AR Antibody - #AF6137

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(6)   (5)  
产品: AR 抗体
货号: AF6137
描述: Rabbit polyclonal antibody to AR
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Rabbit, Dog
蛋白号: P10275
RRID: AB_2835020

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
AR Antibody detects endogenous levels of total Androgen Receptor.
RRID:
AB_2835020
引用格式: Affinity Biosciences Cat# AF6137, RRID:AB_2835020.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AIS; ANDR_HUMAN; Androgen nuclear receptor variant 2; Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease); Androgen receptor; androgen receptor splice variant 4b; AR; AR8; DHTR; Dihydro testosterone receptor; Dihydrotestosterone receptor (DHTR); Dihydrotestosterone receptor; HUMARA; HYSP1; KD; Kennedy disease (KD); NR3C4; Nuclear receptor subfamily 3 group C member 4 (NR3C4); Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy (SBMA); Spinal and bulbar muscular atrophy; Testicular Feminization (TFM); TFM;

抗原和靶标

免疫原:

A synthesized peptide derived from human AR, corresponding to a region within the internal amino acids.

基因/基因ID:
AR(367)
描述:
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

文献引用

1). MiR26a reverses enzalutamide resistance in a bone-tumor targeted system with an enhanced effect on bone metastatic CRPC. Journal of nanobiotechnology, 2024 (PubMed: 38566211) [IF=10.2]

Application: WB    Species: Mouse    Sample:

Fig. 6 In vivo safety and bone protection of Bm@PT/Enz-miR26a in two models. (A–B) The H&E images of heart, liver, spleen, lung, kidney, tumor, and bone (left hind limb tibia) of each group in (A) subcutaneous CRPC and (B) BmCRPC mouse models. Scale bar: 100 μm. Red arrow: tumor metastatic sites. (C–D). The biochemical analysis of mouse blood samples in (C) subcutaneous CRPC and (D) BmCRPC mouse models. n = 3, mean ± SD. (E) Metastasis in the heart and lungs of tumor-bearing mice in BmCRPC model. Yellow circle: tumor. (F) The micro-CT images of each group in the BmCRPC mouse model. n = 5, red arrows: bone damaged sites. (G–H) The miR26a level of each group in (G) subcutaneous CRPC and (H) BmCRPC mouse models. n = 3, mean ± SD, ****p 
2). Cell-free fat extract restores hair loss: a novel therapeutic strategy for androgenetic alopecia. Stem Cell Research & Therapy, 2023 (PubMed: 37612726) [IF=7.5]

Application: WB    Species: Human    Sample: hDPCs cells

Fig. 5 Effect of CEFFE (0, 50, 250, 500 μg/ml; 72 h), DHT (1, 10, 100 μM; 72 h) and DHT + CEFFE (100 μM DHT + 50, 250, 500 μg/ml CEFFE; 72 h) on the intracellular DHT concentration and AR expression of hDPCs. A Quantitative analysis of DHT concentration using ELISA. B RT-qPCR analysis of AR mRNA expression after culturing hDPCs with CEFFE, DHT and DHT + CEFFE, respectively. qRT-PCR analysis showed the expression of AR was increased after DHT treatment but declined after co-incubation with DHT and CEFFE. C Western blot for AR after treating hDPCs with CEFFE, DHT and DHT + CEFFE. Original data was presented in Additional file 2: Fig. 4. It revealed an increase in the expression of AR after incubating with DHT but decreased after co-incubation with DHT and CEFFE. Data represent the mean ± SD; n = 3; *P 
3). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology, 2020 (PubMed: 32457626) [IF=5.6]

Application: IHC    Species: mouse    Sample: tumor

Supplementary Figure 2. |Immunohistochemical examination of 17βHSD3 and AR expression in tumor tissue. (A) Immunohistochemical labeling of 17βHSD3-positive cells. Arrows indicate 17βHSD3 expression.(C) Quantitative analysis of 17βHSD3-expressing cells using ImageJ software. (B) Immunohistochemical labeling of AR-positive cells. Arrows indicate AR expression.
4). Hsa_circ_0102485 inhibits the growth of cancer cells by regulating the miR-188-3p/ARID5B/AR axis in prostate carcinoma. Pathology, research and practice, 2022 (PubMed: 35939970) [IF=2.9]
5). Naftopidil enantiomers suppress androgen accumulation and induce cell apoptosis via the UDP-glucuronosyltransferase 2B15 in benign prostate hyperplasia. The Journal of steroid biochemistry and molecular biology, 2022 (PubMed: 35504423) [IF=2.7]
6). The miR-34b/MEK/ERK pathway is regulated by NR5A1 and promotes differentiation in primary bovine Sertoli cells. Theriogenology, 2024 (PubMed: 38100994) [IF=2.4]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.