产品: IKK alpha 抗体
货号: AF6012
描述: Rabbit polyclonal antibody to IKK alpha
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 85kDa; 85kD(Calculated).
蛋白号: O15111
RRID: AB_2834946

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
克隆:
Polyclonal
特异性:
IKK alpha Antibody detects endogenous levels of total IKK alpha.
RRID:
AB_2834946
引用格式: Affinity Biosciences Cat# AF6012, RRID:AB_2834946.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

chuk; CHUK1; Conserved Helix Loop Helix Ubiquitous Kinase; Conserved helix loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I Kappa B Kinase 1; I Kappa B Kinase Alpha; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK 1; IKK A; IKK a kinase; IKK-A; IKK-alpha; IKK1; IKKA; IKKA_HUMAN; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear Factor Kappa B Inhibitor Kinase Alpha; Nuclear factor NF kappa B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor; TCF-16; TCF16; Transcription factor 16;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O15111 IKKA_HUMAN:

Widely expressed.

描述:
IKK-alpha a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively.
序列:
MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
91
Chicken
91
Sheep
88
Xenopus
82
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O15111 作为底物

Site PTM Type Enzyme
T23 Phosphorylation Q9Y243 (AKT3) , O00141 (SGK1) , P31749 (AKT1) , P31751 (AKT2)
K44 Ubiquitination
K53 Ubiquitination
K67 Ubiquitination
K105 Ubiquitination
K109 Ubiquitination
K117 Ubiquitination
S119 Acetylation
K139 Ubiquitination
K146 Ubiquitination
K158 Ubiquitination
Y168 Phosphorylation
S176 Phosphorylation Q04759 (PRKCQ) , Q99558 (MAP3K14)
T179 Acetylation
S180 Phosphorylation Q04759 (PRKCQ) , Q99558 (MAP3K14)
K296 Ubiquitination
K322 Acetylation
K366 Ubiquitination
K391 Ubiquitination
K415 Ubiquitination
K438 Acetylation
T463 Phosphorylation
S473 Phosphorylation Q13315 (ATM)
Y500 Phosphorylation
K506 Ubiquitination
S536 Phosphorylation
K569 Ubiquitination
K572 Ubiquitination
S576 Phosphorylation
T593 Phosphorylation
K602 Ubiquitination
K615 Ubiquitination
K617 Ubiquitination
K624 Ubiquitination
T722 Acetylation

翻译修饰 - O15111 作为激酶

Substrate Site Source
O14920-1 (IKBKB) S177 Uniprot
O14920-1 (IKBKB) S181 Uniprot
O43524 (FOXO3) S644 Uniprot
O75925-1 (PIAS1) S90 Uniprot
P03372 (ESR1) S118 Uniprot
P19838-2 (NFKB1) S922 Uniprot
P19838 (NFKB1) S923 Uniprot
P19838-2 (NFKB1) S924 Uniprot
P19838 (NFKB1) S927 Uniprot
P19838-2 (NFKB1) S928 Uniprot
P19838 (NFKB1) T931 Uniprot
P19838 (NFKB1) S932 Uniprot
P19838-2 (NFKB1) T932 Uniprot
P19838-2 (NFKB1) S933 Uniprot
P24385 (CCND1) T286 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P35222 (CTNNB1) S33 Uniprot
P35222 (CTNNB1) S37 Uniprot
P35222 (CTNNB1) T41 Uniprot
P35222 (CTNNB1) S45 Uniprot
P42345 (MTOR) S1415 Uniprot
P84243 (H3F3B) S11 Uniprot
Q00653 (NFKB2) S99 Uniprot
Q00653 (NFKB2) S108 Uniprot
Q00653 (NFKB2) S115 Uniprot
Q00653 (NFKB2) S123 Uniprot
Q00653 (NFKB2) S872 Uniprot
Q01094 (E2F1) S403 Uniprot
Q01201 (RELB) S472 Uniprot
Q04206 (RELA) S536 Uniprot
Q04864 (REL) S557 Uniprot
Q14164 (IKBKE) S172 Uniprot
Q15653-1 (NFKBIB) S19 Uniprot
Q15653-1 (NFKBIB) S23 Uniprot
Q15717 (ELAVL1) S304 Uniprot
Q5S007 (LRRK2) S910 Uniprot
Q5S007 (LRRK2) S935 Uniprot
Q86VP1-2 (TAX1BP1) S593 Uniprot
Q86VP1-2 (TAX1BP1) S624 Uniprot
Q86VP1 (TAX1BP1) S666 Uniprot
Q92793 (CREBBP) S1382 Uniprot
Q92793 (CREBBP) S1386 Uniprot
Q99558 (MAP3K14) T559 Uniprot
Q9BUZ4 (TRAF4) S426 Uniprot
Q9BXH1 (BBC3) S10 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9NQC7 (CYLD) S422 Uniprot
Q9NQC7 (CYLD) S432 Uniprot
Q9NQC7 (CYLD) S436 Uniprot
Q9NQC7 (CYLD) S439 Uniprot
Q9NQC7 (CYLD) S441 Uniprot
Q9NQC7 (CYLD) S444 Uniprot
Q9Y261 (FOXA2) S107 Uniprot
Q9Y261 (FOXA2) S111 Uniprot
Q9Y6Q9-5 (NCOA3) S857 Uniprot

