Phospho-ACC1 (Ser79)[Ser80] Antibody - #AF3421

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产品: 磷酸化 ACC1 (Ser79)[Ser80] 抗体
货号: AF3421
描述: Rabbit polyclonal antibody to Phospho-ACC1 (Ser79)[Ser80]
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q13085
RRID: AB_2834863

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 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-ACC1 (Ser79) Antibody detects endogenous levels of ACC1 only when phosphorylated at Ser80, which site historically referenced as Ser79.
RRID:
AB_2834863
引用格式: Affinity Biosciences Cat# AF3421, RRID:AB_2834863.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACAC;ACACA;ACACA_HUMAN;ACC alpha;ACC 1;ACC-alpha;ACC1;ACCA;Acetyl CoA carboxylase 1;Acetyl CoA carboxylase alpha;Acetyl Coenzyme A carboxylase alpha;Biotin carboxylase;COA1;HACC275 antibody;

抗原和靶标

免疫原:

A synthesized peptide derived from human ACC1 around the phosphorylation site of Ser79.

基因/基因ID:
ACACA(31)
描述:
ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Comprehensive multi-omics approaches reveal the hepatotoxic mechanism of perfluorohexanoic acid (PFHxA) in mice. Science of The Total Environment, 2021 (PubMed: 34380288) [IF=8.2]

Application: IHC    Species: Mice    Sample: liver tissue

Fig. 4. Expression analysis of genes and proteins associated with fatty acid metabolism in mice with PFHxA exposure. (A) Expression of fatty acid biosynthesis genes, (B) Expression of fatty acid peroxisomal oxidation genes, (C) Expression of genes related to inflammation. (D–G) IHC staining of FASN (D), p-ACAC (E), EHHADH (F) and p-mTOR (G). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (n = 3–4).
2). Deletion of PPARα in mouse brown adipocytes increases their De Novo Lipogenesis. Molecular metabolism, 2025 (PubMed: 40499650) [IF=7.0]

Application: WB    Species: Mouse    Sample:

Figure 4. BAT specific deletion of PPARα increases BAT De Novo Lipogenesis for fat storage in HFD-induced obese mice upon β3-adrenergic stimulation. (A, G) Relative mRNA levels of De Novo Lipogenesis (DNL) genes in brown adipose tissue (BAT) of female (A) and male (G) mice (n = 4–6 per group). (B) Western blots of DNL proteins in BAT of female mice and corresponding quantifications (C) (n = 3 per group). (D, H) Relative abundancy of palmitate and lipogenic index in BAT triglycerides (TG) of female (D) and male (H) mice (n = 4 per group). (E) Western blot of PKM2 protein in BAT of female mice and corresponding quantification (n = 3 per group). (F) Western blot of AKT2 protein in BAT of female mice and corresponding quantification (n = 3 per group). (I) Relative fatty acid content in BAT triglycerides compared to internal standard C19 of male mice (n = 4 per group). (J) Relative mRNA levels of Pkm2 in BAT of male mice (n = 4–6 per group). Data are displayed as mean ± SEM. Statistical analysis was performed using 2-way ANOVA with Šídák’s post hoc test or Mann–Whitney test (A for Chrebpb, Acly, Fasn, and G, Chrebpb, Acly, Acaca, Fasn). ∗p < 0.05, vs Ctrl. £p < 0.05, vs NaCl. NaCl: vehicle; CL: CL316,243 (β3-adrenergic agonist). Ctrl: control mice; KO: PPARαBATKO mice.
3). Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells. Biomedicine & Pharmacotherapy, 2020 (PubMed: 33152903) [IF=6.9]
4). α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube. Nutrients, 2021 (PubMed: 34371902) [IF=5.9]

Application: WB    Species: Mice    Sample:

Figure 2 The effects of HICA on the intracellular signaling pathways. A typical image for a capillary immunoassay is shown (A). The phosphorylation levels of (B) p70S6K and 4E-BP1; (C) AMPK, ACC, and ULK1; (D) ERK1/2; (E) p38MAPK; and (F) eEF2 are shown. The phosphorylation is normalized to the total protein expression. The β-tubulin content in the lysate was measured as a loading control (G). The time course of these experiments is shown in the upper region. DM: differentiation medium, DMEM: Dulbecco’s modified Eagle’s medium, and w/o AA: without amino acids. Data are displayed as the means ± SD, and n = 4 for each group in all bar graphs. * p < 0.05 and ** p < 0.01 vs. the vehicle-treated group.
5). Amphibian pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell nutrient deficiency. iScience, 2022 (PubMed: 37128610) [IF=5.8]
6). Liraglutide Alleviates Hepatic Steatosis and Liver Injury in T2MD Rats via a GLP-1R Dependent AMPK Pathway. Frontiers in Pharmacology, 2021 (PubMed: 33746742) [IF=5.6]
7). Vitisin A Outperforms Cyanidin-3-O-Glucoside in Triglyceride Reduction by Modulating Hepatic Lipogenesis and Fatty Acid β-Oxidation. International journal of molecular sciences, 2025 (PubMed: 40003987) [IF=5.6]
8). Ginsenoside CK ameliorates hepatic lipid accumulation via activating the LKB1/AMPK pathway in vitro and in vivo. Food & Function, 2022 (PubMed: 35018944) [IF=5.1]
9). Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells. Journal of Functional Foods, 2021 [IF=3.8]
10). Cai's herbal tea enhances mitochondrial autophagy of type 1 diabetic mellitus β cells through the AMPK/mTOR pathway and alleviates inflammatory response. Acta diabetologica, 2024 (PubMed: 38954041) [IF=3.1]
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