Phospho-pan-FOXO1/3/4 (Thr24/Thr32) Antibody - #AF3419

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产品: 磷酸化 总FOXO1/3/4 (Thr24/Thr32) 抗体
货号: AF3419
描述: Rabbit polyclonal antibody to Phospho-pan-FOXO1/3/4 (Thr24/Thr32)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Dog, Chicken, Xenopus
蛋白号: Q12778 | P98177
RRID: AB_2834861

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-pan-FOXO1/3/4 (Thr24/Thr32) Antibody detects endogenous levels of pan-FOXO1/3/4 only when phosphorylated at Threonine 24/32.
RRID:
AB_2834861
引用格式: Affinity Biosciences Cat# AF3419, RRID:AB_2834861.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FKH 1; FKH1; FKHR; Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma); Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FoxO transcription factor; foxo1; FOXO1_HUMAN; FOXO1A; OTTHUMP00000018301; AFX; AFX1; Afxh; ALL1-fused gene from X chromosome; Fork head domain transcription factor AFX1; Forkhead box O4; Forkhead box protein O4; FOXO 4; Foxo4; FOXO4_HUMAN; MGC117660; MGC120490; Mixed lineage leukemia, translocated to, 7; MLLT7; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7; RGD1561201;

抗原和靶标

免疫原:

A synthesized peptide derived from human pan-FOXO1/3/4 around the phosphorylation site of Thr24/32.

基因/基因ID:
FOXO1(2308) | FOXO4(4303)
描述:
FOXO4 transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). FoxO1 is a negative regulator of neointimal hyperplasia in a rat model of patch angioplasty. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37542853) [IF=6.9]
2). Caffeic acid dimethyl ether alleviates alcohol-induced hepatic steatosis via microRNA-378b-mediated CaMKK2-AMPK pathway. Bioengineered, 2022 (PubMed: 35481488) [IF=4.2]

Application: WB    Species: mouse    Sample: livers

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice. (a,b) Western blot analysis of the protein expressions of SREBP-1c, FASN, PPARα, CPT1, MTTP and protein ratio of p-foxO1/foxO1 when the mice were injected with AAV-miR-378b agomir and antagomir. All data are expressed as the means ± SD of at least three separate experiments.
3). 1, 3-dichloro-2-propanol induced hepatic lipid accumulation by inhibiting autophagy via AKT/mTOR/FOXO1 pathway in mice. FOOD AND CHEMICAL TOXICOLOGY, 2021 (PubMed: 34560177) [IF=3.9]
4). Runjing Decoction alleviated cyclophosphamide-induced oligoasthenospermia rats by inhibiting cell apoptosis via RXFP1/AKT/FOXO1 pathway. Andrologia, 2021 (PubMed: 34396564) [IF=2.1]

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