产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6200, RRID:AB_2835081.
展开/折叠
Activator protein 1; AP 1; AP1; Jun D; jun D proto oncogene; Jund; JunD FL isoform; JUND_HUMAN; Transcription factor jun D; Transcription factor jun-D;
抗原和靶标
- P17535 JUND_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSYAGGAGGAGGAATVAFAAEPVPFPPPPPPGALGPPRLAALKDEPQTVPDVPSFGESPPLSPIDMDTQERIKAERKRLRNRIAASKCRKRKLERISRLEEKVKTLKSQNTELASTASLLREQVAQLKQKVLSHVNSGCQLLPQHQVPAY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17535 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S27 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| K97 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | P45983 (MAPK8) , P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| T117 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| K127 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K240 | Sumoylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot |
研究背景
Transcription factor binding AP-1 sites.
Phosphorylated by MAP kinases MAPK8 and MAPK10; phosphorylation is inhibited in the presence of MEN1.
Nucleus.
Binds DNA as a dimer (By similarity). Interacts (via MBM motif) with MEN1; this interaction represses transcriptional activation. Interacts with MAPK10; this interaction is inhibited in the presence of MEN1.
Belongs to the bZIP family. Jun subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
限制条款
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