IGF1R/Insulin Receptor Antibody - #AF6125

>>奖励金 进行中>>
(11)   (6)  
产品: IGF1R/Insulin Receptor 抗体
货号: AF6125
描述: Rabbit polyclonal antibody to IGF1R/Insulin Receptor
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Rabbit, Dog, Chicken, Xenopus
蛋白号: P08069 | P06213
RRID: AB_2835009

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IGF1R/Insulin Receptor Antibody detects endogenous levels of total IGF1R/Insulin Receptor.
RRID:
AB_2835009
引用格式: Affinity Biosciences Cat# AF6125, RRID:AB_2835009.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF I receptor; IGF-I receptor; Igf1r; IGF1R_HUMAN; IGFIR; IGFIRC; IGFR; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; Soluble IGF1R variant 1; Soluble IGF1R variant 2; CD220; HHF5; HIR B; INSR; Insulin receptor; Insulin receptor subunit beta; IR;

抗原和靶标

免疫原:

A synthesized peptide derived from human IGF1R/Insulin Receptor, corresponding to a region within C-terminal amino acids.

基因/基因ID:
IGF1R(3480) | INSR(3643)
描述:
Insulin-like growth factor I, or IGF-I, is an ubiquitous peptide that acts in both an autocrine and paracrine fashion to stimulate the growth of vascular smooth muscle cells. In addition, IGF-I regulates renal function, growth and repair, is critically involved in bone formation and resorption and has been implicated in mediating aspects of the immune response.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). Tumor-associated macrophages/C-X-C motif chemokine ligand 1 promotes breast cancer autophagy-mediated chemoresistance via IGF1R/STAT3/HMGB1 signaling. Cell death & disease, 2024 (PubMed: 39394189) [IF=8.1]

Application: WB    Species: Mouse    Sample: MDA-MB-231 cells

Fig. 5: Identification of IGF1/IGF1R as a downstream signaling for CXCL1-induced autophagy.A The heatmap and Venn diagram analyses were conducted using data from a high-throughput quantitative PCR chip to evaluate differences among the indicated four groups, with a focus on IGF1 as the primary target. B Western blotting analysis was performed to evaluate the impact of varying concentrations of CXCL1 (0–50 ng/mL), CXCL1 overexpression, or CXCL1 knockdown on the expression levels of IGF1 and its receptor IGF1R in MDA-MB-231 cells. C Immunofluorescence was utilized to examine the expression and distribution of IGF1R with 20 ng/mL CXCL1 alone or its combination with 40 ng/mL IGF1 (scale bars indicate 20 μm). D, E mRFP-GFP-LC3 reporter assay was conducted to investigate the effect of 40 ng/mL IGF1 or IGF1R silence on CXCL1-mediated autophagy in MDA-MB-231 cells (n = 5, scale bars indicate 20 μm). F CoIP analysis was conducted to assess the effect of CXCL1 treatment (20 ng/mL) on protein interaction between IGF1 and IGF1R in MDA-MB-231 cells. G, H Western blotting assay was utilized to examine whether the change of IGF1R expression was affected by proteasome degradation. CXCL1 dose was 20 ng/mL, IGF1 dose was 40 ng/mL, CHX dose was 10 μM, and MG132 dose was 10 μM. Values are presented as Mean ± SD, n = 3 unless otherwise indicated, *P 
2). IGF1R Promotes Th17/Treg Cell Development in Experimental Autoimmune Prostatitis. Journal of inflammation research, 2025 (PubMed: 40322534) [IF=4.5]

Application: IF/ICC    Species: Mouse    Sample:

Figure 2 IGF1-related ligands and IGF1R’ s expression in EAP and Control groups. (A) The gene expressions of IGF1-related ligands Igf1r, Igfbp4, and Igf2r were quantified using qPCR in the prostate tissues of both the EAP group and the Control group (n=5); (B) Flow cytometry was employed to screen Th1, Th2, Th17, and Treg cells while assessing the expression levels of Igf1r, Igfbp4, and Igf2r in these cell types (n=5); (C) The ratio of Th17 cells in the spleen of EAP group and Control group was detected by flow cytometry (n=5). (D) The ratio of Treg cells in the spleen of EAP group and Control group was detected by flow cytometry (n=5). (E) The expression of IGF1R in the prostate tissue of two group was detected by WB; (F) The expression of IGF1R in the prostate of two group was detected by IHC (400X). (G) Immunofluorescence co-localization experiments were conducted to observe the subcellular localization of the IGF1R protein within the prostate tissue of mouse in the EAP group and to identify the cell types with high IGF1R expression (1000X).
3). Sodium butyrate alleviates right ventricular hypertrophy in pulmonary arterial hypertension by inhibiting H19 and affecting the activation of let-7g-5p/IGF1 receptor/ERK. European journal of pharmacology, 2024 (PubMed: 38176636) [IF=4.2]
4). Ropivacaine suppresses tumor biological characteristics of human hepatocellular carcinoma via inhibiting IGF-1R/PI3K/AKT/mTOR signaling axis. Bioengineered, 2021 (PubMed: 34696683) [IF=4.2]
5). Rhizoma Drynariae-derived EV-like particles alleviate osteoporosis by promoting osteogenic differentiation in BMSCs through the activation of the hsa_circ_0001275/miR-422a pathway. Bone, 2025 (PubMed: 40239729) [IF=3.5]
6). Human umbilical cord mesenchymal stem cells protect against ferroptosis in acute liver failure through the IGF1-hepcidin-FPN1 axis and inhibiting iron loading. Acta biochimica et biophysica Sinica, 2024 (PubMed: 38273781) [IF=3.3]

Application: IHC    Species: Mouse    Sample:

Figure 5 . The role of IGF1 in ALF mice with MSC treatment (A) Immunofluorescence staining of the macrophage marker CD11b in the liver. The magnification is 1000× and 3000×. (B) Serum IGF1 expression was detected by ELISA. (C) Immunohistochemical staining of IGF-1R proteins in the NC, CCL4+PBS, and CCL4+MSC groups (n=3). The magnification is 100×. (D) The survival curves of mice in each group. (E,F) The levels of MDA and GSH were measured. (G) Immunolocalization of Ptgs2 and Gpx4 proteins in the NC, CCL4+PBS, and CCL4+MSC groups (n=3). The magnification is 100×. Data are expressed as the mean±SEM. ns: no significant difference. *P
7). Overexpression of miR-297b-5p in Mouse Insulin-Secreting Cells Promotes Metformin-Mediated Protection Against Stearic Acid-Induced Senescence by Targeting Igf1r. Frontiers in Bioscience-Landmark, 2023 (PubMed: 37664932) [IF=3.3]
8). Capsaicin reduces blood glucose and prevents prostate growth by regulating androgen, RAGE/IGF-1/Akt, TGF-β/Smad signalling pathway and reversing epithelial-mesenchymal transition in streptozotocin-induced diabetic mice. Naunyn-Schmiedeberg's archives of pharmacology, 2024 (PubMed: 38700794) [IF=3.1]
9). IGF1R activates FOXP3-β-catenin signaling to promote breast cancer development. Breast cancer research and treatment, 2025 (PubMed: 40055251) [IF=3.0]
10). Overexpression of miR-297b-5p Promotes Metformin-Mediated Protection Against Stearic Acid-Induced Senescence by Targeting Igf1r. , 2022
加载更多

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.