ELK1 Antibody - #AF6212

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产品: ELK1 抗体
货号: AF6212
描述: Rabbit polyclonal antibody to ELK1
应用: WB IHC IF/ICC IP
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: P19419
RRID: AB_2835093

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ELK1 Antibody detects endogenous levels of total ELK1.
RRID:
AB_2835093
引用格式: Affinity Biosciences Cat# AF6212, RRID:AB_2835093.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ELK 1; Elk1; ELK1 member of ETS oncogene family; ELK1 protein; ELK1, ETS transcription factor; ELK1_HUMAN; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS like gene 1; Member of ETS oncogene family; Oncogene Elk1; Tyrosine kinase (ELK1) oncogene;

抗原和靶标

免疫原:

A synthesized peptide derived from human ELK1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
ELK1(2002)
描述:
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). Dissecting the process of human neutrophil lineage determination by using alpha-lipoic acid inducing neutrophil deficiency model. Redox biology, 2022 (PubMed: 35797799) [IF=10.7]
2). Small-molecule α-lipoic acid targets ELK1 to balance human neutrophil and erythrocyte differentiation. Stem cell research & therapy, 2024 (PubMed: 38589882) [IF=7.5]
3). Protective effect of remdesivir against pulmonary fibrosis in mice. Frontiers in Pharmacology, 2021 (PubMed: 34512328) [IF=5.6]

Application: WB    Species: Mice    Sample: lung tissues

FIGURE 10 Remdesivir suppress BLM-induced pulmonary fibrosis in mice via inhibiting TGF-β1-Smad and non-Smad signaling pathway in vivo. Protein levels of p-Smad2, Smad2, p-Smad3, Smad3, p-P38, P38, p-JNK, JNK, p-ERK, ERK, p-AKT, and AKT were verified by Western blot in lung tissues. GAPDH was used as an internal reference in densitometric analysis. Data was presented as the means ± SD, n = 3. ** p < 0.01, *** p < 0.001.
4). LncRNA 122049 suppresses apoptosis of renal tubular epithelial cells in ischemic AKI by targeting the miR‐330‐5p/ELK1 axis. The FASEB Journal, 2022 (PubMed: 35695811) [IF=4.4]

Application: WB    Species: Mice    Sample: BUMPT cells

FIGURE 6ELK1 was identified as a target gene of miR-330-5p and mediated the renal cell apoptosis. BUMPT cells were transfected with 100 nM miR-330-5p mimics or ELK1 siRNA or scramble and then treated with or without 2 h ischemia and 2 h reperfusion. (A) Putative miR-330-5p complementary binding sites in the 3′ UTR of ELK1 mRNA. (B) The examination of luciferase activities after co-transfection with the 3′ UTR luciferase reporter vector of WT- or MUT-ELK1 and miR-330-5p mimic or miR-negative control (NC). (C and D) Real-time qPCR and western blot analysis of ELK1 and β-Tubulin. (E) The FCM detection of BUMPT cells apoptosis. (F) The representative of apoptosis rate. (G) Immunoblot analysis of cleaved caspase-3 and caspase-3. (H) Densitometric measurement of immunoblot bands. Data are expressed as mean ± SD (n = 6). #p < .05, versus scramble with saline group; *p < .05, versus scramble with I/R group or ELK1 3′ UTR WT plus miR-330-5p mimics versus other groups.
5). ANXA3 interference inactivates ERK/ELK1 pathway to mitigate inflammation and apoptosis in sepsis-associated acute lung injury. Molecular immunology, 2024 (PubMed: 38310670) [IF=3.2]

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