR-466i-3p and miR-669c-3p activation in the NAFLD group led to mRNA decrease in AMPK-α1 (C), whereas transcriptional increase in SREBP1 (D) and FASN (E). (F–I) Western blotting (F) semi-quantitatively confirmed the downregulation of AMPK-α1 (G) and upregulation of both SREBP1 (H) and FASN (I) in the NAFLD group. (J) Immunohistochemical staining revealed the expressive alterations of AMPK-α1, SREBP1, and FASN in hepatocytes. The data was presented as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Application: WB    Species: mouse    Sample: hepatocytes

FIGURE 6. miR-466i-3p and miR-669c-3p activation induces AMPK-α1 downregulation and expressive promotion of lipogenic genes. (A,B) Both miR-466i-3p (A) and miR-669c-3p (B) targeted AMPK-α1 mRNA by the complementation of ‘seed sequence’ (miRNA) and 3′ untranslated region (mRNA). Triangles and circles labeling AMPK-α1 mRNA with serial numbers reflect the complementation sites of miR-466i-3p and miR-669c-3p, respectively. (C–E) miR-466i-3p and miR-669c-3p activation in the NAFLD group led to mRNA decrease in AMPK-α1 (C), whereas transcriptional increase in SREBP1 (D) and FASN (E). (F–I) Western blotting (F) semi-quantitatively confirmed the downregulation of AMPK-α1 (G) and upregulation of both SREBP1 (H) and FASN (I) in the NAFLD group. (J) Immunohistochemical staining revealed the expressive alterations of AMPK-α1, SREBP1, and FASN in hepatocytes. The data was presented as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
3). Liposomal Silybin Improves Glucose and Lipid Metabolisms in Type 2 Diabetes Mellitus Complicated with Non-Alcoholic Fatty Liver Disease via AMPK/TGF-β1/Smad Signaling. The Tohoku journal of experimental medicine, 2023 (PubMed: 37344419) [IF=1.7]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.