CD31 Antibody - #AF6191

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产品: CD31 抗体
货号: AF6191
描述: Rabbit polyclonal antibody to CD31
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Rat
预测: Mouse, Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P16284
RRID: AB_2835074

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Rat
克隆:
Polyclonal
特异性:
CD31 Antibody detects endogenous levels of total CD31.
RRID:
AB_2835074
引用格式: Affinity Biosciences Cat# AF6191, RRID:AB_2835074.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adhesion molecule; CD31; CD31 antigen; CD31 EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA; GPIIA'; PECA1; PECA1_HUMAN; Pecam 1; PECAM 1 CD31 EndoCAM; PECAM; PECAM-1; Pecam1; Platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human CD31, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PECAM1(5175)
描述:
PECAM-1 is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Microtubes with gradient decellularized porcine sciatic nerve matrix from microfluidics for sciatic nerve regeneration. Bioactive materials, 2022 (PubMed: 36185737) [IF=18.9]
2). A biomimetic piezoelectric scaffold with sustained Mg2+ release promotes neurogenic and angiogenic differentiation for enhanced bone regeneration. Bioactive materials, 2022 (PubMed: 37056250) [IF=18.9]

Application: IF/ICC    Species: Rat    Sample: bone tissue

Fig. 8. Histological, immunohistological, and immunofluorescence staining for the evaluation of bone regeneration. (A) H&E staining and Masson's trichrome staining for sections collected at 8 W post-operation. (B) Immunohistochemical staining of TRAP and OCN expression at 8 W post-operation. (C) Immunofluorescence staining of CD31 (red), CGRP (green), and DAPI (blue) expression at 4 W and 8 W post-operation.
3). Sea Cucumber-Inspired Microneedle Nerve Guidance Conduit for Synergistically Inhibiting Muscle Atrophy and Promoting Nerve Regeneration. ACS nano, 2024 (PubMed: 38776414) [IF=15.8]
4). Biomimetic Nanoparticle-Mediated Target Delivery of Hypoxia-Responsive Plasmid of Angiotensin-Converting Enzyme 2 to Reverse Hypoxic Pulmonary Hypertension. ACS Nano, 2023 (PubMed: 37071566) [IF=15.8]
5). Turmeric-Derived Nanoparticles Functionalized Aerogel Regulates Multicellular Networks to Promote Diabetic Wound Healing. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38441389) [IF=15.1]
6). Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis. Autophagy, 2022 (PubMed: 34872436) [IF=14.6]

Application: IF/ICC    Species: Mouse    Sample:

Figure 5. Activated PLA2G4E hindered the survival of random-pattern skin flaps. (A) Photograph of random-pattern skin flaps on mice treated with saline, AAV-scramble-I or AAV-Pla2g4e shRNA at different times (0, 1, 3 and 7 days after surgery). (B) Comparison of the survival area among the three groups on postoperative day 7. Data are expressed as the means ± SEM (n = 6). (C) LDBF of random-pattern skin flaps on mice at 1 and 7 days after surgery in each group. (D) Comparison of signal intensity of blood flow in random-pattern skin flaps among the three groups on postoperative day 7. Data are expressed as the means ± SEM (n = 6). (E) Immunofluorescence staining of PECAM1 and ACTA2 in the skin from area II in each group on postoperative day 7. Scale bars: 100 μm. (F) Comparison of the density of PECAM1-ACTA2-positive blood vessels among the three groups. Data are expressed as the means ± SEM (n = 6). (G) F-CHP staining for the detection of damaged collagen in the skin from area II in each group on postoperative day 7. Scale bars: 100 μm. (H) Comparison of the integrated intensity of F-CHP among the three groups. Data are expressed as the means ± SEM (n = 6). Significance: *p < 0.05.
7). Delivery of therapeutic miRNAs using nanoscale zeolitic imidazolate framework for accelerating vascularized bone regeneration. Chemical Engineering Journal, 2022 [IF=13.3]
8). A quaternized chitin derivatives, egg white protein and montmorillonite composite sponge with antibacterial and hemostatic effect for promoting wound healing. Composites Part B: Engineering, 2022 [IF=12.7]
9). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2024 (PubMed: 39248296) [IF=12.5]

Application: WB    Species: Rat    Sample:

Figure 2. POL promoted BMSCs-mediated angiogenesis. (A-B) The protein expression levels of VEGFA and CD31 were evaluated by western blot (n = 3); (C-D) Immunofluorescence staining of VEGFA and CD31 (scale bar = 200 μm); (E) Images of transwell migration assay (scale bar = 100 μm); (F) Quantification of transwell migration assay (n = 5); (G) Images of scratch wound assay (scale bar = 100 μm); (H) Quantification of scratch wound assay (n = 5); (I) Images of tube formation assay (scale bar = 100 μm); (J) Quantification of tube formation assay (n = 5). Data were presented as mean ± SEM. Compared with control group: **P < 0.01, ***P < 0.001. Compared with 1 μM group: # P < 0.05, ##P < 0.01. Compared with CM + CON group: ^^^P < 0.001.

Application: IF/ICC    Species: Rat    Sample:

Figure 2. POL promoted BMSCs-mediated angiogenesis. (A-B) The protein expression levels of VEGFA and CD31 were evaluated by western blot (n = 3); (C-D) Immunofluorescence staining of VEGFA and CD31 (scale bar = 200 μm); (E) Images of transwell migration assay (scale bar = 100 μm); (F) Quantification of transwell migration assay (n = 5); (G) Images of scratch wound assay (scale bar = 100 μm); (H) Quantification of scratch wound assay (n = 5); (I) Images of tube formation assay (scale bar = 100 μm); (J) Quantification of tube formation assay (n = 5). Data were presented as mean ± SEM. Compared with control group: **P < 0.01, ***P < 0.001. Compared with 1 μM group: # P < 0.05, ##P < 0.01. Compared with CM + CON group: ^^^P < 0.001.
10). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2025 (PubMed: 39248296) [IF=12.5]
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