Phospho-HSL (Ser563)[Ser853] Antibody - #AF2350

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产品: 磷酸化 HSL (Ser563)[Ser853] 抗体
货号: AF2350
描述: Rabbit polyclonal antibody to Phospho-HSL (Ser563)[Ser853]
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q05469
RRID: AB_2845364

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-HSL (Ser563) Antibody detects endogenous levels of HSL only when phosphorylated at Ser853, which site historically referenced as Ser563.
RRID:
AB_2845364
引用格式: Affinity Biosciences Cat# AF2350, RRID:AB_2845364.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human HSL around the phosphorylation site of Ser563.

基因/基因ID:
LIPE(3991)

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

文献引用

1). Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting. Food Chemistry, 2020 (PubMed: 32454271) [IF=8.5]

Application: WB    Species: Pig    Sample: adipose

Fig. 3. Western blotting analysis of ATGL, HSL and their phosphorylated protein contents in adipose tissue during processing of dry-cured meat. (A) WB image of each dry-cured group. (B) ATGL relative protein level in adipose tissue. (C) p-ATGL(S406) relative protein level in adipose tissue. (D) HSL relative protein level in adipose tissue. (E) p-HSL(Ser563) relative protein level in adipose tissue. (F) p-HSL(Ser660) relative protein level in adipose tissue.
2). The Effects of Erchen Decoction on Gut Microbiota and Lipid Metabolism Disorders in Zucker Diabetic Fatty Rats. Frontiers in Pharmacology, 2021 (PubMed: 34366839) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 3 ECD improved insulin signal transduction and decreased lipolysis in WAT of ZDF rats. (A) Representative image (bar: 100 µM) and (B) number and average area of adipocytes of WAT HE staining (n = 3. Z vs. L, ****p < 0.0001; EC vs. Z, ## p < 0.01, ### p < 0.001). (C) Representative bands and (D) relative protein expression of p-IRS1/IRS1, p-AKT/AKT, p-PKA/PKA, p-HSL/HSL, and ATGL. (E) Serum FFA concentration (n = 4. Z vs. L, **p < 0.01, ***p < 0.001, ****p < 0.0001; EC vs. Z, # p < 0.05, ## p < 0.01). (B), (D), and (E) were analyzed by one-way ANOVA.
3). Activation of Transcription Factor EB Is Associated With Adipose Tissue Lipolysis in Dairy Cows With Subclinical Ketosis. Frontiers in Veterinary Science, 2022 (PubMed: 35211541) [IF=3.2]

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