SREBP1 Antibody - #AF6283

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产品: SREBP1 抗体
货号: AF6283
描述: Rabbit polyclonal antibody to SREBP1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat, Pig, Sheep
预测: Pig, Horse, Sheep, Dog
蛋白号: P36956
RRID: AB_2835134

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Pig, Sheep
克隆:
Polyclonal
特异性:
SREBP1 Antibody detects endogenous levels of total SREBP1.
RRID:
AB_2835134
引用格式: Affinity Biosciences Cat# AF6283, RRID:AB_2835134.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human SREBP1, corresponding to a region within the internal amino acids.

基因/基因ID:
SREBF1(6720)
描述:
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

文献引用

1). Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. Cell metabolism, 2023 (PubMed: 33186558) [IF=27.7]
2). Targeting Histone Deacetylase 11 with a Highly Selective Inhibitor for the Treatment of MASLD. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39976110) [IF=15.1]

Application: WB    Species: human    Sample: HSP90 of HepG2 cells

Figure 5 Effects of B6 on de novo lipogenesis and fatty acid oxidation in AML12 cells. A,B) Oil Red O staining and optical density of AML12 cells treated with B6 of different concentration for 24 h. The experiment was conducted independently on four occasions. C) Protein expression of HDAC11, SREBP1c, FASN, SCD1, and HSP90 of HepG2 cells treated with FFA and 0.5 × 10−6 m B6 for 24 h (n = 3 biological replicates). D–F) Relative normalized mRNA expression of SREBP1c, FASN, and SCD1 of indicated cells (n = 4 biological replicates). mRNA level of β-actin was used as normalized control. G) Mito-Tracker Deep Red FM staining of indicated cells. The experiment was conducted independently on three occasions. H) Mitochondrial oxygen consumption rate in indicated cells (n = 3 biological replicates). I) Protein expression of CPT1A, PGC1α, PPARα, and HSP90 in indicated cells (n = 3 biological replicates). J–L) Relative normalized mRNA expression of CPT1A, PGC1α, and PPARα of indicated cells (n = 4 biological replicates). mRNA level of β-actin was used as normalized control. Data are shown as mean ± SD. The p-values were calculated by one-way ANOVAs.
3). Melatonin receptor 1a alleviates sleep fragmentation-aggravated testicular injury in T2DM by suppression of TAB1/TAK1 complex through FGFR1. Acta pharmaceutica Sinica. B, 2025 (PubMed: 40698135) [IF=14.7]
4). CCDC92 deficiency ameliorates podocyte lipotoxicity in diabetic kidney disease. Metabolism: clinical and experimental, 2024 (PubMed: 37952690) [IF=10.8]
5). Overexpression of NAG-1/GDF15 prevents hepatic steatosis through inhibiting oxidative stress-mediated dsDNA release and AIM2 inflammasome activation. Redox Biology, 2022 (PubMed: 35504134) [IF=10.7]
6). Nontargeted metabolomics combining with intestinal microbiota revealed the potential mechanisms of DHA/EPA - acetylated astaxanthin esters in regulating the abnormal lipid metabolism. FOOD RESEARCH INTERNATIONAL, 2025 [IF=8.0]

Application: WB    Species: Mouse    Sample:

Fig. 3. DA and EA supplementation ameliorated β-oxidation and fatty acid production in HFD mice. (A) Key protein expressions of β-oxidation in the liver. (Bsingle bondC) Quantitative analysis of the CPT1A and PPAR-α. (D) Key protein expressions of fatty acid production in the liver. (E-F) Quantitative analysis of the SREBP-1 and FAS.
7). Anti-b diminishes hyperlipidaemia and hepatic steatosis in hamsters and mice by suppressing the mTOR/PPARγ and mTOR/SREBP1 signalling pathways. British journal of pharmacology, 2024 (PubMed: 39614407) [IF=7.3]
8). MED15 is upregulated by HIF-2α and promotes proliferation and metastasis in clear cell renal cell carcinoma via activation of SREBP-dependent fatty acid synthesis. Cell death discovery, 2024 (PubMed: 38649345) [IF=7.0]
9). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022 [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.
10). β-patchoulene improves lipid metabolism to alleviate non-alcoholic fatty liver disease via activating AMPK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2021 (PubMed: 33341045) [IF=6.9]

Application: WB    Species: Human    Sample: L02 cell

Fig. 4. β-PAE inhibits the expression of hepatic lipid synthesis-related proteins and genes in HFD-fed rats. (A–F) Western blot analysis on the expression of proteins referred to hepatic lipid synthesis including SREBP-1c, ACC1, p-ACC1, FASN, SCD1 and HMG-CR; (G–H) The mRNA expression of SREBP-1c and HMG-CR. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.
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