研究背景

功能:

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity).

翻译修饰:

Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.

(Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

细胞定位:

Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65 (By similarity). Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14. Directly interacts with TRPC4AP (By similarity). May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1. Interacts with ARRB1 and ARRB2. Interacts with NLRC5; prevents CHUK phosphorylation and kinase activity. Interacts with PIAS1; this interaction induces PIAS1 phosphorylation. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner. Interacts with FOXO3. Interacts with IFIT5; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation. Interacts with LRRC14. Interacts with SASH1.

(Microbial infection) Interacts with InlC of Listeria monocytogenes.

蛋白家族:

The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Duan Z et al. The matrix protein of Newcastle disease virus inhibits inflammatory response through IRAK4/TRAF6/TAK1/NF-κB signaling pathway. International Journal of Biological Macromolecules 2022 Oct 1;218:295-309. (PubMed: 35872314) [IF=8.2]

2). Meng et al. S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1. Oncogene 2020 Jun 17. (PubMed: 32555330) [IF=8.0]

Application: WB    Species: Human    Sample: NPC cells

Fig 4.b NF-KB signaling makers in S100A14 overexpressing cells and S100A14 knocked-down cells were evaluated by immunoblotting.

3). Isolation and identification of immunomodulatory peptides from the protein hydrolysate of tuna trimmings (Thunnas albacares). LWT [IF=6.0]

4). Chen X et al. Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation. JOURNAL OF CELLULAR PHYSIOLOGY 2018 Dec 10 (PubMed: 30536538) [IF=5.6]

Application: WB    Species: human    Sample: HUVECs cells

FIGURE 3| Gal‐3 promotes ox‐LDL‐induced inflammatory responses via NF‐κB activation and enhances the expression of related inflammatory factors, chemokines, and adhesion molecules. After exposure to Gal‐3, ox‐LDL or their combination, the total and phosphorylated expression of p65, IKKα, and IKKβ was examined by WB demonstrated by histogram (a and b); the levels of IL‐6, IL‐8, and IL‐1β and chemokines(CXCL‐1 and CCL‐2) were measured by ELISA. (c and d) The expression of VCAM‐1 and ICAM‐1 was detected by WB and the relative protein expression was given by histogram. (c) Each experiment was performed in triplicate. *p < 0.05, **p < 0.01, ##p < 0.01

5). Zhou SS et al. Shenkang VII Recipe Attenuates Unilateral Ureteral Obstruction-induced Renal Fibrosis via TGF-β/Smad, NF-κB and SHH Signaling Pathway. Current Medical Science 2020 Oct;40(5):917-930. (PubMed: 32980902) [IF=2.4]

Application: WB    Species: Rat    Sample: UUO group rats

Fig. 8 SK-7 downregulated the NF-κB signaling pathway in UUO group rats A: Detection of p-NF-κB, NF-κB, p-IKKα, IKKα, p-IκBα and IκBα in kidneys induced by UUO for 7 and 14 days by Western blotting. B–E: Western blot ratio analysis. **P<0.01, UUO group vs. control group; ##P<0.01, SK7-treated groups vs. UUO group

6). The Yi-Qi-Bu-Shen recipe attenuates high glucose-induced podocyte injury via the inhibition of IKK-IκBα-NFκB and ERK/P38 MAPK signaling .

Application: WB    Species: Human    Sample: podocytes

Figure 4. Effect of YB on NFκB signaling pathway in podocytes. A. Podocytes were transfected with NFκB reporter constructs as well as internal control plasmids of pRL-TK, stimulated for 6 hours, and then followed with the assay of NFκB transcriptional activity as described in methods and materials. (*P

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