4E-BP1 Antibody - #AF6431

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产品: 4E-BP1 抗体
货号: AF6431
描述: Rabbit polyclonal antibody to 4E-BP1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q13541
RRID: AB_2835047

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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
4E-BP1 Antibody detects endogenous levels of total 4E-BP1.
RRID:
AB_2835047
引用格式: Affinity Biosciences Cat# AF6431, RRID:AB_2835047.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human 4E-BP1, corresponding to a region within the internal amino acids.

基因/基因ID:
EIF4EBP1(1978)
描述:
4E-BP1 binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

文献引用

1). Protective effects and potential mechanisms of (2-Carboxyethyl) dimethylsulfonium Bromide (Br-DMPT) on gill health status of on-growing grass carp (Ctenopharyngodon idella) after infection with Flavobacterium columnare. FISH & SHELLFISH IMMUNOLOGY, 2020 (PubMed: 32771611) [IF=4.1]

Application: WB    Species: grass carp    Sample: gill

Fig. 8. |Western blot analysis of TOR protein phosphorylation (p-TOR Ser2448) (A), S6K1 protein phosphorylation (p-S6K1 Thr-389) (B), 4EBP1 protein phosphorylation (Thr-37/46) (C) in the gill of on-growing grass carp fed diets containing graded levels of Br-DMPT for 60 days, then infected with F. columnare for 3 days.Data represent means of six fish in each group, error bars indicate S.D. Values having different letters are significantly different (P < 0.05).
2). Qi Dan Li Xin pill improves chronic heart failure by regulating mTOR/p70S6k-mediated autophagy and inhibiting apoptosis. Scientific Reports, 2020 (PubMed: 32269242) [IF=3.8]

Application: WB    Species: rat    Sample:

Figure 8. (Fan). |Protein amounts of mTOR pathway efectors. (A) Representative immunoblot bands of various proteins.

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