Phospho-4E-BP1 (Thr45) Antibody - #AF3432

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产品: 磷酸化 4E-BP1 (Thr45) 抗体
货号: AF3432
描述: Rabbit polyclonal antibody to Phospho-4E-BP1 (Thr45)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q13541
RRID: AB_2834874

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-4E-BP1 (Thr45) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Threonine 45.
RRID:
AB_2834874
引用格式: Affinity Biosciences Cat# AF3432, RRID:AB_2834874.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45.

基因/基因ID:
EIF4EBP1(1978)
描述:
4E-BP1 binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

文献引用

1). The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway. International journal of molecular sciences, 2025 (PubMed: 40243924) [IF=5.6]
2). MEK inhibitor trametinib combined with PI3K/mTOR inhibitor BEZ-235 as an effective strategy against NSCLC through impairment of glucose metabolism. Cellular signalling, 2024 (PubMed: 39293743) [IF=4.4]
3). Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors. Aging-US, 2021 (PubMed: 33472171) [IF=3.9]

Application: WB    Species: Rat    Sample: GH3 cell

Figure 5 Effect of anit-DLK1 antibody on the bioactivity of PitNET cell lines. (A) Western blot assay measured the levels of DLK1 and PIT1 in GH3 cell line, MMQ cell line and ATT20 cell line. (B) Anti-DLK1 antibody inhibited the cell viability of GH3 cells in the dose- and time-dependent manner, not MMQ cells or ATT20 cells. (C) Anti-DLK1 antibody inhibited the secretion of GH/IGF-1 in GH3 cells, not PRL in MMQ cells and ACTH in ATT20 cells. (D) Clone forming experiment showed the anti-DLK1 antibody promoted the cell proliferation in GH3 cell line. (E) Confocal experiment showed DLK1 regulated the level of PIT1 in GH3 cell line. (F) Western blot experiment showed Anti-DLK1 antibody activated the mTOR pathway in GH3 cell line. *compare to control group P<0.05 **P<0.01 ***P<0.001.
4). Melatonin alleviates endoplasmic reticulum stress and follicular granulosa cell apoptosis by regulating ATF4 to activate mTOR signaling pathway in chickens. Poultry science, 2024 (PubMed: 38583308) [IF=3.8]
5). Fluoxetine regulates mTOR signalling in a region-dependent manner in depression-like mice. Scientific Reports, 2015 (PubMed: 26522512) [IF=3.8]

Application: WB    Species: mouse    Sample: mouse

Figure 5. Effect of fluoxetine (20mg/kg) and rapamycin (10mg/kg) on the level of phosphorylated-4E-BP-1 expression in the frontal cortex (A), the hippocampus (B), the amygdala (C) and the hypothalamus (D). The data represented the values of mean± S.E.M. from 5mice/group. # P< 0.05 and ##P< 0.01 vs Control-vehicle group. *P< 0.05 and **P< 0.01 vs CUMS-vehicle group. +P< 0.05 vs CUMS-fluoxetine group. The results of Two-way ANOVA are provided in supplemental materials.
6). The Role of Specificity Protein 1 (SP1) in Bladder Cancer Progression through PTEN-Mediated AKT/mTOR Pathway. Urologia internationalis, 2023 (PubMed: 37666229) [IF=1.5]
7). The DLK1/MEG3 Locus is Related to the Differentiation of Pituitary Neuroendocrine Tumors. Research Square, 2020

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