产品: 磷酸化 ACC1 (Ser79)[Ser80] 抗体
货号: AF3421
描述: Rabbit polyclonal antibody to Phospho-ACC1 (Ser79)[Ser80]
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 280kDa; 266kD(Calculated).
蛋白号: Q13085
RRID: AB_2834863

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(85%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(80%)
克隆:
Polyclonal
特异性:
Phospho-ACC1 (Ser79) Antibody detects endogenous levels of ACC1 only when phosphorylated at Ser80, which site historically referenced as Ser79.
RRID:
AB_2834863
引用格式: Affinity Biosciences Cat# AF3421, RRID:AB_2834863.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACAC;ACACA;ACACA_HUMAN;ACC alpha;ACC 1;ACC-alpha;ACC1;ACCA;Acetyl CoA carboxylase 1;Acetyl CoA carboxylase alpha;Acetyl Coenzyme A carboxylase alpha;Biotin carboxylase;COA1;HACC275 antibody;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q13085 ACACA_HUMAN:

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

描述:
ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis.
序列:
MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
92
Rabbit
92
Zebrafish
85
Chicken
80
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13085 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
S23 Phosphorylation
S25 Phosphorylation
S29 Phosphorylation
S34 Phosphorylation
K45 Ubiquitination
S48 Phosphorylation
S50 Phosphorylation
S53 Phosphorylation
S56 Phosphorylation
T58 Phosphorylation
S60 Phosphorylation
S77 Phosphorylation P17612 (PRKACA)
S78 Phosphorylation P54646 (PRKAA2)
S80 Phosphorylation P54646 (PRKAA2) , Q13131 (PRKAA1)
K86 Ubiquitination
S96 Phosphorylation
S104 Phosphorylation
K116 Ubiquitination
K120 Ubiquitination
K132 Ubiquitination
S234 Phosphorylation
K276 Ubiquitination
K288 Ubiquitination
Y290 Phosphorylation
K292 Ubiquitination
K311 Ubiquitination
S344 Phosphorylation
K386 Ubiquitination
K410 Ubiquitination
Y417 Phosphorylation
Y434 Phosphorylation
S488 Phosphorylation
K520 Ubiquitination
K579 Ubiquitination
T610 Phosphorylation
Y676 Phosphorylation
Y734 Phosphorylation
K747 Ubiquitination
S756 Phosphorylation
K817 Ubiquitination
K825 Ubiquitination
S835 Phosphorylation
S841 Phosphorylation
Y855 Phosphorylation
Y867 Phosphorylation
K879 Ubiquitination
S893 Phosphorylation
S907 Phosphorylation
K916 Ubiquitination
S977 Phosphorylation
Y994 Phosphorylation
T1042 Phosphorylation
T1073 Phosphorylation
K1076 Ubiquitination
T1196 Phosphorylation
S1201 Phosphorylation P17612 (PRKACA)
Y1209 Phosphorylation
S1216 Phosphorylation
S1218 Phosphorylation
T1227 Phosphorylation
S1257 Phosphorylation
S1259 Phosphorylation
S1263 Phosphorylation
T1265 Phosphorylation
S1273 Phosphorylation
K1316 Ubiquitination
K1334 Acetylation
K1334 Ubiquitination
K1338 Ubiquitination
Y1342 Phosphorylation
K1354 Ubiquitination
Y1370 Phosphorylation
K1435 Ubiquitination
S1438 Phosphorylation
Y1441 Phosphorylation
Y1499 Phosphorylation
K1524 Ubiquitination
K1580 Acetylation
K1586 Ubiquitination
S1720 Phosphorylation
Y1745 Phosphorylation
K1759 Acetylation
K1759 Ubiquitination
K1781 Ubiquitination
K1788 Acetylation
K1788 Ubiquitination
S1844 Phosphorylation
K1856 Acetylation
K1856 Ubiquitination
S1908 Phosphorylation
K1916 Ubiquitination
K1929 Ubiquitination
K2031 Acetylation
K2031 Ubiquitination
R2047 Methylation
S2099 Phosphorylation
Y2108 Phosphorylation
K2127 Acetylation
K2127 Ubiquitination
K2135 Ubiquitination
Y2144 Phosphorylation
T2153 Phosphorylation
K2209 Ubiquitination
K2231 Ubiquitination
K2268 Ubiquitination
K2293 Ubiquitination
K2302 Ubiquitination
S2318 Phosphorylation
S2343 Phosphorylation
S2345 Phosphorylation

研究背景

功能:

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.

翻译修饰:

Phosphorylation on Ser-1263 is required for interaction with BRCA1.

Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.

The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.

细胞定位:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

亚基结构:

Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.

蛋白家族:

Consists of an N-terminal biotin carboxylation/carboxylase (BC) domain that catalyzes the ATP-dependent transient carboxylation of the biotin covalently attached to the central biotinyl-binding/biotin carboxyl carrier (BCC) domain (Probable). The C-terminal carboxyl transferase (CT) domain catalyzes the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA to produce malonyl-CoA (Probable).

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Comprehensive multi-omics approaches reveal the hepatotoxic mechanism of perfluorohexanoic acid (PFHxA) in mice. Science of The Total Environment, 2021 (PubMed: 34380288) [IF=9.8]

Application: IHC    Species: Mice    Sample: liver tissue

Fig. 4. Expression analysis of genes and proteins associated with fatty acid metabolism in mice with PFHxA exposure. (A) Expression of fatty acid biosynthesis genes, (B) Expression of fatty acid peroxisomal oxidation genes, (C) Expression of genes related to inflammation. (D–G) IHC staining of FASN (D), p-ACAC (E), EHHADH (F) and p-mTOR (G). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (n = 3–4).

2). Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells. Biomedicine & Pharmacotherapy, 2020 (PubMed: 33152903) [IF=7.5]

3). Ginsenoside CK ameliorates hepatic lipid accumulation via activating the LKB1/AMPK pathway in vitro and in vivo. Food & Function, 2022 (PubMed: 35018944) [IF=6.1]

4). α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube. Nutrients, 2021 (PubMed: 34371902) [IF=5.9]

Application: WB    Species: Mice    Sample:

Figure 2 The effects of HICA on the intracellular signaling pathways. A typical image for a capillary immunoassay is shown (A). The phosphorylation levels of (B) p70S6K and 4E-BP1; (C) AMPK, ACC, and ULK1; (D) ERK1/2; (E) p38MAPK; and (F) eEF2 are shown. The phosphorylation is normalized to the total protein expression. The β-tubulin content in the lysate was measured as a loading control (G). The time course of these experiments is shown in the upper region. DM: differentiation medium, DMEM: Dulbecco’s modified Eagle’s medium, and w/o AA: without amino acids. Data are displayed as the means ± SD, and n = 4 for each group in all bar graphs. * p < 0.05 and ** p < 0.01 vs. the vehicle-treated group.

5). Amphibian pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell nutrient deficiency. iScience, 2022 (PubMed: 37128610) [IF=5.8]

6). Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells. Journal of Functional Foods, 2021 [IF=5.6]

7). Liraglutide Alleviates Hepatic Steatosis and Liver Injury in T2MD Rats via a GLP-1R Dependent AMPK Pathway. Frontiers in Pharmacology, 2021 (PubMed: 33746742) [IF=5.6]

8). Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid. The British journal of nutrition, 2022 (PubMed: 33910655) [IF=3.6]

Application: WB    Species: Crab    Sample: hepatopancreas tissues

Fig. 5. Effects of dietary myo-inositol on protein levels of p-IP3R, p-AMPK and p-ACC1 of Eriocheir sinensis fed different lipid level. Values are means (n=4 replicate tanks) with standard errors represented by vertical bars. * indicates significant difference between myo-inositol levels within the same lipid level or between lipid levels within the same myo-inositol level (P < 0.05). p-IP3R, phosphorylation of inositol 1,4,5-trisphosphate receptor; p-AMPK, phosphorylation of adenosine 5’-monophosphate-activated protein kinase; p-ACC1, phosphorylation of acetyl-CoA carboxylase 1.

9). Liraglutide regulates lipid metabolism via FGF21-LKB1-AMPK-ACC1 pathway in white adipose tissues and macrophage of type 2 diabetic mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 33640604) [IF=3.1]

Application: WB    Species: mice    Sample: White adipose tissue (WAT)

Fig. 2. Effects of LRG on adipocyte size and the protein expression in WAT of diabetic mice. (A) Adipocyte size of the mice; (B) Quantification of Fig. 2A; (C) Immunohistochemical assay of WAT; (D) Quantification of Fig. 2C; (E) Protein expression in WAT; (F) Quantification of Fig. 3E n ¼ 6 mice/group. #p < 0.05, ##p < 0.01,###p < 0.001 vs CON; *p < 0.05,**p < 0.01, ***p < 0.001 vs DM.

10). Transcription factor EB enhances autophagy and ameliorates palmitate-induced insulin resistance at least partly via upregulating AMPK activity in skeletal muscle cells. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2022 (PubMed: 34614219) [IF=2.9]

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